A5D9H7 (UBP12_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 12
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 12
Ubiquitin thioesterase 12
Ubiquitin-specific-processing protease 12

Gene names

Name:

USP12

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2 By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Interacts with WDR48 By similarity.
Sequence similarities	Belongs to the peptidase C19 family. USP12/USP46 subfamily.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Ubiquitin carboxyl-terminal hydrolase 12		PRO_0000378990
Sites
	Active site	48	1	Nucleophile By similarity
	Active site	316	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5D9H7 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: E0AC3405433BB353	FASTA	369	42,683
	10 20 30 40 50 60 MEILMTVSKL ASICTMGANA SALEKEIGPE QFPVNEHYFG LVNFGNTCYC NSVLQALYFC 70 80 90 100 110 120 RPFREKVLAY KSQPRKKESL LTCLADLFHS IATQKKKVGV IPPKKFITRL RKENELFDNY 130 140 150 160 170 180 MQQDAHEFLN YLLNTIADIL QEERKQEKQN GRLPNGNIDS ENNSTPDPTW VHEIFQGTLT 190 200 210 220 230 240 NETRCLTCET ISSKDEDFLD LSVDVEQNTS ITHCLRGFSN TETLCSEYKY YCEECRSKQE 250 260 270 280 290 300 AHKRMKVKKL PMILALHLKR FKYMDQLHRY TKLSYRVVFP LELRLFNTSG DATNPDRMYD 310 320 330 340 350 360 LVAVVVHCGS GPNRGHYIAI VKSHDFWLLF DDDIVEKIDA QAIEEFYGLT SDISKNSESG YILFYQSRD

References

[1]

"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT030596 mRNA. Translation: ABQ13036.1.
IPI	IPI00852466.
RefSeq	NP_001091528.1. NM_001098059.1.
UniGene	Bt.24513.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000022828; ENSBTAP00000022828; ENSBTAG00000017179.
GeneID	525655.
KEGG	bta:525655.
Organism-specific databases
CTD	219333.
Phylogenomic databases
eggNOG	COG5077.
GeneTree	ENSGT00650000093027.
HOGENOM	HOG000231498.
InParanoid	A5D9H7.
KO	K11842.
OMA	SKFASFC.
OrthoDB	EOG42Z4Q8.
Gene expression databases
ArrayExpress	A5D9H7.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20874210.

Entry information

Entry name

UBP12_BOVIN

Accession

Primary (citable) accession number: A5D9H7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 7, 2009
Last sequence update:	June 12, 2007
Last modified:	March 6, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program