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A5D7R8 (KITH_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidine kinase, cytosolic

EC=2.7.1.21
Gene names
Name:TK1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length238 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Post-translational modification

Phosphorylated on Ser-13 in mitosis By similarity.

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Sequence similarities

Belongs to the thymidine kinase family.

Sequence caution

The sequence ABS45010.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processDNA synthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionKinase
Transferase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

protein homotetramerization

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

thymidine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

zinc ion binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 238237Thymidine kinase, cytosolic
PRO_0000322129

Regions

Nucleotide binding26 – 338ATP Probable
Nucleotide binding58 – 603ATP By similarity
Nucleotide binding97 – 1004ATP By similarity
Region172 – 1765Substrate binding By similarity

Sites

Active site981Proton acceptor Potential
Metal binding1531Zinc By similarity
Metal binding1561Zinc By similarity
Metal binding1851Zinc By similarity
Metal binding1881Zinc By similarity
Binding site1281Substrate; via amide nitrogen By similarity
Binding site1811Substrate By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue131Phosphoserine By similarity
Modified residue2351Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
A5D7R8 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: B9D720679A18C452

FASTA23826,377
        10         20         30         40         50         60 
MSCINLPNVL PGSPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQVAQYKC LVIKYAKDTR 

        70         80         90        100        110        120 
YSSLFSTHDR NTMEALPACL LRDVIQDAQR VAVIGIDEGQ FFPDIVEFCE NMANSGKTVI 

       130        140        150        160        170        180 
VAALDGTFQR KAFGTILNLV PLAESVVKLT AVCMECFREA AYTKRLGVEK EVEVIGGADK 

       190        200        210        220        230 
YHSVCRLCYF KKASGQPAVL DSEENKENCP MTLGKPAEAP GVRKLFATHQ IWQCSQAN 

« Hide

References

[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Fetal liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BT030694 mRNA. Translation: ABS45010.1. Different initiation.
BC140658 mRNA. Translation: AAI40659.1.
RefSeqNP_001091041.1. NM_001097572.1.
UniGeneBt.964.

3D structure databases

ProteinModelPortalA5D7R8.
SMRA5D7R8. Positions 18-191.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000034599.

Proteomic databases

PRIDEA5D7R8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000034713; ENSBTAP00000034599; ENSBTAG00000007121.
GeneID504652.
KEGGbta:504652.

Organism-specific databases

CTD7083.

Phylogenomic databases

eggNOGCOG1435.
GeneTreeENSGT00390000011309.
HOGENOMHOG000076390.
HOVERGENHBG006215.
InParanoidA5D7R8.
KOK00857.
OMAKVHAICV.
OrthoDBEOG7MSMPX.
TreeFamTF314839.

Family and domain databases

InterProIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERPTHR11441. PTHR11441. 1 hit.
PfamPF00265. TK. 1 hit.
[Graphical view]
PIRSFPIRSF035805. TK_cell. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20866774.

Entry information

Entry nameKITH_BOVIN
AccessionPrimary (citable) accession number: A5D7R8
Secondary accession number(s): A7E3S3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 12, 2007
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families