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A5E8H4 (SYL_BRASB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BBta_0171
OrganismBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]
Taxonomic identifier288000 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length874 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 874874Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009300

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif630 – 6345"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6331ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5E8H4 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 5AA7D708047FC63D

FASTA87497,492
        10         20         30         40         50         60 
MTSERYNARE AEPRWQRQWD EKAIFATKND DPRPKYYVLE MFPYPSGRIH IGHVRNYTLG 

        70         80         90        100        110        120 
DVLARFMRAK GFNVLHPMGW DAFGLPAENA AIERKVAPKA WTYDNIAAMK KQLRSIGLSL 

       130        140        150        160        170        180 
DWAREFATCD PSYYKHQQKM FLDFMQAGLV EREQRKVNWD PVDMTVLANE QVIDGRGWRS 

       190        200        210        220        230        240 
GAVVEQREMN QWVFKITKYS QELLDALDGL DRWPDKVRLM QRNWIGRSEG LLIRFALDPQ 

       250        260        270        280        290        300 
TTPAGETELK IFTTRPDTLF GAKFMAISAD HPLAQAAAAK NPELAAFIAE IKRIGTAQEA 

       310        320        330        340        350        360 
IDTAEKQGFD TGIRAVHPFD PSWTLPVYVA NFVLMEYGTG AIFGCPAHDQ RDLDFVNKYG 

       370        380        390        400        410        420 
LGNTPVVCPE GQDPASFVIT DTAFDGDGRL INSRFLDGMT IAQAKDEVAK RLEAEQRGGA 

       430        440        450        460        470        480 
AVGERQVNFR LRDWGISRQR YWGCPIPVIH CPTCDVVPVP AKDLPVVLPD DVTFDKPGNA 

       490        500        510        520        530        540 
LDHHPTWKHV TCPKCGGKAT RETDTMDTFV DSSWYFARFT DPWNETAPTT PDVANRMMPV 

       550        560        570        580        590        600 
DQYIGGVEHA ILHLLYSRFF TRAMKATGHV AMDEPFAGMF TQGMVVHETY QKADGTYVNP 

       610        620        630        640        650        660 
AEVKIEVGGN GRRAVLTATG EDITIGPIEK MSKSKKNTVD PDDIIESYGA DVARWFMLSD 

       670        680        690        700        710        720 
SPPDRDVIWS DERVQGAARF VQRLWRLVNE SVAAGQEAPS SRPATFGPDA LALRKAAHGA 

       730        740        750        760        770        780 
LDKVTGGIER LHFNVCLAHI REFANALAEV LARPAKPAPD LAWAIREAAQ ILVQLFSPMM 

       790        800        810        820        830        840 
PHLAEECWQV LGQGGLISEA SWPQIERDLL VEDSVTLVVQ VNGKKRGEVT VASNAQNPEI 

       850        860        870 
ESAVLALDAV KLALDGKPVR KVIIVPKRIV NVVG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000494 Genomic DNA. Translation: ABQ32468.1.
RefSeqYP_001236374.1. NC_009485.1.

3D structure databases

ProteinModelPortalA5E8H4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING288000.BBta_0171.

Proteomic databases

PRIDEA5E8H4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ32468; ABQ32468; BBta_0171.
GeneID5150375.
KEGGbbt:BBta_0171.
PATRIC21201342. VBIBraSp29847_0375.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycBSP288000:GJBR-164-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BRASB
AccessionPrimary (citable) accession number: A5E8H4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries