Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A5ED21 (NAPA_BRASB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:BBta_1862
OrganismBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]
Taxonomic identifier288000 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length834 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232Tat-type signal Potential
Chain33 – 834802Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000069709

Regions

Domain44 – 100574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding541Iron-sulfur (4Fe-4S) By similarity
Metal binding581Iron-sulfur (4Fe-4S) By similarity
Metal binding861Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A5ED21 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 2A73E0590EF976FB

FASTA83493,602
        10         20         30         40         50         60 
MTDMKIDRRQ MLKLEAAAIA AAAAGMPSTS LAANLVTERE ASELKWDKAA CRFCGTGCSV 

        70         80         90        100        110        120 
MVATKDNRVV ATHGDIKAEV NRGLNCVKGY FLSKIMYGHD RLTHPMLRKT NGKYDKNGEF 

       130        140        150        160        170        180 
TPVSWDEAFD IMALKFKDAL KKRGPSGVGM FGSGQWTIWE GYAASKLFKA GFRTNNIDPN 

       190        200        210        220        230        240 
ARHCMASAVA GMMRTFGIDE PAGCYDDIEA ADAFVLWGSN MAEMHPILWT RVTDRRLSAP 

       250        260        270        280        290        300 
HVKVAVLSTF EHRSFDLADI GMVFKPQTDL YLLNAIANHI IKTGRVNKDF VQAHTVFKKG 

       310        320        330        340        350        360 
QTDIGYGLRP EHPLQKKATG AAKANDATDI SFDEYAKFVS DYTLEKAAEM SGVPLNRLEA 

       370        380        390        400        410        420 
LAELYADPKT KVVSFWTMGF NQHTRGVWCN NLVYNIHLLT GKIAEAGNSP FSLTGQPSAC 

       430        440        450        460        470        480 
GTAREVGTFS HRLPADMVVT NKEHRTKAEH IWQLPEGTIP DKPGYHAVLQ SRMLKDGLLN 

       490        500        510        520        530        540 
AYWVQVNNNL QAGPNANEET YPGFRNPDNF IVVSDAYPSV TALAADLILP TAMWVEKEGA 

       550        560        570        580        590        600 
YGNAERRTQF WHQLVSAPGE ARSDLWQLME FSKRFKIEEV WPEELIAKKP DVRGKTLFDV 

       610        620        630        640        650        660 
LYKNGQVDKF PLDQIEAGYA NDESKAFGFY VHKGLFEEYA AFGRGHGHDL APFEAYHKER 

       670        680        690        700        710        720 
GLRWPVVDGK ETRWRFREGS DPYVKAGTGV QFYGFPDGKA RIFALPYEPP AESPDKDYPF 

       730        740        750        760        770        780 
WLSTGRVVEH WHSGTMTRRV PELYKAFPEA VCFMHPDDAQ EANLRRGDEV KVASRRGFIR 

       790        800        810        820        830 
ARVETRGRDK PPRGLVFVPW FDESKLINKV TLDATDPISL QTDYKKCAVR IERV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000494 Genomic DNA. Translation: ABQ34065.1.
RefSeqYP_001237971.1. NC_009485.1.

3D structure databases

ProteinModelPortalA5ED21.
SMRA5ED21. Positions 44-832.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING288000.BBta_1862.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ34065; ABQ34065; BBta_1862.
GeneID5154592.
KEGGbbt:BBta_1862.
PATRIC21204617. VBIBraSp29847_1994.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAHAMANVI.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycBSP288000:GJBR-1787-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_BRASB
AccessionPrimary (citable) accession number: A5ED21
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 12, 2007
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families