SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A5ED21

- NAPA_BRASB

UniProt

A5ED21 - NAPA_BRASB

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Periplasmic nitrate reductase
Gene
napA, BBta_1862
Organism
Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511Iron-sulfur (4Fe-4S) By similarity
Metal bindingi54 – 541Iron-sulfur (4Fe-4S) By similarity
Metal bindingi58 – 581Iron-sulfur (4Fe-4S) By similarity
Metal bindingi86 – 861Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciBSP288000:GJBR-1787-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:BBta_1862
OrganismiBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Taxonomic identifieri288000 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000000246: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signal Reviewed prediction
Add
BLAST
Chaini33 – 834802Periplasmic nitrate reductaseUniRule annotation
PRO_1000069709Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi288000.BBta_1862.

Structurei

3D structure databases

ProteinModelPortaliA5ED21.
SMRiA5ED21. Positions 44-832.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 100574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiHAMANVI.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5ED21-1 [UniParc]FASTAAdd to Basket

« Hide

MTDMKIDRRQ MLKLEAAAIA AAAAGMPSTS LAANLVTERE ASELKWDKAA    50
CRFCGTGCSV MVATKDNRVV ATHGDIKAEV NRGLNCVKGY FLSKIMYGHD 100
RLTHPMLRKT NGKYDKNGEF TPVSWDEAFD IMALKFKDAL KKRGPSGVGM 150
FGSGQWTIWE GYAASKLFKA GFRTNNIDPN ARHCMASAVA GMMRTFGIDE 200
PAGCYDDIEA ADAFVLWGSN MAEMHPILWT RVTDRRLSAP HVKVAVLSTF 250
EHRSFDLADI GMVFKPQTDL YLLNAIANHI IKTGRVNKDF VQAHTVFKKG 300
QTDIGYGLRP EHPLQKKATG AAKANDATDI SFDEYAKFVS DYTLEKAAEM 350
SGVPLNRLEA LAELYADPKT KVVSFWTMGF NQHTRGVWCN NLVYNIHLLT 400
GKIAEAGNSP FSLTGQPSAC GTAREVGTFS HRLPADMVVT NKEHRTKAEH 450
IWQLPEGTIP DKPGYHAVLQ SRMLKDGLLN AYWVQVNNNL QAGPNANEET 500
YPGFRNPDNF IVVSDAYPSV TALAADLILP TAMWVEKEGA YGNAERRTQF 550
WHQLVSAPGE ARSDLWQLME FSKRFKIEEV WPEELIAKKP DVRGKTLFDV 600
LYKNGQVDKF PLDQIEAGYA NDESKAFGFY VHKGLFEEYA AFGRGHGHDL 650
APFEAYHKER GLRWPVVDGK ETRWRFREGS DPYVKAGTGV QFYGFPDGKA 700
RIFALPYEPP AESPDKDYPF WLSTGRVVEH WHSGTMTRRV PELYKAFPEA 750
VCFMHPDDAQ EANLRRGDEV KVASRRGFIR ARVETRGRDK PPRGLVFVPW 800
FDESKLINKV TLDATDPISL QTDYKKCAVR IERV 834
Length:834
Mass (Da):93,602
Last modified:June 12, 2007 - v1
Checksum:i2A73E0590EF976FB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000494 Genomic DNA. Translation: ABQ34065.1.
RefSeqiWP_012042095.1. NC_009485.1.
YP_001237971.1. NC_009485.1.

Genome annotation databases

EnsemblBacteriaiABQ34065; ABQ34065; BBta_1862.
GeneIDi5154592.
KEGGibbt:BBta_1862.
PATRICi21204617. VBIBraSp29847_1994.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000494 Genomic DNA. Translation: ABQ34065.1 .
RefSeqi WP_012042095.1. NC_009485.1.
YP_001237971.1. NC_009485.1.

3D structure databases

ProteinModelPortali A5ED21.
SMRi A5ED21. Positions 44-832.
ModBasei Search...

Protein-protein interaction databases

STRINGi 288000.BBta_1862.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ34065 ; ABQ34065 ; BBta_1862 .
GeneIDi 5154592.
KEGGi bbt:BBta_1862.
PATRICi 21204617. VBIBraSp29847_1994.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi HAMANVI.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci BSP288000:GJBR-1787-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BTAi1 / ATCC BAA-1182.

Entry informationi

Entry nameiNAPA_BRASB
AccessioniPrimary (citable) accession number: A5ED21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 12, 2007
Last modified: September 3, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi