A5E032 (MCES_LODEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
mRNA cap guanine-N7 methyltransferase
EC=2.1.1.56

Alternative name(s):
mRNA (guanine-N(7)-)-methyltransferase
mRNA cap methyltransferase

Gene names

Name:	ABD1
ORF Names:	LELG_02969

Organism

Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) (Yeast) (Saccharomyces elongisporus) [Complete proteome]

Taxonomic identifier

379508 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Lodderomyces

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for methylating the 5'-cap structure of mRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. mRNA cap methyltransferase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	mRNA cap guanine-N7 methyltransferase		PRO_0000303910
Regions
	Compositional bias	132 – 136	5	Poly-Ser

Sequences

	Sequence		Length	Mass (Da)
	A5E032 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: A43589215B5E67CC	FASTA	572	64,498
	10 20 30 40 50 60 MPEDSYIPQA KNDGAFQDLK RRRANDGHTE FSRRDRVHDV QPASIYSNVK ENKPAWMRSA 70 80 90 100 110 120 DENKDKKYDQ YGSRVDNGTL QQSLGVAKQV PTASASTTTV SAKVESFSAE KAINTANPPP 130 140 150 160 170 180 PSTTTTPSST TSSSSSFPSS SSLYLKYGNI GLGGRGTDPR MDRVKRNREQ LEQSLSIREH 190 200 210 220 230 240 LAVEENVNDE GSQPKGDGEV NPYLHLDVAN PSVIHTQRDE AHYRTFHSKI SDRENRDINS 250 260 270 280 290 300 IVRQHYNERT QQSKRQGRRT MSPIYKLRNF NNTIKYILLG NWAKYSSAEG NAPKIFSVLD 310 320 330 340 350 360 LCCGKGGDLN KCEFIEIDQY IGIDISDLSV REAFSRYSKQ KARFKSHSGA RTANKYNFEA 370 380 390 400 410 420 CFATGDCFTE TVPDILEPNF PGIIDQAFPV DAVSIQFALH YAFETEEKVR ALLVNVAKSL 430 440 450 460 470 480 RVGGTFIGTI PSSDFIRSKI VEKNILKDEN GKFKFGNSLY SATFDKEPPA DGVFRPAFGN 490 500 510 520 530 540 RYTYWLKDAV DNVPEYVVPF ETLRALCEEY NMTLRYKKNF IDVFNQEIPK YFSKLNKSLV 550 560 570 EGLKRSDGKY GAEGEEKEAV AFYVAFVFEK VT

References

[1]

"Evolution of pathogenicity and sexual reproduction in eight Candida genomes." Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S., Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I., Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C., Fitzpatrick D.A., de Groot P.W.J. , Harris D., Hoyer L.L., Hube B., Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M., Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F., Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D., Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T., Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C., Birren B.W., Kellis M., Cuomo C.A. Nature 459:657-662(2009) [PubMed: 19465905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH981526 Genomic DNA. Translation: EDK44790.1.
RefSeq	XP_001526411.1. XM_001526361.1.
3D structure databases
ProteinModelPortal	A5E032.
ModBase	Search...
Protein-protein interaction databases
STRING	A5E032.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5232939.
KEGG	lel:LELG_02969.
Phylogenomic databases
OMA	NFPGIID.
OrthoDB	EOG4CZFR2.
Family and domain databases
InterPro	IPR016899. mRNA_G-N7_MeTrfase. IPR004971. Pox_MCEL. [Graphical view]
KO	K00565.
Pfam	PF03291. Pox_MCEL. 1 hit. [Graphical view]
PIRSF	PIRSF028762. ABD1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCES_LODEL

Accession

Primary (citable) accession number: A5E032

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	June 12, 2007
Last modified:	November 16, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program