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Protein

Histone-lysine N-methyltransferase, H3 lysine-79 specific

Gene

PGUG_04903

Organism
Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].UniRule annotation

Enzyme regulationi

Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3.UniRule annotation

GO - Molecular functioni

  1. histone-lysine N-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulatorUniRule annotation, MethyltransferaseUniRule annotation, Transferase

Keywords - Ligandi

S-adenosyl-L-methionineUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-79 specificUniRule annotation (EC:2.1.1.43UniRule annotation)
Alternative name(s):
Histone H3-K79 methyltransferaseUniRule annotation
Gene namesi
ORF Names:PGUG_04903Imported
OrganismiMeyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii)Imported
Taxonomic identifieri294746 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeMeyerozyma
ProteomesiUP000001997: Unassembled WGS sequence

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

NucleusUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi4929.A5DNQ2.

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.UniRule annotation
Contains 1 DOT1 domain.UniRule annotation

Phylogenomic databases

InParanoidiA5DNQ2.
KOiK11427.
OrthoDBiEOG7KH9VN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025789. DOT1_dom.
IPR013110. DOT1_dom_Pfam.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51569. DOT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5DNQ2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAQIQGVECG VFSNPPNGVG INTYVDMHHT IHKETPEPLT PSDTNSVMSI
60 70 80 90 100
KSIVTPSQDL PEEKQPNGGI SIENKEDLVS ILKDLVQVDL SMTSLRTKID
110 120 130 140 150
VIDGEKIQNI LNTLEEYDPE ATTVEERQSI EKLATSNHTL KEIRQEHPFR
160 170 180 190 200
DDVAVDRLST EFNPFLRRIK FKNNLDRLLY ISQWYTSSEF NAGKKPDGEI
210 220 230 240 250
EDEAPTDGRR LRPRRKTKQV SPEPEKIEEE KPRTKRRRTK ATVKDETPES
260 270 280 290 300
KDSGDKYNPF DPENILEDTF LPLHGRHAFA DSIYIDAPSL PPLVTNVEPK
310 320 330 340 350
LGVSVDTSAS NLITQHTKHY KNLPSSFPTL FITNSDGQEV ANVNNRIRIR
360 370 380 390 400
FLLYPMHCEE YVLAQPKSNQ LDPVDEIIKF FQVNYGLYFS GSTKIRNIIV
410 420 430 440 450
TNYCQKLREA VDDDDLASFV TIIDKWNQLV LHLSPNPSFW SDINPEIRMY
460 470 480 490 500
TKKIEEKFSE SDLKINIFHT EIAKLVAKHQ GDKQDKNQTN ETNDNDHTVI
510 520 530 540 550
PGSNGSVLAG DSISRYKRDF FCQLASNQKI SRYALHQILS RVYARVVSIS
560 570 580 590 600
SNKLRYYKAF TAEVYGELLP CFTSEVLTKV GMKPHHKFYD LGSGVGNTTL
610 620 630 640 650
QAALEFGAAL SGGCELMAHA SKLTLEQSNM VQRNLSVFGL KQLNLEWALS
660 670 680 690 700
QSFADNEQVR RTVIDCDILI VNNYLFDAEL NYKVGKLLYG LRPGSKIVSL
710 720 730 740 750
RNFISPRYKA SFDDTVFDYL QVEKHEMEHN MSVSWTANKV PYYISVVQKE

VCKQYL
Length:756
Mass (Da):86,139
Last modified:July 22, 2008 - v2
Checksum:i66B4EF1B62BEF45F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH408160 Genomic DNA. Translation: EDK40806.2.
RefSeqiXP_001482949.1. XM_001482899.1.

Genome annotation databases

GeneIDi5124947.
KEGGipgu:PGUG_04903.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH408160 Genomic DNA. Translation: EDK40806.2.
RefSeqiXP_001482949.1. XM_001482899.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4929.A5DNQ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi5124947.
KEGGipgu:PGUG_04903.

Phylogenomic databases

InParanoidiA5DNQ2.
KOiK11427.
OrthoDBiEOG7KH9VN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025789. DOT1_dom.
IPR013110. DOT1_dom_Pfam.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51569. DOT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324Imported.

Entry informationi

Entry nameiA5DNQ2_PICGU
AccessioniPrimary (citable) accession number: A5DNQ2
Entry historyi
Integrated into UniProtKB/TrEMBL: June 12, 2007
Last sequence update: July 22, 2008
Last modified: January 7, 2015
This is version 39 of the entry and version 2 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.