A5E8G9 (RSMG_BRASB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase G
EC=2.1.1.170

Alternative name(s):
16S rRNA 7-methylguanosine methyltransferase
Short name=16S rRNA m7G methyltransferase

Gene names

Name:	rsmG
Ordered Locus Names:	BBta_0166

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA By similarity. HAMAP MF_00074
Catalytic activity	S-adenosyl-L-methionine + guanosine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanosine(527) in 16S rRNA. HAMAP MF_00074
Subcellular location	Cytoplasm Potential HAMAP MF_00074.
Sequence similarities	Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Ribosomal RNA small subunit methyltransferase G HAMAP MF_00074		PRO_0000335313

Sequences

	Sequence		Length	Mass (Da)
	A5E8G9 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 2C15A80A926F006A	FASTA	222	23,999
	10 20 30 40 50 60 MKQTSAVVAS KVIAADKAAA LALTPVSRET EQRLDRYVAL LLEWQAKTNL VAPSTLPHLW 70 80 90 100 110 120 TRHISDSLQL LDLAPDAKIW VDLGSGGGFP GVVLACALAE RPGAEVHLVE RIAKKAAFLR 130 140 150 160 170 180 EAIRVTGAPG IVHLSEIGDI VEKWSGGVDC VTARALAPLH QLIGFAEPLV KRGAKALFLK 190 200 210 220 GQDVEAELTE STKYWKIEPK LYSSRTGGQG WIVAIDCIER HN

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BTAi1 / ATCC BAA-1182.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000494 Genomic DNA. Translation: ABQ32463.1.
RefSeq	YP_001236369.1. NC_009485.1.
3D structure databases
ProteinModelPortal	A5E8G9.
ModBase	Search...
Protein-protein interaction databases
STRING	A5E8G9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5149182.
GenomeReviews	Gene locus BBta_0166 in contig CP000494_GR.
KEGG	bbt:BBta_0166.
PATRIC	21201330. VBIBraSp29847_0369.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0357.
HOGENOM	HBG686577.
OMA	GGQGWIV.
ProtClustDB	PRK00107.
Family and domain databases
HAMAP	MF_00074. 16SrRNA_methyltr_G. [Tree]
InterPro	IPR003682. rRNA_ssu_MeTfrase_G. [Graphical view]
KO	K03501.
Pfam	PF02527. GidB. 1 hit. [Graphical view]
PIRSF	PIRSF003078. GidB. 1 hit.
TIGRFAMs	TIGR00138. GidB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMG_BRASB

Accession

Primary (citable) accession number: A5E8G9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 12, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program