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A5DC23

- TRMB_PICGU

UniProt

A5DC23 - TRMB_PICGU

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei168 – 1681S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei171 – 1711UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:TRM8UniRule annotation
ORF Names:PGUG_00828
OrganismiMeyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii)
Taxonomic identifieri294746 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeMeyerozyma
ProteomesiUP000001997: Unassembled WGS sequence

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 272272tRNA (guanine-N(7)-)-methyltransferasePRO_0000370602Add
BLAST

Interactioni

Subunit structurei

Forms a complex with TRM82.UniRule annotation

Protein-protein interaction databases

STRINGi4929.A5DC23.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni112 – 1132S-adenosyl-L-methionine bindingUniRule annotation
Regioni148 – 1492S-adenosyl-L-methionine bindingUniRule annotation
Regioni246 – 2483S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
InParanoidiA5DC23.
KOiK03439.
OrthoDBiEOG708W9T.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5DC23-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTDSESKRR AYREEKEGAR KKSVKLAPEA TPESKPDLPR KRYYRQRAHS
60 70 80 90 100
NPFSDHSLDY PRNPDAMEWG KLYPEYANTD KKVEIADIGC GYGGLMIGLA
110 120 130 140 150
KEYPQTMILG MEIRVQVTQY VEDRIIALRE KHEKDEVNYQ NIAVLRGNAM
160 170 180 190 200
KFLPNFFHRG QLKKMFFCFP DPHFKQRKHK ARIITTTLLS EYAYVLREEG
210 220 230 240 250
VLYTITDVED LHIWMVKHLD DHPLFERLDK EWEKNDKCVS IMYGSTEEGQ
260 270
KVARNKGSKF VACFRRVANP EE
Length:272
Mass (Da):31,916
Last modified:July 22, 2008 - v2
Checksum:i87C52BB3B272D3A2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH408155 Genomic DNA. Translation: EDK36730.2.
RefSeqiXP_001487451.1. XM_001487401.1.

Genome annotation databases

GeneIDi5128595.
KEGGipgu:PGUG_00828.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH408155 Genomic DNA. Translation: EDK36730.2 .
RefSeqi XP_001487451.1. XM_001487401.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 4929.A5DC23.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5128595.
KEGGi pgu:PGUG_00828.

Phylogenomic databases

eggNOGi COG0220.
InParanoidi A5DC23.
KOi K03439.
OrthoDBi EOG708W9T.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324.

Entry informationi

Entry nameiTRMB_PICGU
AccessioniPrimary (citable) accession number: A5DC23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 22, 2008
Last modified: October 29, 2014
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3