Reviewed, UniProtKB/Swiss-Prot A5DQE4 (MCR1_PICGU)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 2
    EC=1.6.2.2
Alternative name(s):
    Mitochondrial cytochrome b reductase

Gene names

Name:	MCR1
ORF Names:	PGUG_05495

Organism

Pichia guilliermondii (Yeast) (Candida guilliermondii) [Complete proteome]

Taxonomic identifier

4929 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May mediate the reduction of outer membrane cytochrome b5 By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	NADH-cytochrome b5 reductase 2		PRO_0000330188
Regions
	Transmembrane	11 – 27	17	Potential
	Domain	45 – 149	105	FAD-binding FR-type
	Nucleotide binding	152 – 187	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5DQE4-1 [UniParc]. Last modified February 10, 2009. Version 2. Checksum: 83D98BB5338749AE	FASTA	294	32,570
	10 20 30 40 50 60 MSLARFTQPR VLLPVVAAAT SIGLVYHYSS LSIQNDTAKT FKGGDEWIDL KLKKSWDVSS 70 80 90 100 110 120 NTRHFVFELK SPEDVSGLVT ASCLMTKFVT AKGNNVIRPY TPVSDVDQKG TIDFVIKKYD 130 140 150 160 170 180 GGKMSTHFHG LKEGDTVSFK GPIVKWKWEP NQFQSIALIG GGTGITPLYQ LLHEITKNPE 190 200 210 220 230 240 DKTKVKLFYG NLTEEDILIK KELDDIAEKH KDQVSITYFV DKASANWKGE TGHIDKEFLQ 250 260 270 280 290 SNLPGPSKDS KVFVCGPPGL YKALSGVKVS PTDQGEVTGV LAELGYTKEN VYKF

References

[1]

"The genome sequence of Pichia guilliermondii ATCC 6260." Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Cuomo C., Kellis M., Rasmussen M.D., Grochow J.A., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., MacCallum I.A. , Rounsley S., Young S.K., LaButti K., Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J., Zeng Q., Kodira C.D., Yandava C., Alvarado L., O'Leary S., Reedy J., Heitman J. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 6260 / CBS 566 / IFO 10279 / JCM 1539 / NRRL Y-324.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH408161 Genomic DNA. Translation: EDK41397.2.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCR1_PICGU

Accession

Primary (citable) accession number: A5DQE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	February 10, 2009
Last modified:	June 16, 2009
This is version 18 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)