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Protein

Thymidine kinase, cytosolic

Gene

TK1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98Proton acceptorSequence analysis1
Binding sitei128Substrate; via amide nitrogenBy similarity1
Metal bindingi153ZincBy similarity1
Metal bindingi156ZincBy similarity1
Binding sitei181SubstrateBy similarity1
Metal bindingi185ZincBy similarity1
Metal bindingi188ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 33ATPCurated8
Nucleotide bindingi58 – 60ATPBy similarity3
Nucleotide bindingi97 – 100ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-BTA-73614. Pyrimidine salvage reactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase, cytosolic (EC:2.7.1.21)
Gene namesi
Name:TK1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003221292 – 238Thymidine kinase, cytosolicAdd BLAST237

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei13PhosphoserineBy similarity1
Modified residuei235PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on Ser-13 in mitosis.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiA5D7R8.
PRIDEiA5D7R8.

Expressioni

Gene expression databases

BgeeiENSBTAG00000007121.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000034599.

Structurei

3D structure databases

ProteinModelPortaliA5D7R8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni172 – 176Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Phylogenomic databases

eggNOGiKOG3125. Eukaryota.
COG1435. LUCA.
GeneTreeiENSGT00390000011309.
HOGENOMiHOG000076390.
HOVERGENiHBG006215.
InParanoidiA5D7R8.
KOiK00857.
OMAiVITGPMY.
OrthoDBiEOG091G0HZR.
TreeFamiTF314839.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5D7R8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCINLPNVL PGSPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQVAQYKC
60 70 80 90 100
LVIKYAKDTR YSSLFSTHDR NTMEALPACL LRDVIQDAQR VAVIGIDEGQ
110 120 130 140 150
FFPDIVEFCE NMANSGKTVI VAALDGTFQR KAFGTILNLV PLAESVVKLT
160 170 180 190 200
AVCMECFREA AYTKRLGVEK EVEVIGGADK YHSVCRLCYF KKASGQPAVL
210 220 230
DSEENKENCP MTLGKPAEAP GVRKLFATHQ IWQCSQAN
Length:238
Mass (Da):26,377
Last modified:June 12, 2007 - v1
Checksum:iB9D720679A18C452
GO

Sequence cautioni

The sequence ABS45010 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT030694 mRNA. Translation: ABS45010.1. Different initiation.
BC140658 mRNA. Translation: AAI40659.1.
RefSeqiNP_001091041.1. NM_001097572.1.
UniGeneiBt.964.

Genome annotation databases

EnsembliENSBTAT00000034713; ENSBTAP00000034599; ENSBTAG00000007121.
GeneIDi504652.
KEGGibta:504652.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT030694 mRNA. Translation: ABS45010.1. Different initiation.
BC140658 mRNA. Translation: AAI40659.1.
RefSeqiNP_001091041.1. NM_001097572.1.
UniGeneiBt.964.

3D structure databases

ProteinModelPortaliA5D7R8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000034599.

Proteomic databases

PaxDbiA5D7R8.
PRIDEiA5D7R8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000034713; ENSBTAP00000034599; ENSBTAG00000007121.
GeneIDi504652.
KEGGibta:504652.

Organism-specific databases

CTDi7083.

Phylogenomic databases

eggNOGiKOG3125. Eukaryota.
COG1435. LUCA.
GeneTreeiENSGT00390000011309.
HOGENOMiHOG000076390.
HOVERGENiHBG006215.
InParanoidiA5D7R8.
KOiK00857.
OMAiVITGPMY.
OrthoDBiEOG091G0HZR.
TreeFamiTF314839.

Enzyme and pathway databases

ReactomeiR-BTA-73614. Pyrimidine salvage reactions.

Gene expression databases

BgeeiENSBTAG00000007121.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKITH_BOVIN
AccessioniPrimary (citable) accession number: A5D7R8
Secondary accession number(s): A7E3S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 12, 2007
Last modified: October 5, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.