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A5E7T4

- TRMB_LODEL

UniProt

A5E7T4 - TRMB_LODEL

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) (Yeast) (Saccharomyces elongisporus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei121 – 1211S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei200 – 2001S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei203 – 2031UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:TRM8UniRule annotation
ORF Names:LELG_05673
OrganismiLodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) (Yeast) (Saccharomyces elongisporus)
Taxonomic identifieri379508 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeLodderomyces
ProteomesiUP000001996: Unassembled WGS sequence

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 306306tRNA (guanine-N(7)-)-methyltransferasePRO_0000370598Add
BLAST

Interactioni

Subunit structurei

Forms a complex with TRM82.UniRule annotation

Protein-protein interaction databases

STRINGi36914.A5E7T4.

Structurei

3D structure databases

ProteinModelPortaliA5E7T4.
SMRiA5E7T4. Positions 82-302.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni144 – 1452S-adenosyl-L-methionine bindingUniRule annotation
Regioni180 – 1812S-adenosyl-L-methionine bindingUniRule annotation
Regioni278 – 2803S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
InParanoidiA5E7T4.
KOiK03439.
OMAiRAHSNPI.
OrthoDBiEOG708W9T.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5E7T4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSTAPLDSK ATEQITTAAE SKHNETQATR RKRYREAQEQ TRNKHLRFQA
60 70 80 90 100
SPTPEEASPE LPSDEVAKLP QKRFYRQRAH SNPFSDHNLE YPVLPSQMDW
110 120 130 140 150
STIYPHYTPA SLRKVEIADI GCGFGGLLID LGPQFPESLI LGMEIRVQVT
160 170 180 190 200
NYVQDRLIAL REIHKADAYN YDNLGVIRGN AMKFLPNFFT KGQLSKMFFC
210 220 230 240 250
FPDPHFKQRK HKARIITNTL LSEYAYVLRE GGVVYTITDV EDLHNWMVKH
260 270 280 290 300
LDEHPLFERL SKEWEEQDPC VKIMYNSTEE GQKVARNQGS KYVACYKRLP

NPDDCE
Length:306
Mass (Da):35,413
Last modified:June 12, 2007 - v1
Checksum:i2F6E9D4FCCD03E63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH981534 Genomic DNA. Translation: EDK47492.1.
RefSeqiXP_001523127.1. XM_001523077.1.

Genome annotation databases

GeneIDi5230260.
KEGGilel:LELG_05673.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH981534 Genomic DNA. Translation: EDK47492.1 .
RefSeqi XP_001523127.1. XM_001523077.1.

3D structure databases

ProteinModelPortali A5E7T4.
SMRi A5E7T4. Positions 82-302.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 36914.A5E7T4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5230260.
KEGGi lel:LELG_05673.

Phylogenomic databases

eggNOGi COG0220.
InParanoidi A5E7T4.
KOi K03439.
OMAi RAHSNPI.
OrthoDBi EOG708W9T.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239.

Entry informationi

Entry nameiTRMB_LODEL
AccessioniPrimary (citable) accession number: A5E7T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: June 12, 2007
Last modified: October 29, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3