A5DB51 (RISA_PICGU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin synthase
Short name=RS
EC=2.5.1.9

Gene names

Name:	RIB5
Synonyms:	RIB7
ORF Names:	PGUG_00506

Organism

Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii) [Complete proteome]

Taxonomic identifier

294746 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Meyerozyma › Meyerozyma guilliermondii

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.
Induction	Repressed by iron. Ref.1
Sequence similarities	Contains 2 lumazine-binding repeats.
Sequence caution	The sequence EDK36408.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Domain	Repeat
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	oxidoreductase activity Inferred from electronic annotation. Source: InterPro riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Riboflavin synthase		PRO_0000295039
Regions
	Repeat	1 – 105	105	Lumazine-binding 1
	Repeat	106 – 205	100	Lumazine-binding 2
	Region	89 – 93	5	Lumazine binding Probable
	Region	189 – 193	5	Lumazine binding Probable

Sequences

	Sequence		Length	Mass (Da)
	A5DB51 [UniParc]. Last modified February 10, 2009. Version 3. Checksum: 64DA06FEFB2D59B2	FASTA	239	26,044
	10 20 30 40 50 60 MFTGLVEHIG TVLQVTEKDT TASGGDGVSM VIGDCSKILE DVQLGDSICT NGVCLTVTEF 70 80 90 100 110 120 DMARSQFKVG ISPETLRRSD LGELKPGSKV NLERAVKADV RMGGHVVQGH VDTIATIVNR 130 140 150 160 170 180 RGDGNAINFT FKLRDSQYGK YIVEKGFIAI DGTSLTVTDV DHEQSEFSIS MVSYTQEKVI 190 200 210 220 230 MPLKNSGDSV NIEVDLTGKL IEKQIELSLL SYIKDETSPL STLIGKLVEK KVDDVLKRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Positive selection of mutants defective in transcriptional repression of riboflavin synthesis by iron in the flavinogenic yeast Pichia guilliermondii." Boretsky Y.R., Kapustyak K.Y., Fayura L.R., Stasyk O.V., Stenchuk M.M., Bobak Y.P., Drobot L.B., Sibirny A.A. FEMS Yeast Res. 5:829-837(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
[2]	"Evolution of pathogenicity and sexual reproduction in eight Candida genomes." Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S., Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I., Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C., Fitzpatrick D.A., de Groot P.W.J. , Harris D., Hoyer L.L., Hube B., Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M., Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F., Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D., Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T., Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C., Birren B.W., Kellis M., Cuomo C.A. Nature 459:657-662(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF459790 Genomic DNA. Translation: AAL66353.2. AY138984 Genomic DNA. Translation: AAN08875.2. CH408155 Genomic DNA. Translation: EDK36408.2. Different initiation.
RefSeq	XP_001487129.1. XM_001487079.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	4929.A5DB51.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5129626.
KEGG	pgu:PGUG_00506.
Phylogenomic databases
eggNOG	COG0307.
KO	K00793.
OrthoDB	EOG4KSSV0.
Enzyme and pathway databases
UniPathway	UPA00275; UER00405.
Family and domain databases
Gene3D	2.40.30.20. 2 hits.
InterPro	IPR023366. ATPase_F1/A1-cplx_a_su_N. IPR001783. Lumazine-bd. IPR026017. Lumazine-bd_dom. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
PANTHER	PTHR21098. PTHR21098. 1 hit.
Pfam	PF00677. Lum_binding. 2 hits. [Graphical view]
PIRSF	PIRSF000498. Riboflavin_syn_A. 1 hit.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 2 hits.
TIGRFAMs	TIGR00187. ribE. 1 hit.
PROSITE	PS51177. LUMAZINE_BIND. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RISA_PICGU

Accession

Primary (citable) accession number: A5DB51
Secondary accession number(s): Q8X181

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	February 10, 2009
Last modified:	April 3, 2013
This is version 35 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program