A5D7R8 (KITH_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase, cytosolic
EC=2.7.1.21

Gene names

Name:

TK1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Phosphorylated on Ser-13 in mitosis By similarity.
Miscellaneous	Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
Sequence similarities	Belongs to the thymidine kinase family.
Sequence caution	The sequence ABS45010.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 238	237	Thymidine kinase, cytosolic		PRO_0000322129
Regions
	Nucleotide binding	26 – 33	8	ATP Probable
	Nucleotide binding	58 – 60	3	ATP By similarity
	Nucleotide binding	97 – 100	4	ATP By similarity
	Region	172 – 176	5	Substrate binding By similarity
Sites
	Active site	98	1	Proton acceptor Potential
	Metal binding	153	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Metal binding	185	1	Zinc By similarity
	Metal binding	188	1	Zinc By similarity
	Binding site	128	1	Substrate; via amide nitrogen By similarity
	Binding site	181	1	Substrate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	13	1	Phosphoserine By similarity
	Modified residue	235	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5D7R8 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: B9D720679A18C452	FASTA	238	26,377
	10 20 30 40 50 60 MSCINLPNVL PGSPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQVAQYKC LVIKYAKDTR 70 80 90 100 110 120 YSSLFSTHDR NTMEALPACL LRDVIQDAQR VAVIGIDEGQ FFPDIVEFCE NMANSGKTVI 130 140 150 160 170 180 VAALDGTFQR KAFGTILNLV PLAESVVKLT AVCMECFREA AYTKRLGVEK EVEVIGGADK 190 200 210 220 230 YHSVCRLCYF KKASGQPAVL DSEENKENCP MTLGKPAEAP GVRKLFATHQ IWQCSQAN

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT030694 mRNA. Translation: ABS45010.1. Different initiation. BC140658 mRNA. Translation: AAI40659.1.
IPI	IPI00695261.
RefSeq	NP_001091041.1. NM_001097572.1.
UniGene	Bt.964.
3D structure databases
ProteinModelPortal	A5D7R8.
SMR	A5D7R8. Positions 18-191.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000034599.
Proteomic databases
PRIDE	A5D7R8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000034713; ENSBTAP00000034599; ENSBTAG00000007121.
GeneID	504652.
KEGG	bta:504652.
Organism-specific databases
CTD	7083.
Phylogenomic databases
eggNOG	COG1435.
GeneTree	ENSGT00390000011309.
HOGENOM	HOG000076390.
HOVERGEN	HBG006215.
InParanoid	A5D7R8.
KO	K00857.
OMA	KVHAICV.
OrthoDB	EOG4VHK7C.
Family and domain databases
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20866774.

Entry information

Entry name

KITH_BOVIN

Accession

Primary (citable) accession number: A5D7R8
Secondary accession number(s): A7E3S3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 12, 2007
Last modified:	April 3, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program