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A5E7T4

- TRMB_LODEL

UniProt

A5E7T4 - TRMB_LODEL

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) (Yeast) (Saccharomyces elongisporus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei121 – 1211S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei200 – 2001S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei203 – 2031UniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    Transfer RNA methyltransferase 8UniRule annotation
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:TRM8UniRule annotation
    ORF Names:LELG_05673
    OrganismiLodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) (Yeast) (Saccharomyces elongisporus)
    Taxonomic identifieri379508 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeLodderomyces
    ProteomesiUP000001996: Unassembled WGS sequence

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 306306tRNA (guanine-N(7)-)-methyltransferasePRO_0000370598Add
    BLAST

    Interactioni

    Subunit structurei

    Forms a complex with TRM82.UniRule annotation

    Protein-protein interaction databases

    STRINGi36914.A5E7T4.

    Structurei

    3D structure databases

    ProteinModelPortaliA5E7T4.
    SMRiA5E7T4. Positions 82-302.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni144 – 1452S-adenosyl-L-methionine bindingUniRule annotation
    Regioni180 – 1812S-adenosyl-L-methionine bindingUniRule annotation
    Regioni278 – 2803S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    KOiK03439.
    OMAiRAHSNPI.
    OrthoDBiEOG708W9T.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5E7T4-1 [UniParc]FASTAAdd to Basket

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    MSSTAPLDSK ATEQITTAAE SKHNETQATR RKRYREAQEQ TRNKHLRFQA    50
    SPTPEEASPE LPSDEVAKLP QKRFYRQRAH SNPFSDHNLE YPVLPSQMDW 100
    STIYPHYTPA SLRKVEIADI GCGFGGLLID LGPQFPESLI LGMEIRVQVT 150
    NYVQDRLIAL REIHKADAYN YDNLGVIRGN AMKFLPNFFT KGQLSKMFFC 200
    FPDPHFKQRK HKARIITNTL LSEYAYVLRE GGVVYTITDV EDLHNWMVKH 250
    LDEHPLFERL SKEWEEQDPC VKIMYNSTEE GQKVARNQGS KYVACYKRLP 300
    NPDDCE 306
    Length:306
    Mass (Da):35,413
    Last modified:June 12, 2007 - v1
    Checksum:i2F6E9D4FCCD03E63
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH981534 Genomic DNA. Translation: EDK47492.1.
    RefSeqiXP_001523127.1. XM_001523077.1.

    Genome annotation databases

    GeneIDi5230260.
    KEGGilel:LELG_05673.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH981534 Genomic DNA. Translation: EDK47492.1 .
    RefSeqi XP_001523127.1. XM_001523077.1.

    3D structure databases

    ProteinModelPortali A5E7T4.
    SMRi A5E7T4. Positions 82-302.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 36914.A5E7T4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5230260.
    KEGGi lel:LELG_05673.

    Phylogenomic databases

    eggNOGi COG0220.
    KOi K03439.
    OMAi RAHSNPI.
    OrthoDBi EOG708W9T.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239.

    Entry informationi

    Entry nameiTRMB_LODEL
    AccessioniPrimary (citable) accession number: A5E7T4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3