A5DC23 (TRMB_PICGU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	TRM8
ORF Names:	PGUG_00828

Organism

Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii) [Complete proteome]

Taxonomic identifier

294746 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Meyerozyma › Meyerozyma guilliermondii

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subunit structure	Forms a complex with TRM82 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370602
Regions
	Region	112 – 113	2	S-adenosyl-L-methionine binding By similarity
	Region	148 – 149	2	S-adenosyl-L-methionine binding By similarity
	Region	246 – 248	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	171	1	By similarity
	Binding site	89	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	168	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5DC23 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 87C52BB3B272D3A2	FASTA	272	31,916
	10 20 30 40 50 60 MSTDSESKRR AYREEKEGAR KKSVKLAPEA TPESKPDLPR KRYYRQRAHS NPFSDHSLDY 70 80 90 100 110 120 PRNPDAMEWG KLYPEYANTD KKVEIADIGC GYGGLMIGLA KEYPQTMILG MEIRVQVTQY 130 140 150 160 170 180 VEDRIIALRE KHEKDEVNYQ NIAVLRGNAM KFLPNFFHRG QLKKMFFCFP DPHFKQRKHK 190 200 210 220 230 240 ARIITTTLLS EYAYVLREEG VLYTITDVED LHIWMVKHLD DHPLFERLDK EWEKNDKCVS 250 260 270 IMYGSTEEGQ KVARNKGSKF VACFRRVANP EE

References

[1]

"Evolution of pathogenicity and sexual reproduction in eight Candida genomes." Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S., Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I., Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C., Fitzpatrick D.A., de Groot P.W.J. , Harris D., Hoyer L.L., Hube B., Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M., Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F., Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D., Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T., Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C., Birren B.W., Kellis M., Cuomo C.A. Nature 459:657-662(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH408155 Genomic DNA. Translation: EDK36730.2.
RefSeq	XP_001487451.1. XM_001487401.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	4929.A5DC23.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5128595.
KEGG	pgu:PGUG_00828.
Phylogenomic databases
eggNOG	COG0220.
KO	K03439.
OrthoDB	EOG4M3DJF.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_PICGU

Accession

Primary (citable) accession number: A5DC23

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	July 22, 2008
Last modified:	April 3, 2013
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program