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Protein

Ubiquitin carboxyl-terminal hydrolase 12

Gene

USP12

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2 (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei48 – 481NucleophilePROSITE-ProRule annotation
Active sitei316 – 3161Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. protein deubiquitination Source: UniProtKB
  2. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 12 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 12
Ubiquitin thioesterase 12
Ubiquitin-specific-processing protease 12
Gene namesi
Name:USP12
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 12

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Ubiquitin carboxyl-terminal hydrolase 12PRO_0000378990Add
BLAST

Expressioni

Gene expression databases

ExpressionAtlasiA5D9H7. baseline.

Interactioni

Subunit structurei

Interacts with WDR48.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022828.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 368330USPAdd
BLAST

Sequence similaritiesi

Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00650000093027.
HOGENOMiHOG000231498.
InParanoidiA5D9H7.
KOiK11842.
OMAiSKFASFC.
OrthoDBiEOG7RBZ8G.
TreeFamiTF314144.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5D9H7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEILMTVSKL ASICTMGANA SALEKEIGPE QFPVNEHYFG LVNFGNTCYC
60 70 80 90 100
NSVLQALYFC RPFREKVLAY KSQPRKKESL LTCLADLFHS IATQKKKVGV
110 120 130 140 150
IPPKKFITRL RKENELFDNY MQQDAHEFLN YLLNTIADIL QEERKQEKQN
160 170 180 190 200
GRLPNGNIDS ENNSTPDPTW VHEIFQGTLT NETRCLTCET ISSKDEDFLD
210 220 230 240 250
LSVDVEQNTS ITHCLRGFSN TETLCSEYKY YCEECRSKQE AHKRMKVKKL
260 270 280 290 300
PMILALHLKR FKYMDQLHRY TKLSYRVVFP LELRLFNTSG DATNPDRMYD
310 320 330 340 350
LVAVVVHCGS GPNRGHYIAI VKSHDFWLLF DDDIVEKIDA QAIEEFYGLT
360
SDISKNSESG YILFYQSRD
Length:369
Mass (Da):42,683
Last modified:June 12, 2007 - v1
Checksum:iE0AC3405433BB353
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT030596 mRNA. Translation: ABQ13036.1.
RefSeqiNP_001091528.1. NM_001098059.1.
UniGeneiBt.24513.

Genome annotation databases

EnsembliENSBTAT00000022828; ENSBTAP00000022828; ENSBTAG00000017179.
GeneIDi525655.
KEGGibta:525655.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT030596 mRNA. Translation: ABQ13036.1.
RefSeqiNP_001091528.1. NM_001098059.1.
UniGeneiBt.24513.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022828.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000022828; ENSBTAP00000022828; ENSBTAG00000017179.
GeneIDi525655.
KEGGibta:525655.

Organism-specific databases

CTDi219333.

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00650000093027.
HOGENOMiHOG000231498.
InParanoidiA5D9H7.
KOiK11842.
OMAiSKFASFC.
OrthoDBiEOG7RBZ8G.
TreeFamiTF314144.

Miscellaneous databases

NextBioi20874210.

Gene expression databases

ExpressionAtlasiA5D9H7. baseline.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiUBP12_BOVIN
AccessioniPrimary (citable) accession number: A5D9H7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: June 12, 2007
Last modified: March 4, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.