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A5DC23 (TRMB_PICGU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:TRM8
ORF Names:PGUG_00828
OrganismMeyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii) [Complete proteome]
Taxonomic identifier294746 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeMeyerozyma

Protein attributes

Sequence length272 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with TRM82 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 272272tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370602

Regions

Region112 – 1132S-adenosyl-L-methionine binding By similarity
Region148 – 1492S-adenosyl-L-methionine binding By similarity
Region246 – 2483S-adenosyl-L-methionine binding By similarity

Sites

Active site1711 By similarity
Binding site891S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1681S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
A5DC23 [UniParc].

Last modified July 22, 2008. Version 2.
Checksum: 87C52BB3B272D3A2

FASTA27231,916
        10         20         30         40         50         60 
MSTDSESKRR AYREEKEGAR KKSVKLAPEA TPESKPDLPR KRYYRQRAHS NPFSDHSLDY 

        70         80         90        100        110        120 
PRNPDAMEWG KLYPEYANTD KKVEIADIGC GYGGLMIGLA KEYPQTMILG MEIRVQVTQY 

       130        140        150        160        170        180 
VEDRIIALRE KHEKDEVNYQ NIAVLRGNAM KFLPNFFHRG QLKKMFFCFP DPHFKQRKHK 

       190        200        210        220        230        240 
ARIITTTLLS EYAYVLREEG VLYTITDVED LHIWMVKHLD DHPLFERLDK EWEKNDKCVS 

       250        260        270 
IMYGSTEEGQ KVARNKGSKF VACFRRVANP EE 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH408155 Genomic DNA. Translation: EDK36730.2.
RefSeqXP_001487451.1. XM_001487401.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4929.A5DC23.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5128595.
KEGGpgu:PGUG_00828.

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OrthoDBEOG708W9T.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_PICGU
AccessionPrimary (citable) accession number: A5DC23
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 22, 2008
Last modified: April 16, 2014
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways