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A5E7T4

- TRMB_LODEL

UniProt

A5E7T4 - TRMB_LODEL

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
TRM8, LELG_05673
Organism
Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) (Yeast) (Saccharomyces elongisporus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei121 – 1211S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei200 – 2001S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei203 – 2031 By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    Transfer RNA methyltransferase 8
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:TRM8
    ORF Names:LELG_05673
    OrganismiLodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) (Yeast) (Saccharomyces elongisporus)
    Taxonomic identifieri379508 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeLodderomyces
    ProteomesiUP000001996: Unassembled WGS sequence

    Subcellular locationi

    Nucleus By similarity UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 306306tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    PRO_0000370598Add
    BLAST

    Interactioni

    Subunit structurei

    Forms a complex with TRM82 By similarity.UniRule annotation

    Protein-protein interaction databases

    STRINGi36914.A5E7T4.

    Structurei

    3D structure databases

    ProteinModelPortaliA5E7T4.
    SMRiA5E7T4. Positions 82-302.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni144 – 1452S-adenosyl-L-methionine binding By similarity
    Regioni180 – 1812S-adenosyl-L-methionine binding By similarity
    Regioni278 – 2803S-adenosyl-L-methionine binding By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    KOiK03439.
    OMAiRAHSNPI.
    OrthoDBiEOG708W9T.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5E7T4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSTAPLDSK ATEQITTAAE SKHNETQATR RKRYREAQEQ TRNKHLRFQA    50
    SPTPEEASPE LPSDEVAKLP QKRFYRQRAH SNPFSDHNLE YPVLPSQMDW 100
    STIYPHYTPA SLRKVEIADI GCGFGGLLID LGPQFPESLI LGMEIRVQVT 150
    NYVQDRLIAL REIHKADAYN YDNLGVIRGN AMKFLPNFFT KGQLSKMFFC 200
    FPDPHFKQRK HKARIITNTL LSEYAYVLRE GGVVYTITDV EDLHNWMVKH 250
    LDEHPLFERL SKEWEEQDPC VKIMYNSTEE GQKVARNQGS KYVACYKRLP 300
    NPDDCE 306
    Length:306
    Mass (Da):35,413
    Last modified:June 12, 2007 - v1
    Checksum:i2F6E9D4FCCD03E63
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH981534 Genomic DNA. Translation: EDK47492.1.
    RefSeqiXP_001523127.1. XM_001523077.1.

    Genome annotation databases

    GeneIDi5230260.
    KEGGilel:LELG_05673.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH981534 Genomic DNA. Translation: EDK47492.1 .
    RefSeqi XP_001523127.1. XM_001523077.1.

    3D structure databases

    ProteinModelPortali A5E7T4.
    SMRi A5E7T4. Positions 82-302.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 36914.A5E7T4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5230260.
    KEGGi lel:LELG_05673.

    Phylogenomic databases

    eggNOGi COG0220.
    KOi K03439.
    OMAi RAHSNPI.
    OrthoDBi EOG708W9T.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239.

    Entry informationi

    Entry nameiTRMB_LODEL
    AccessioniPrimary (citable) accession number: A5E7T4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: June 12, 2007
    Last modified: June 11, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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