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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei89S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei168S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei171UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:TRM8UniRule annotation
ORF Names:PGUG_00828
OrganismiMeyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii)
Taxonomic identifieri294746 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeMeyerozyma
Proteomesi
  • UP000001997 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003706021 – 272tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST272

Interactioni

Subunit structurei

Forms a complex with TRM82.UniRule annotation

Protein-protein interaction databases

STRINGi4929.A5DC23

Structurei

3D structure databases

SMRiA5DC23
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni112 – 113S-adenosyl-L-methionine bindingUniRule annotation2
Regioni148 – 149S-adenosyl-L-methionine bindingUniRule annotation2
Regioni246 – 248S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115 Eukaryota
COG0220 LUCA
InParanoidiA5DC23
KOiK03439
OMAiIGMEIRV
OrthoDBiEOG092C0GMH

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

A5DC23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDSESKRR AYREEKEGAR KKSVKLAPEA TPESKPDLPR KRYYRQRAHS
60 70 80 90 100
NPFSDHSLDY PRNPDAMEWG KLYPEYANTD KKVEIADIGC GYGGLMIGLA
110 120 130 140 150
KEYPQTMILG MEIRVQVTQY VEDRIIALRE KHEKDEVNYQ NIAVLRGNAM
160 170 180 190 200
KFLPNFFHRG QLKKMFFCFP DPHFKQRKHK ARIITTTLLS EYAYVLREEG
210 220 230 240 250
VLYTITDVED LHIWMVKHLD DHPLFERLDK EWEKNDKCVS IMYGSTEEGQ
260 270
KVARNKGSKF VACFRRVANP EE
Length:272
Mass (Da):31,916
Last modified:July 22, 2008 - v2
Checksum:i87C52BB3B272D3A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH408155 Genomic DNA Translation: EDK36730.2
RefSeqiXP_001487451.1, XM_001487401.1

Genome annotation databases

EnsemblFungiiEDK36730; EDK36730; PGUG_00828
GeneIDi5128595
KEGGipgu:PGUG_00828

Entry informationi

Entry nameiTRMB_PICGU
AccessioniPrimary (citable) accession number: A5DC23
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 22, 2008
Last modified: March 28, 2018
This is version 66 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health