A5E8X6 (CYSD_BRASB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate adenylyltransferase subunit 2
EC=2.7.7.4

Alternative name(s):
ATP-sulfurylase small subunit
Sulfate adenylate transferase
Short name=SAT

Gene names

Name:	cysD
Ordered Locus Names:	BBta_0328

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of CysD, the smaller subunit, and CysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_0000340180

Sequences

	Sequence		Length	Mass (Da)
	A5E8X6 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: E40787C202A9A1F9	FASTA	322	36,795
	10 20 30 40 50 60 MSSLRHATEI ATTRWPAAAE PRPAALNHLH QLEAESIAIL REVAAEFRKP VMLYSIGKDS 70 80 90 100 110 120 SVLLHLAMKA FHPAKPPFPL LHIDTTWKFR DMIAFRDQRA RELGLELLVH VNPEGLAQKI 130 140 150 160 170 180 SPFTHGSALY TDVMKTQALR QALDAHGFDA AIGGARRDEE KSRAKERIFS HRSATHRWDP 190 200 210 220 230 240 KNQRPELWHL YNTMLAPGES MRVFPLSNWT ELDVWDYIQL ERIPIVPLYF AARRPVVERD 250 260 270 280 290 300 GALIMVDDER MPLRPGETPQ WRSIRFRTLG CYPLTGATPS TASDLAAIIR EMRTSRTSER 310 320 QGRVIDRDSA ASMERKKVEG YF

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BTAi1 / ATCC BAA-1182.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000494 Genomic DNA. Translation: ABQ32620.1.
RefSeq	YP_001236526.1. NC_009485.1.
3D structure databases
ProteinModelPortal	A5E8X6.
SMR	A5E8X6. Positions 26-232.
ModBase	Search...
Protein-protein interaction databases
STRING	A5E8X6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5153295.
GenomeReviews	Gene locus BBta_0328 in contig CP000494_GR.
KEGG	bbt:BBta_0328.
PATRIC	21201670. VBIBraSp29847_0535.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0175.
HOGENOM	HBG758022.
OMA	RVIDHDA.
ProtClustDB	PRK12563.
Family and domain databases
HAMAP	MF_00064. Sulf_adenylyltr_sub2. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K00957.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_BRASB

Accession

Primary (citable) accession number: A5E8X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 12, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program