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Protein

Ribonuclease H

Gene

rnhA

Organism
Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Magnesium 1UniRule annotation1
Metal bindingi12Magnesium 2UniRule annotation1
Metal bindingi50Magnesium 1UniRule annotation1
Metal bindingi72Magnesium 1UniRule annotation1
Metal bindingi136Magnesium 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease HUniRule annotation (EC:3.1.26.4UniRule annotation)
Short name:
RNase HUniRule annotation
Gene namesi
Name:rnhAUniRule annotation
Ordered Locus Names:BBta_0950
OrganismiBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Taxonomic identifieri288000 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000000246 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000746361 – 154Ribonuclease HAdd BLAST154

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi288000.BBta_0950.

Structurei

3D structure databases

ProteinModelPortaliA5EAL2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 144RNase HUniRule annotationAdd BLAST142

Sequence similaritiesi

Belongs to the RNase H family.UniRule annotation
Contains 1 RNase H domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UMW. Bacteria.
COG0328. LUCA.
HOGENOMiHOG000040465.
KOiK03469.
OMAiLVTDSQY.
OrthoDBiPOG091H04XT.

Family and domain databases

CDDicd09278. RNase_HI_prokaryote_like. 1 hit.
Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H. 1 hit.
InterProiIPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR022892. RNaseHI.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5EAL2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELPTVSIY TDGACSGNPG PGGWGAILRF GDKEKELKGG EPHTTNNRME
60 70 80 90 100
LMAAISALEA LKKSCQVELY TDSQYVRQGI TGWIHGWKRN GWKTADKKPV
110 120 130 140 150
KNAELWQRLD AALKPHKVNW HWVKGHAGHA ENERADQLAR DGVAMARLQK

NVRG
Length:154
Mass (Da):17,127
Last modified:June 12, 2007 - v1
Checksum:i6D19D7850F46DC0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000494 Genomic DNA. Translation: ABQ33206.1.
RefSeqiWP_012041254.1. NC_009485.1.

Genome annotation databases

EnsemblBacteriaiABQ33206; ABQ33206; BBta_0950.
KEGGibbt:BBta_0950.
PATRICi21202884. VBIBraSp29847_1137.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000494 Genomic DNA. Translation: ABQ33206.1.
RefSeqiWP_012041254.1. NC_009485.1.

3D structure databases

ProteinModelPortaliA5EAL2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi288000.BBta_0950.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ33206; ABQ33206; BBta_0950.
KEGGibbt:BBta_0950.
PATRICi21202884. VBIBraSp29847_1137.

Phylogenomic databases

eggNOGiENOG4108UMW. Bacteria.
COG0328. LUCA.
HOGENOMiHOG000040465.
KOiK03469.
OMAiLVTDSQY.
OrthoDBiPOG091H04XT.

Family and domain databases

CDDicd09278. RNase_HI_prokaryote_like. 1 hit.
Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H. 1 hit.
InterProiIPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR022892. RNaseHI.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNH_BRASB
AccessioniPrimary (citable) accession number: A5EAL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: November 2, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.