A5E8V7 (PNCB_BRASB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotinate phosphoribosyltransferase
Short name=NAPRTase
EC=2.4.2.11

Gene names

Name:	pncB
Ordered Locus Names:	BBta_0308

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00570
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1. HAMAP-Rule MF_00570
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAPRTase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP nicotinate nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nicotinate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP nicotinate-nucleotide diphosphorylase (carboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Nicotinate phosphoribosyltransferase HAMAP-Rule MF_00570		PRO_1000146833

Sequences

	Sequence		Length	Mass (Da)
	A5E8V7 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 7A1EE8DE95BB03E1	FASTA	434	49,497
	10 20 30 40 50 60 MTVTDIASRT YNHGWRLDPI VRSLLDTDFY KLLMLQMIRE FYPDQKVTFS VINRTKRVRL 70 80 90 100 110 120 GDVIDEGELR AQLDHARTIR FTKKELIWLA GNTFYGKTHM FSPDFLAWLA HFRLPDYELH 130 140 150 160 170 180 KADGQYELHF HGPWTHTTMW EIPALAIVNE LRSRQAMKGQ GRFALDVLFA RAKAKLWAKV 190 200 210 220 230 240 ERLRRLEGLR LSDFGTRRRH GFLWQRWCVE AVKEGLGPSF TGTSNVLLAM DNDLEAIGTN 250 260 270 280 290 300 AHELPMVAAA LARDDDELRF APYRILDQWR QTYAGNLLIA LPDAFGTKAF LRDAPDWVAD 310 320 330 340 350 360 WTGFRPDSAP PIEAGEEIIA WWKSKGRDPK QKLLVFSDAM DVESIEQIHH RFSDRVRLSF 370 380 390 400 410 420 GWGTNLTNDF VGCAPDGSVD LDPISLVCKV TSVDGQPAVK LSDNPEKATG DPAEIARYLR 430 VFGDAGRVRT PVVV

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BTAi1 / ATCC BAA-1182.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000494 Genomic DNA. Translation: ABQ32601.1.
RefSeq	YP_001236507.1. NC_009485.1.
3D structure databases
ProteinModelPortal	A5E8V7.
SMR	A5E8V7. Positions 1-424.
ModBase	Search...
Protein-protein interaction databases
STRING	288000.BBta_0308.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ32601; ABQ32601; BBta_0308.
GeneID	5153185.
KEGG	bbt:BBta_0308.
PATRIC	21201632. VBIBraSp29847_0516.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1488.
HOGENOM	HOG000284929.
KO	K00763.
OMA	SFGWGTN.
ProtClustDB	PRK05321.
Enzyme and pathway databases
BioCyc	BSP288000:GJBR-301-MONOMER.
UniPathway	UPA00253; UER00457.
Family and domain databases
HAMAP	MF_00570. NAPRTase.
InterPro	IPR006406. Nic_PRibTrfase. IPR015977. Nic_PRibTrfase-like. IPR007229. Nic_PRibTrfase-rel. IPR002638. Quinolinate_PRibosylTrfase_C. [Graphical view]
PANTHER	PTHR11098:SF1. PTHR11098:SF1. 1 hit.
Pfam	PF04095. NAPRTase. 1 hit. [Graphical view]
PIRSF	PIRSF000484. NAPRT. 1 hit.
SUPFAM	SSF51690. Q_phspho_trans. 1 hit.
TIGRFAMs	TIGR01514. NAPRTase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PNCB_BRASB

Accession

Primary (citable) accession number: A5E8V7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	June 12, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program