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A5D7R8

- KITH_BOVIN

UniProt

A5D7R8 - KITH_BOVIN

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Protein
Thymidine kinase, cytosolic
Gene
TK1
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981Proton acceptor Reviewed prediction
Binding sitei128 – 1281Substrate; via amide nitrogen By similarity
Metal bindingi153 – 1531Zinc By similarity
Metal bindingi156 – 1561Zinc By similarity
Binding sitei181 – 1811Substrate By similarity
Metal bindingi185 – 1851Zinc By similarity
Metal bindingi188 – 1881Zinc By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338ATP Inferred
Nucleotide bindingi58 – 603ATP By similarity
Nucleotide bindingi97 – 1004ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. thymidine kinase activity Source: UniProtKB-EC
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein homotetramerization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_210299. Pyrimidine salvage reactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase, cytosolic (EC:2.7.1.21)
Gene namesi
Name:TK1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 19

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 238237Thymidine kinase, cytosolic
PRO_0000322129Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei13 – 131Phosphoserine By similarity
Modified residuei235 – 2351Phosphoserine By similarity

Post-translational modificationi

Phosphorylated on Ser-13 in mitosis By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiA5D7R8.

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000034599.

Structurei

3D structure databases

ProteinModelPortaliA5D7R8.
SMRiA5D7R8. Positions 18-191.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni172 – 1765Substrate binding By similarity

Sequence similaritiesi

Belongs to the thymidine kinase family.

Phylogenomic databases

eggNOGiCOG1435.
GeneTreeiENSGT00390000011309.
HOGENOMiHOG000076390.
HOVERGENiHBG006215.
InParanoidiA5D7R8.
KOiK00857.
OMAiQILQCSP.
OrthoDBiEOG7MSMPX.
TreeFamiTF314839.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5D7R8-1 [UniParc]FASTAAdd to Basket

« Hide

MSCINLPNVL PGSPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQVAQYKC    50
LVIKYAKDTR YSSLFSTHDR NTMEALPACL LRDVIQDAQR VAVIGIDEGQ 100
FFPDIVEFCE NMANSGKTVI VAALDGTFQR KAFGTILNLV PLAESVVKLT 150
AVCMECFREA AYTKRLGVEK EVEVIGGADK YHSVCRLCYF KKASGQPAVL 200
DSEENKENCP MTLGKPAEAP GVRKLFATHQ IWQCSQAN 238
Length:238
Mass (Da):26,377
Last modified:June 12, 2007 - v1
Checksum:iB9D720679A18C452
GO

Sequence cautioni

The sequence ABS45010.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT030694 mRNA. Translation: ABS45010.1. Different initiation.
BC140658 mRNA. Translation: AAI40659.1.
RefSeqiNP_001091041.1. NM_001097572.1.
UniGeneiBt.964.

Genome annotation databases

EnsembliENSBTAT00000034713; ENSBTAP00000034599; ENSBTAG00000007121.
GeneIDi504652.
KEGGibta:504652.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT030694 mRNA. Translation: ABS45010.1 . Different initiation.
BC140658 mRNA. Translation: AAI40659.1 .
RefSeqi NP_001091041.1. NM_001097572.1.
UniGenei Bt.964.

3D structure databases

ProteinModelPortali A5D7R8.
SMRi A5D7R8. Positions 18-191.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000034599.

Proteomic databases

PRIDEi A5D7R8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000034713 ; ENSBTAP00000034599 ; ENSBTAG00000007121 .
GeneIDi 504652.
KEGGi bta:504652.

Organism-specific databases

CTDi 7083.

Phylogenomic databases

eggNOGi COG1435.
GeneTreei ENSGT00390000011309.
HOGENOMi HOG000076390.
HOVERGENi HBG006215.
InParanoidi A5D7R8.
KOi K00857.
OMAi QILQCSP.
OrthoDBi EOG7MSMPX.
TreeFami TF314839.

Enzyme and pathway databases

Reactomei REACT_210299. Pyrimidine salvage reactions.

Miscellaneous databases

NextBioi 20866774.

Family and domain databases

InterProi IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view ]
PANTHERi PTHR11441. PTHR11441. 1 hit.
Pfami PF00265. TK. 1 hit.
[Graphical view ]
PIRSFi PIRSF035805. TK_cell. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal liver.

Entry informationi

Entry nameiKITH_BOVIN
AccessioniPrimary (citable) accession number: A5D7R8
Secondary accession number(s): A7E3S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 12, 2007
Last modified: September 3, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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