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A5DHA3 (NTE1_PICGU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysophospholipase NTE1

EC=3.1.1.5
Alternative name(s):
Intracellular phospholipase B
Neuropathy target esterase homolog
Gene names
Name:NTE1
ORF Names:PGUG_02654
OrganismMeyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii) [Complete proteome]
Taxonomic identifier294746 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeMeyerozyma

Protein attributes

Sequence length1438 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium By similarity.

Catalytic activity

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulation

Inhibited by organophosphorus esters By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the NTE family.

Contains 2 cyclic nucleotide-binding domains.

Contains 1 patatin domain.

Ontologies

Keywords
   Biological processLipid degradation
Lipid metabolism
   Cellular componentEndoplasmic reticulum
Membrane
   DomainRepeat
Transmembrane
Transmembrane helix
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlipid catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionlysophospholipase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14381438Lysophospholipase NTE1
PRO_0000295328

Regions

Topological domain1 – 2525Cytoplasmic By similarity
Transmembrane26 – 4621Helical; Potential
Topological domain47 – 6418Lumenal By similarity
Transmembrane65 – 8521Helical; Potential
Topological domain86 – 14381353Cytoplasmic By similarity
Domain1131 – 1295165Patatin
Nucleotide binding590 – 720131cNMP 1
Nucleotide binding707 – 856150cNMP 2
Motif1162 – 11665GXSXG

Sites

Active site11641 By similarity

Sequences

Sequence LengthMass (Da)Tools
A5DHA3 [UniParc].

Last modified February 10, 2009. Version 2.
Checksum: 4961090B281985BB

FASTA1,438160,000
        10         20         30         40         50         60 
MDSDTSSADF HSTETLVSTP KYSYGVLINV ILLVSWTCFR VVNWFLVTLP SILLGMLSKT 

        70         80         90        100        110        120 
FQITLSLSSI LMFVVAVTAI CFLVVRYKYL TRYSRNSAAK EPAKKSFDID DLSKKKKSSS 

       130        140        150        160        170        180 
KTSSNYLDQF LSAIQVFGYL EGPVFHELTR NMTTQKVLPN EVLYLDESLG FSIVVEGTMQ 

       190        200        210        220        230        240 
VYTKVTNLSN SSLNAEDDGL DLRYENDDVL CIGNDKYQLL NEVKSGAPLS SLMSTLDLFT 

       250        260        270        280        290        300 
SNEVPLSPTK FGSYSLTAVT PLELSPNNTG GNSPPPSLAP EIVARPKGTT STTLAIIPYT 

       310        320        330        340        350        360 
AFQRLHKKYP KATSHIVTMV LTRLYKVSMN TIYNYLGLSK DILDCERNLN AETNERLPSY 

       370        380        390        400        410        420 
LSKGIIEKFH KTEESASVEG SPTLPRRSTV NLRALTRQAS SSRYVVLASR SKSTHPGDLL 

       430        440        450        460        470        480 
SSVPLSRRSD YYETQTIPNE SEDSPTIQRS SLRRRASHST SLRKSNSVLE DIRVRGFSNE 

       490        500        510        520        530        540 
REETEETSLR TAIVEQIFTH LGISEDDPTV MNINPTSASS SASSSIVGIS KLNYGSLDSI 

       550        560        570        580        590        600 
NSPRVDPMTS IRSSLASNGN KVYQTINQHT KEADVLQEKP KKVVLPGSRG DDSDFVAVKD 

       610        620        630        640        650        660 
EFARAIQMKY IQPGKTIIEQ DSFHTGIFYV IDGSLDVIQE SNGKSKKIYT VKAGGITGYL 

       670        680        690        700        710        720 
NCLIGMKSLV SVKASEDSSA IVAHIPSHMY SKLIDKFYFL QLPIARRLKR LLSKQFLTID 

       730        740        750        760        770        780 
YALEWCHISA GDILSKEGEP ANGFHIVLSG RFRVVKFKTP FKPVASKAGH DTYDYNDNLI 

       790        800        810        820        830        840 
DESNKKYTSN DVEILGEYGH GESIGEVEVL TASLRKNTLI AVRDSETARI PRALFELLSL 

       850        860        870        880        890        900 
QNPSIMVKVS RIVANRLSKK DSEEILDKPL AMTSSDSPYI SSNFKTITIL PTVSQLPVRE 

       910        920        930        940        950        960 
FADKLVQALK AIGRNVIALD QASTLTHLGK HAFDERLAEL KLSGYFAYLE EKYETIVYVC 

       970        980        990       1000       1010       1020 
DTPVKSNWTS TCISQGDCIL LLADADDEDT AESIGSYERL LIKLKTTART DLCLIHPEKY 

      1030       1040       1050       1060       1070       1080 
VTPGSTSIWL KNRVWVQGHH HIQMEISPTQ TTSVMKPKIP IISELAKKIK DRANPNIKLK 

      1090       1100       1110       1120       1130       1140 
LEEVKGKALA SFVKLNNKIN AHGRMSFNGV SVHKNDFLRL ARILSNEAVG LVLGGGGSRG 

      1150       1160       1170       1180       1190       1200 
ISHVGVVIAL EKHGIPIDLV GGTSIGSFVG GLYAMDYNTV SIYGRAKKFA KRVSSFWRIL 

      1210       1220       1230       1240       1250       1260 
TDLTYPVTSY MTGYEFNRGI WKIFGFTEIE DFWLRYFCNS TNITNSTMDI HETGYSWRFI 

      1270       1280       1290       1300       1310       1320 
RASMSLAGLL PPITYNGSML LDGGYLDNLP VSEMKKKGAK YIIAVDVGSV DDRTPMNYGD 

      1330       1340       1350       1360       1370       1380 
TLSGFWVLLN RWNPFSKHPD VPNMMDIQLR LAYVASVNAL EISKKTPGVM YLRPPIENYA 

      1390       1400       1410       1420       1430 
TLDFAKYDEI YRVGYVYADE LFTSWKSSGK LPDIAGMGDK VRKDMSGEHV SLSRRNSI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH408157 Genomic DNA. Translation: EDK38556.2.
RefSeqXP_001484925.1. XM_001484875.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4929.A5DHA3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5127010.
KEGGpgu:PGUG_02654.

Phylogenomic databases

eggNOGCOG0664.
KOK14676.
OrthoDBEOG70W3NS.

Family and domain databases

Gene3D2.60.120.10. 4 hits.
InterProIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamPF00027. cNMP_binding. 2 hits.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 4 hits.
SSF52151. SSF52151. 1 hit.
PROSITEPS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNTE1_PICGU
AccessionPrimary (citable) accession number: A5DHA3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: February 10, 2009
Last modified: February 19, 2014
This is version 41 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families