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A5E450

- LIPA_LODEL

UniProt

A5E450 - LIPA_LODEL

Protein

Lipoyl synthase, mitochondrial

Gene

LELG_04389

Organism
Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) (Yeast) (Saccharomyces elongisporus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi150 – 1501Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi155 – 1551Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi161 – 1611Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi180 – 1801Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi184 – 1841Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi187 – 1871Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    ORF Names:LELG_04389
    OrganismiLodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) (Yeast) (Saccharomyces elongisporus)
    Taxonomic identifieri379508 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeLodderomyces
    ProteomesiUP000001996: Unassembled WGS sequence

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 432Lipoyl synthase, mitochondrialPRO_0000398271
    Transit peptidei1 – ?MitochondrionUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi36914.A5E450.

    Structurei

    3D structure databases

    ProteinModelPortaliA5E450.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    KOiK03644.
    OMAiRKVDCDI.
    OrthoDBiEOG79KPR7.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A5E450-1 [UniParc]FASTAAdd to Basket

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    MLPRSINILK LRPTTQVTTT ALRALATEAK TTLGATPGST STSTSTSTAT    50
    TTTLESTSTS TSGDATETTI KETKSIKRKR TFFTDELNKG PSFDDFVSGK 100
    AKDMLEDPLE LARKDPNAKL PLWLKVPIPK GKSFHNVKND VRELKLSTVC 150
    EEAKCPNIGE CWGGKKSEAT ATIMLLGDTC TRGCRFCSVK TNRKPGKPDP 200
    MEPENTAEAI SRWGLGYVVL TTVDRDDLID GGANHLKETV QKIKFKAPQI 250
    LVEVLGGDFR GDLEMVKILA NSGLDVYAHN METVEALTPH IRDRRATYRQ 300
    SLAVLRTAKE TKPSLITKTS LMLGFGETDD QIRQTLKDLR EVGCDVVTFG 350
    QYMRPTKRHM KVVEYVTPEK FDYWRDVALE MGFLYVASGP LVRSSYKAGE 400
    AFIENVLKKR KHNVGESPRM LQEVKPSIFK RA 432
    Length:432
    Mass (Da):47,959
    Last modified:June 12, 2007 - v1
    Checksum:i6371CA6B2C363D7C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH981529 Genomic DNA. Translation: EDK46208.1.
    RefSeqiXP_001524417.1. XM_001524367.1.

    Genome annotation databases

    GeneIDi5231419.
    KEGGilel:LELG_04389.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH981529 Genomic DNA. Translation: EDK46208.1 .
    RefSeqi XP_001524417.1. XM_001524367.1.

    3D structure databases

    ProteinModelPortali A5E450.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 36914.A5E450.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5231419.
    KEGGi lel:LELG_04389.

    Phylogenomic databases

    eggNOGi COG0320.
    KOi K03644.
    OMAi RKVDCDI.
    OrthoDBi EOG79KPR7.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239.

    Entry informationi

    Entry nameiLIPA_LODEL
    AccessioniPrimary (citable) accession number: A5E450
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3