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Protein

Nitrilase

Gene

PGUG_04845

Organism
Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Nitrilase that hydrolyzes preferentially 4-cyanopyridine. Is also able to hydrolyze some aliphatic nitriles, such as phenylacetonitrile.1 Publication

Catalytic activityi

A nitrile + 2 H2O = a carboxylate + NH3.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei45Proton acceptorPROSITE-ProRule annotation1
Active sitei125PROSITE-ProRule annotation1
Active sitei165NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrilase1 Publication (EC:3.5.5.11 Publication)
Gene namesi
ORF Names:PGUG_04845
OrganismiMeyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii)
Taxonomic identifieri294746 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeMeyerozyma
Proteomesi
  • UP000001997 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004321791 – 317NitrilaseAdd BLAST317

Interactioni

Protein-protein interaction databases

STRINGi4929.A5DNJ4.

Structurei

3D structure databases

ProteinModelPortaliA5DNJ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 280CN hydrolasePROSITE-ProRule annotationAdd BLAST276

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0805. Eukaryota.
COG0388. LUCA.
InParanoidiA5DNJ4.
OrthoDBiEOG092C351H.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR036526. C-N_Hydrolase_sf.
IPR000132. Nitrilase/CN_hydratase_CS.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.

Sequencei

Sequence statusi: Complete.

A5DNJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAKVKVAVV QAEPVWFNLQ ETVKRVNELI ESAYNKGAEL IAFPEVFVPG
60 70 80 90 100
YPTWIWTNAA DLDRNLMYTK NSLTYDSPEF ISIIETVKKY PIHVVLGFSE
110 120 130 140 150
KDQGSLYISQ CIIDNTGEIV LKRRKFKPTH VERVIWGDTA DSNMKSVVTL
160 170 180 190 200
NFKEAGPVEV GCLSCWEHMQ PLLYYNSAAQ HEKIHIGSWP ALNDKDLGVY
210 220 230 240 250
CFTKAGFHGL ARAYANQVQS FYLFTSILGQ RIQEALPDVK LSPYFEKGAG
260 270 280 290 300
CGAVFAPDGS QITEDHPDDF DGVIISELDM DKILLQKNLV DIVGHYARPD
310
MVSLSHNRPN TEFVNRK
Length:317
Mass (Da):35,697
Last modified:July 22, 2008 - v2
Checksum:i8B6CDFADDEBEE78B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH408160 Genomic DNA. Translation: EDK40747.2.
RefSeqiXP_001482890.1. XM_001482840.1.

Genome annotation databases

EnsemblFungiiEDK40747; EDK40747; PGUG_04845.
GeneIDi5124984.
KEGGipgu:PGUG_04845.

Similar proteinsi

Entry informationi

Entry nameiNIT_PICGU
AccessioniPrimary (citable) accession number: A5DNJ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 4, 2015
Last sequence update: July 22, 2008
Last modified: October 25, 2017
This is version 43 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families