Reviewed, UniProtKB/Swiss-Prot A5E8B1 (AROA_BRASB)

Names and origin

Protein names

Recommended name:
    3-phosphoshikimate 1-carboxyvinyltransferase
    EC=2.5.1.19
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate synthase
      Short name=EPSP synthase
      Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	BBta_0105

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm Probable HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000325336

Sequences

	Sequence		Length	Mass (Da)
	A5E8B1-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: EAE6AD7B97A85711	FASTA	445	46,303
	10 20 30 40 50 60 MSAPAAPIPL ESRASGPLSG TLRVPGDKSI SHRALILGAL SVGETRISGL LEGEDVLNTA 70 80 90 100 110 120 QAMRALGAKV ERHGDFAWTV HGVGVGGFAQ PAATLDFGNS GTGCRLVMGA VAGCPITAVF 130 140 150 160 170 180 DGDASLRSRP MRRILDPLEL IGAKVTASAE GGKLPLTLQG ASNPVPVVYR TPVASAQIKS 190 200 210 220 230 240 AVLLAGLAAP GVTTVIEQEA SRDHTELMLK HFGAEIVTTL EGTHGRRIAL TGQPELHGAP 250 260 270 280 290 300 VIVPADPSSA AFPLVAALIV DGSDLVLSDV MTNPLRTGLF ATLREMGASI EEDDVRGDAG 310 320 330 340 350 360 EPMVRLRVRA SKLRGVEVPP ERAPSMIDEY LVLAVAAAYA EGTTIMRGLH ELRVKESDRL 370 380 390 400 410 420 EATAAMLRVN GVKVEISDDD LIVEGRGHVP GGGLVATHMD HRIAMSALVM GLASDKPVKV 430 440 DDTAFIATSF PDFVPLMCKA GADFA

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000494 Genomic DNA. Translation: ABQ32405.1.
RefSeq	YP_001236311.1.
3D structure databases
SMR	A5E8B1. Positions 9-444.
ModBase	Search...
Protein-protein interaction databases
STRING	A5E8B1.
Genome annotation databases
GeneID	5155296.
GenomeReviews	Gene locus BBta_0105 in contig CP000494_GR.
KEGG	bbt:BBta_0105.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0128.
HOGENOM	HBG646626.
OMA	DVEIRAD.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR016228. EPSP_synthase. IPR001986. EPSP_synthase_core. IPR006264. EPSP_synthase_subgroup. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
TIGRFAMs	TIGR01356. aroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_BRASB

Accession

Primary (citable) accession number: A5E8B1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	June 12, 2007
Last modified:	February 9, 2010
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)