Malate dehydrogenase - Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	BBta_0392

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD⁺ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 322

322

Malate dehydrogenase HAMAP-Rule MF_00487

PRO_1000026466

Regions

Nucleotide binding

10 – 15

6

NAD By similarity

Nucleotide binding

119 – 121

3

NAD By similarity

Sites

Active site

176

1

Proton acceptor By similarity

Binding site

34

1

NAD By similarity

Binding site

83

1

Substrate By similarity

Binding site

89

1

Substrate By similarity

Binding site

96

1

NAD By similarity

Binding site

121

1

Substrate By similarity

Binding site

152

1

Substrate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

A5E935 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 23678283BBF1D08C
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FASTA

322

34,065

        10         20         30         40         50         60 
MARDKIALIG SGQIGGTLAH LIGLKELGDV VMFDIAEGVP QGKALDIAQS SPVDGFDAHY 

        70         80         90        100        110        120 
SGANSYEALD NAKVCIVTAG VPRKPGMSRD DLLSINLKVM EQVGAGIKKY APDAFVICIT 

       130        140        150        160        170        180 
NPLDAMVWAL QKASGLPAKK VVGMAGVLDS ARFRYFLADE FNVSVEDVTA FVLGGHGDTM 

       190        200        210        220        230        240 
VPLTKYSTVA GIPLPDLVKM GWTSQARIDE IVDRTRNGGA EIVNLLKTGS AYYAPAASAI 

       250        260        270        280        290        300 
AMAESYLRDK KRVLPCAAHL NGEFGVKDMY VGVPVVIGSK GVERIVEIEL AGKDREAFDK 

       310        320 
SVAAVQGLVD ACKKIAPDLL GR

« Hide

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia."
Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.

Sadowsky M.
Science 316:1307-1312(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BTAi1 / ATCC BAA-1182.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000494 Genomic DNA. Translation: ABQ32679.1.
RefSeq	YP_001236585.1. NC_009485.1.
3D structure databases
ProteinModelPortal	A5E935.
SMR	A5E935. Positions 1-313.
ModBase	Search...
Protein-protein interaction databases
STRING	288000.BBta_0392.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ32679; ABQ32679; BBta_0392.
GeneID	5154257.
KEGG	bbt:BBta_0392.
PATRIC	21201794. VBIBraSp29847_0597.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213794.
KO	K00024.
OMA	INDYEES.
ProtClustDB	PRK06223.
Enzyme and pathway databases
BioCyc	BSP288000:GJBR-379-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_BRASB

Accession

Primary (citable) accession number: A5E935

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents