ID   NCB5R_LODEL             Reviewed;         300 AA.
AC   A5E7U2;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   16-JUN-2009, entry version 17.
DE   RecName: Full=NADH-cytochrome b5 reductase 1;
DE            EC=1.6.2.2;
DE   AltName: Full=Microsomal cytochrome b reductase;
GN   Name=CBR1; ORFNames=LELG_05681;
OS   Lodderomyces elongisporus (Yeast) (Saccharomyces elongisporus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Lodderomyces.
OX   NCBI_TaxID=379508;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 11503 / CBS 2605 / IFO 1676 / JCM 1781 / NRRL YB-4239;
RA   Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K.,
RA   Cuomo C., Kellis M., Rasmussen M.D., Grochow J.A., Jaffe D.B.,
RA   Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M.,
RA   Mauceli E.W., Brockman W., MacCallum I.A., Rounsley S., Young S.K.,
RA   LaButti K., Pushparaj V., DeCaprio D., Crawford M., Koehrsen M.,
RA   Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S.,
RA   White J., Zeng Q., Kodira C.D., Yandava C., Alvarado L., O'Leary S.,
RA   Kurtzman C., Reedy J., Heitman J.;
RT   "The genome sequence of Lodderomyces elongisporus.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Electron donor reductase for cytochrome b5. The
CC       cytochrome b5/NADH cytochrome b5 reductase electron transfer
CC       system supports the catalytic activity of several sterol
CC       biosynthetic enzymes (By similarity).
CC   -!- CATALYTIC ACTIVITY: NADH + 2 ferricytochrome b5 = NAD(+) + H(+) +
CC       2 ferrocytochrome b5.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC       membrane protein (By similarity). Mitochondrion outer membrane;
CC       Single-pass membrane protein (By similarity).
CC   -!- SIMILARITY: Belongs to the flavoprotein pyridine nucleotide
CC       cytochrome reductase family.
CC   -!- SIMILARITY: Contains 1 FAD-binding FR-type domain.
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DR   EMBL; CH981534; EDK47500.1; -; Genomic_DNA.
DR   RefSeq; XP_001523135.1; -.
DR   GeneID; 5230252; -.
DR   OMA; A5E7U2; GNISRHV.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004128; F:cytochrome-b5 reductase activity; IEA:EC.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR017927; Fd_Rdtase_FAD-bd.
DR   InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR   InterPro; IPR001834; NADH-Cyt_B5_reductase.
DR   InterPro; IPR008333; OxRdtase_FAD-bd.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD_bd.
DR   Pfam; PF00970; FAD_binding_6; 1.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   PRINTS; PR00406; CYTB5RDTASE.
DR   PRINTS; PR00371; FPNCR.
DR   PROSITE; PS51384; FAD_FR; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Endoplasmic reticulum; FAD; Flavoprotein; Membrane;
KW   Mitochondrion; Mitochondrion outer membrane; NAD; Oxidoreductase;
KW   Transmembrane.
FT   CHAIN         1    300       NADH-cytochrome b5 reductase 1.
FT                                /FTId=PRO_0000330156.
FT   TRANSMEM      8     28       Potential.
FT   DOMAIN       57    160       FAD-binding FR-type.
FT   NP_BIND     140    155       FAD (By similarity).
FT   NP_BIND     166    198       FAD (By similarity).
FT   COMPBIAS     38     47       Poly-Ser.
SQ   SEQUENCE   300 AA;  32936 MW;  CFB31BB114073312 CRC64;
     MAETETNPLV VFATVATIII SFVTLYFFKQ SAKSSTTSSS SSSSSKSKKG SPALIPDKFQ
     KFPLISKTQV SHNSAIYRFG LPNPTDTLNL PIGQHISIGT IIDGKEVVRS YTPISLGDQQ
     GHFDLLIKTY ENGNISRHVA EKQVGDFVEI RGPKGFFTYT PNMKKSLGLI AGGTGIAPMY
     QIITAIMNNP EDKTKVHLLY ANVTENDILL RDELEQYAKE HPDRLKIHHV LNEAPEGWQH
     LTGFVTPELI DKHLPKPSAD TNLLLCGPPP MISAMKKAAV SLGFDKAKPV SKLGDQVFVF
//