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Protein

Sulfate adenylyltransferase subunit 2

Gene

cysD

Organism
Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sulfite from sulfate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 2 (cysD)
  2. no protein annotated in this organism
  3. Phosphoadenylylsulfate reductase (Thioredoxin) (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSP288000:GJBR-320-MONOMER.
UniPathwayiUPA00140; UER00204.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfate adenylyltransferase subunit 2UniRule annotation (EC:2.7.7.4UniRule annotation)
Alternative name(s):
ATP-sulfurylase small subunitUniRule annotation
Sulfate adenylate transferaseUniRule annotation
Short name:
SATUniRule annotation
Gene namesi
Name:cysDUniRule annotation
Ordered Locus Names:BBta_0328
OrganismiBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Taxonomic identifieri288000 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000000246 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Sulfate adenylyltransferase subunit 2PRO_0000340180Add
BLAST

Interactioni

Subunit structurei

Heterodimer composed of CysD, the smaller subunit, and CysN.UniRule annotation

Protein-protein interaction databases

STRINGi288000.BBta_0328.

Structurei

3D structure databases

ProteinModelPortaliA5E8X6.
SMRiA5E8X6. Positions 26-232.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PAPS reductase family. CysD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C11. Bacteria.
COG0175. LUCA.
HOGENOMiHOG000263604.
KOiK00957.
OMAiIWRYIEL.
OrthoDBiPOG091H03PV.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
HAMAPiMF_00064. Sulf_adenylyltr_sub2. 1 hit.
InterProiIPR002500. PAPS_reduct.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011784. SO4_adenylTrfase_ssu.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMsiTIGR02039. CysD. 1 hit.

Sequencei

Sequence statusi: Complete.

A5E8X6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLRHATEI ATTRWPAAAE PRPAALNHLH QLEAESIAIL REVAAEFRKP
60 70 80 90 100
VMLYSIGKDS SVLLHLAMKA FHPAKPPFPL LHIDTTWKFR DMIAFRDQRA
110 120 130 140 150
RELGLELLVH VNPEGLAQKI SPFTHGSALY TDVMKTQALR QALDAHGFDA
160 170 180 190 200
AIGGARRDEE KSRAKERIFS HRSATHRWDP KNQRPELWHL YNTMLAPGES
210 220 230 240 250
MRVFPLSNWT ELDVWDYIQL ERIPIVPLYF AARRPVVERD GALIMVDDER
260 270 280 290 300
MPLRPGETPQ WRSIRFRTLG CYPLTGATPS TASDLAAIIR EMRTSRTSER
310 320
QGRVIDRDSA ASMERKKVEG YF
Length:322
Mass (Da):36,795
Last modified:June 12, 2007 - v1
Checksum:iE40787C202A9A1F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000494 Genomic DNA. Translation: ABQ32620.1.
RefSeqiWP_012040674.1. NC_009485.1.

Genome annotation databases

EnsemblBacteriaiABQ32620; ABQ32620; BBta_0328.
KEGGibbt:BBta_0328.
PATRICi21201670. VBIBraSp29847_0535.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000494 Genomic DNA. Translation: ABQ32620.1.
RefSeqiWP_012040674.1. NC_009485.1.

3D structure databases

ProteinModelPortaliA5E8X6.
SMRiA5E8X6. Positions 26-232.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi288000.BBta_0328.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ32620; ABQ32620; BBta_0328.
KEGGibbt:BBta_0328.
PATRICi21201670. VBIBraSp29847_0535.

Phylogenomic databases

eggNOGiENOG4105C11. Bacteria.
COG0175. LUCA.
HOGENOMiHOG000263604.
KOiK00957.
OMAiIWRYIEL.
OrthoDBiPOG091H03PV.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204.
BioCyciBSP288000:GJBR-320-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
HAMAPiMF_00064. Sulf_adenylyltr_sub2. 1 hit.
InterProiIPR002500. PAPS_reduct.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011784. SO4_adenylTrfase_ssu.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMsiTIGR02039. CysD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCYSD_BRASB
AccessioniPrimary (citable) accession number: A5E8X6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 12, 2007
Last modified: September 7, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.