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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi54Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi58Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi86Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei88Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei155Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei180Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei184Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei378Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei382Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei488Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei537Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei564Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei800Substrate; via amide nitrogenUniRule annotation1
Binding sitei808Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei825Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:BBta_1862
OrganismiBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Taxonomic identifieri288000 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000000246 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Tat-type signalUniRule annotationAdd BLAST32
ChainiPRO_100006970933 – 834Periplasmic nitrate reductaseUniRule annotationAdd BLAST802

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi288000.BBta_1862

Structurei

3D structure databases

ProteinModelPortaliA5ED21
SMRiA5ED21
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 1004Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni217 – 224Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni248 – 252Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni267 – 269Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni514 – 515Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni724 – 733Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiANSMGTR
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5ED21-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDMKIDRRQ MLKLEAAAIA AAAAGMPSTS LAANLVTERE ASELKWDKAA
60 70 80 90 100
CRFCGTGCSV MVATKDNRVV ATHGDIKAEV NRGLNCVKGY FLSKIMYGHD
110 120 130 140 150
RLTHPMLRKT NGKYDKNGEF TPVSWDEAFD IMALKFKDAL KKRGPSGVGM
160 170 180 190 200
FGSGQWTIWE GYAASKLFKA GFRTNNIDPN ARHCMASAVA GMMRTFGIDE
210 220 230 240 250
PAGCYDDIEA ADAFVLWGSN MAEMHPILWT RVTDRRLSAP HVKVAVLSTF
260 270 280 290 300
EHRSFDLADI GMVFKPQTDL YLLNAIANHI IKTGRVNKDF VQAHTVFKKG
310 320 330 340 350
QTDIGYGLRP EHPLQKKATG AAKANDATDI SFDEYAKFVS DYTLEKAAEM
360 370 380 390 400
SGVPLNRLEA LAELYADPKT KVVSFWTMGF NQHTRGVWCN NLVYNIHLLT
410 420 430 440 450
GKIAEAGNSP FSLTGQPSAC GTAREVGTFS HRLPADMVVT NKEHRTKAEH
460 470 480 490 500
IWQLPEGTIP DKPGYHAVLQ SRMLKDGLLN AYWVQVNNNL QAGPNANEET
510 520 530 540 550
YPGFRNPDNF IVVSDAYPSV TALAADLILP TAMWVEKEGA YGNAERRTQF
560 570 580 590 600
WHQLVSAPGE ARSDLWQLME FSKRFKIEEV WPEELIAKKP DVRGKTLFDV
610 620 630 640 650
LYKNGQVDKF PLDQIEAGYA NDESKAFGFY VHKGLFEEYA AFGRGHGHDL
660 670 680 690 700
APFEAYHKER GLRWPVVDGK ETRWRFREGS DPYVKAGTGV QFYGFPDGKA
710 720 730 740 750
RIFALPYEPP AESPDKDYPF WLSTGRVVEH WHSGTMTRRV PELYKAFPEA
760 770 780 790 800
VCFMHPDDAQ EANLRRGDEV KVASRRGFIR ARVETRGRDK PPRGLVFVPW
810 820 830
FDESKLINKV TLDATDPISL QTDYKKCAVR IERV
Length:834
Mass (Da):93,602
Last modified:June 12, 2007 - v1
Checksum:i2A73E0590EF976FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000494 Genomic DNA Translation: ABQ34065.1
RefSeqiWP_012042095.1, NC_009485.1

Genome annotation databases

EnsemblBacteriaiABQ34065; ABQ34065; BBta_1862
KEGGibbt:BBta_1862

Similar proteinsi

Entry informationi

Entry nameiNAPA_BRASB
AccessioniPrimary (citable) accession number: A5ED21
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 12, 2007
Last modified: March 28, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health