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A5D7R8

- KITH_BOVIN

UniProt

A5D7R8 - KITH_BOVIN

Protein

Thymidine kinase, cytosolic

Gene

TK1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei98 – 981Proton acceptorSequence Analysis
    Binding sitei128 – 1281Substrate; via amide nitrogenBy similarity
    Metal bindingi153 – 1531ZincBy similarity
    Metal bindingi156 – 1561ZincBy similarity
    Binding sitei181 – 1811SubstrateBy similarity
    Metal bindingi185 – 1851ZincBy similarity
    Metal bindingi188 – 1881ZincBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 338ATPCurated
    Nucleotide bindingi58 – 603ATPBy similarity
    Nucleotide bindingi97 – 1004ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. thymidine kinase activity Source: UniProtKB-EC
    3. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. protein homotetramerization Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_210299. Pyrimidine salvage reactions.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase, cytosolic (EC:2.7.1.21)
    Gene namesi
    Name:TK1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 19

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 238237Thymidine kinase, cytosolicPRO_0000322129Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei13 – 131PhosphoserineBy similarity
    Modified residuei235 – 2351PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated on Ser-13 in mitosis.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiA5D7R8.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000034599.

    Structurei

    3D structure databases

    ProteinModelPortaliA5D7R8.
    SMRiA5D7R8. Positions 18-191.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni172 – 1765Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG1435.
    GeneTreeiENSGT00390000011309.
    HOGENOMiHOG000076390.
    HOVERGENiHBG006215.
    InParanoidiA5D7R8.
    KOiK00857.
    OMAiQILQCSP.
    OrthoDBiEOG7MSMPX.
    TreeFamiTF314839.

    Family and domain databases

    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A5D7R8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSCINLPNVL PGSPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQVAQYKC    50
    LVIKYAKDTR YSSLFSTHDR NTMEALPACL LRDVIQDAQR VAVIGIDEGQ 100
    FFPDIVEFCE NMANSGKTVI VAALDGTFQR KAFGTILNLV PLAESVVKLT 150
    AVCMECFREA AYTKRLGVEK EVEVIGGADK YHSVCRLCYF KKASGQPAVL 200
    DSEENKENCP MTLGKPAEAP GVRKLFATHQ IWQCSQAN 238
    Length:238
    Mass (Da):26,377
    Last modified:June 12, 2007 - v1
    Checksum:iB9D720679A18C452
    GO

    Sequence cautioni

    The sequence ABS45010.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT030694 mRNA. Translation: ABS45010.1. Different initiation.
    BC140658 mRNA. Translation: AAI40659.1.
    RefSeqiNP_001091041.1. NM_001097572.1.
    UniGeneiBt.964.

    Genome annotation databases

    EnsembliENSBTAT00000034713; ENSBTAP00000034599; ENSBTAG00000007121.
    GeneIDi504652.
    KEGGibta:504652.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT030694 mRNA. Translation: ABS45010.1 . Different initiation.
    BC140658 mRNA. Translation: AAI40659.1 .
    RefSeqi NP_001091041.1. NM_001097572.1.
    UniGenei Bt.964.

    3D structure databases

    ProteinModelPortali A5D7R8.
    SMRi A5D7R8. Positions 18-191.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000034599.

    Proteomic databases

    PRIDEi A5D7R8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000034713 ; ENSBTAP00000034599 ; ENSBTAG00000007121 .
    GeneIDi 504652.
    KEGGi bta:504652.

    Organism-specific databases

    CTDi 7083.

    Phylogenomic databases

    eggNOGi COG1435.
    GeneTreei ENSGT00390000011309.
    HOGENOMi HOG000076390.
    HOVERGENi HBG006215.
    InParanoidi A5D7R8.
    KOi K00857.
    OMAi QILQCSP.
    OrthoDBi EOG7MSMPX.
    TreeFami TF314839.

    Enzyme and pathway databases

    Reactomei REACT_210299. Pyrimidine salvage reactions.

    Miscellaneous databases

    NextBioi 20866774.

    Family and domain databases

    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal liver.

    Entry informationi

    Entry nameiKITH_BOVIN
    AccessioniPrimary (citable) accession number: A5D7R8
    Secondary accession number(s): A7E3S3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3