A5E567 (ATG15_LODEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative lipase ATG15
EC=3.1.1.3

Alternative name(s):
Autophagy-related protein 15

Gene names

Name:	ATG15
ORF Names:	LELG_04756

Organism

Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) (Yeast) (Saccharomyces elongisporus) [Complete proteome]

Taxonomic identifier

379508 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Lodderomyces

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW multivesicular body membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Putative lipase ATG15		PRO_0000317964
Regions
	Topological domain	1 – 26	26	Cytoplasmic By similarity
	Transmembrane	27 – 49	23	Helical; Signal-anchor for type II membrane protein
	Topological domain	50 – 552	503	Lumenal By similarity
Sites
	Active site	409	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	63	1	N-linked (GlcNAc...) Potential
	Glycosylation	147	1	N-linked (GlcNAc...) Potential
	Glycosylation	239	1	N-linked (GlcNAc...) Potential
	Glycosylation	307	1	N-linked (GlcNAc...) Potential
	Glycosylation	391	1	N-linked (GlcNAc...) Potential
	Glycosylation	526	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A5E567 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 141395528854882B	FASTA	552	63,136
	10 20 30 40 50 60 MLHITEKEQT RGLRQLEKRG KRLLPPLIKF IWFCLISAAC VAATTFYWLR LSPVHNIHKH 70 80 90 100 110 120 SLNSSFEDKG EDGTLQLKHI FHHGVGEKDY KIHKRLDVTQ EYLIKHSAYF QNMVNEQQQE 130 140 150 160 170 180 VQEIQEIHKR HEKQEIQVKY GPDQEYNQTN TALNSVGTEY KSRLSTNEYD WPDVHRGKNP 190 200 210 220 230 240 FDIQLPFKNK QSIARRIKHR NEPFFIESYL DYARSVNGDA LILNRINLEW IDDDINIPNI 250 260 270 280 290 300 TDKDTIVTLA TISSNAYVRF PQNDDDKKKS DWTDVGGGWI PDEENNDVNF GWEDVGLRGH 310 320 330 340 350 360 IFVSKDNKTV VIGIKGTSGA GLPGGGSDET TKNDKTNDNL LFSCCCARVG YMWTTVCDCY 370 380 390 400 410 420 EKAYTCNQDC LEKELRRKDR YYEAALDIYK NVTAIYPPET TDIWVTGHSL GGALASLLGR 430 440 450 460 470 480 TYGLPTVAYE APGEMLALKR LHLPQAPGLP RHLEHIWHIG NTADPIYMGV CNGASSSCSL 490 500 510 520 530 540 GGYAMETACH TGYQCVYDVV TDYGWRVNLL NHRIHTVIDD IILVYNETAP CVYQAPCRDC 550 FNWRFVSHDD KE

References

[1]

"Evolution of pathogenicity and sexual reproduction in eight Candida genomes." Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S., Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I., Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C., Fitzpatrick D.A., de Groot P.W.J. , Harris D., Hoyer L.L., Hube B., Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M., Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F., Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D., Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T., Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C., Birren B.W., Kellis M., Cuomo C.A. Nature 459:657-662(2009) [PubMed: 19465905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH981530 Genomic DNA. Translation: EDK46575.1.
RefSeq	XP_001523943.1. XM_001523893.1.
3D structure databases
ProteinModelPortal	A5E567.
ModBase	Search...
Protein-protein interaction databases
STRING	A5E567.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5231224.
KEGG	lel:LELG_04756.
Phylogenomic databases
OMA	METACHT.
OrthoDB	EOG4F7RV2.
Family and domain databases
InterPro	IPR002921. Lipase_3. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_LODEL

Accession

Primary (citable) accession number: A5E567

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 12, 2007
Last modified:	July 27, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program