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Protein

Ubiquitin carboxyl-terminal hydrolase 12

Gene

USP12

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

Activated by interaction with WDR20 and WDR48 through different allosteric mechanisms.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei48NucleophilePROSITE-ProRule annotation1
Metal bindingi185ZincBy similarity1
Metal bindingi188ZincBy similarity1
Metal bindingi232ZincBy similarity1
Metal bindingi235ZincBy similarity1
Active sitei316Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 12 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 12
Ubiquitin thioesterase 12
Ubiquitin-specific-processing protease 12
Gene namesi
Name:USP12
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 12

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003789901 – 369Ubiquitin carboxyl-terminal hydrolase 12Add BLAST369

Proteomic databases

PaxDbiA5D9H7.

Expressioni

Gene expression databases

BgeeiENSBTAG00000017179.
ExpressionAtlasiA5D9H7. baseline and differential.

Interactioni

Subunit structurei

Interacts with WDR48. Interacts with WDR20. Component of the USP12/WDR20/WDR48 deubiquitinating complex.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022828.

Structurei

3D structure databases

SMRiA5D9H7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 368USPAdd BLAST330

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1864. Eukaryota.
ENOG410XQ81. LUCA.
GeneTreeiENSGT00880000137875.
HOGENOMiHOG000231498.
InParanoidiA5D9H7.
KOiK11842.
OMAiEHNAARE.
OrthoDBiEOG091G0FC5.
TreeFamiTF314144.

Family and domain databases

InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.

Sequencei

Sequence statusi: Complete.

A5D9H7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEILMTVSKL ASICTMGANA SALEKEIGPE QFPVNEHYFG LVNFGNTCYC
60 70 80 90 100
NSVLQALYFC RPFREKVLAY KSQPRKKESL LTCLADLFHS IATQKKKVGV
110 120 130 140 150
IPPKKFITRL RKENELFDNY MQQDAHEFLN YLLNTIADIL QEERKQEKQN
160 170 180 190 200
GRLPNGNIDS ENNSTPDPTW VHEIFQGTLT NETRCLTCET ISSKDEDFLD
210 220 230 240 250
LSVDVEQNTS ITHCLRGFSN TETLCSEYKY YCEECRSKQE AHKRMKVKKL
260 270 280 290 300
PMILALHLKR FKYMDQLHRY TKLSYRVVFP LELRLFNTSG DATNPDRMYD
310 320 330 340 350
LVAVVVHCGS GPNRGHYIAI VKSHDFWLLF DDDIVEKIDA QAIEEFYGLT
360
SDISKNSESG YILFYQSRD
Length:369
Mass (Da):42,683
Last modified:June 12, 2007 - v1
Checksum:iE0AC3405433BB353
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT030596 mRNA. Translation: ABQ13036.1.
RefSeqiNP_001091528.1. NM_001098059.1.
UniGeneiBt.24513.

Genome annotation databases

EnsembliENSBTAT00000022828; ENSBTAP00000022828; ENSBTAG00000017179.
GeneIDi525655.
KEGGibta:525655.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiUBP12_BOVIN
AccessioniPrimary (citable) accession number: A5D9H7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: June 12, 2007
Last modified: June 7, 2017
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families