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A5E915

- DAPF_BRASB

UniProt

A5E915 - DAPF_BRASB

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei17 – 171SubstrateUniRule annotation
    Binding sitei47 – 471SubstrateUniRule annotation
    Binding sitei67 – 671SubstrateUniRule annotation
    Active sitei76 – 761Proton donor/acceptorUniRule annotation
    Binding sitei164 – 1641SubstrateUniRule annotation
    Sitei166 – 1661Important for catalytic activityUniRule annotation
    Binding sitei198 – 1981SubstrateUniRule annotation
    Sitei216 – 2161Important for catalytic activityUniRule annotation
    Active sitei225 – 2251Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciBSP288000:GJBR-359-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:BBta_0372
    OrganismiBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
    Taxonomic identifieri288000 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000000246: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 289289Diaminopimelate epimerasePRO_1000011848Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi76 ↔ 225

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi288000.BBta_0372.

    Structurei

    3D structure databases

    ProteinModelPortaliA5E915.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni14 – 152Substrate bindingUniRule annotation
    Regioni76 – 783Substrate bindingUniRule annotation
    Regioni216 – 2172Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OMAiKMRIFNN.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5E915-1 [UniParc]FASTAAdd to Basket

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    MSALANHSFV KMNGIGNEIV VLDLRDVKHV VTPDEARAVA ARVPYDQLMV    50
    LQPPRLDGTE AFIRIYNNDG SESGACGNGM RCVVRQVFEK TGQASATFET 100
    RAGLLNCWQG PAPDLYTVDM GVPKFGWQEI PLAEEFRDTR YIELQIGPID 150
    APILHSPSVV NMGNPHAVFW VDGDVNSYDL ERFGPLLENH PIFPERANIT 200
    LAHIVDRDHI TMRTWERGAG LTKACGSAAC ATAVAAARLK RANRIVQMTL 250
    PGGELTIEWR ERDDHVLMTG TATFEFEGRF EPQLFASVA 289
    Length:289
    Mass (Da):31,947
    Last modified:June 12, 2007 - v1
    Checksum:i79B05221E93614BC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000494 Genomic DNA. Translation: ABQ32659.1.
    RefSeqiWP_012040712.1. NC_009485.1.
    YP_001236565.1. NC_009485.1.

    Genome annotation databases

    EnsemblBacteriaiABQ32659; ABQ32659; BBta_0372.
    GeneIDi5154441.
    KEGGibbt:BBta_0372.
    PATRICi21201754. VBIBraSp29847_0577.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000494 Genomic DNA. Translation: ABQ32659.1 .
    RefSeqi WP_012040712.1. NC_009485.1.
    YP_001236565.1. NC_009485.1.

    3D structure databases

    ProteinModelPortali A5E915.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 288000.BBta_0372.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ32659 ; ABQ32659 ; BBta_0372 .
    GeneIDi 5154441.
    KEGGi bbt:BBta_0372.
    PATRICi 21201754. VBIBraSp29847_0577.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OMAi KMRIFNN.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci BSP288000:GJBR-359-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BTAi1 / ATCC BAA-1182.

    Entry informationi

    Entry nameiDAPF_BRASB
    AccessioniPrimary (citable) accession number: A5E915
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3