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A5D7R8

- KITH_BOVIN

UniProt

A5D7R8 - KITH_BOVIN

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Protein

Thymidine kinase, cytosolic

Gene

TK1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981Proton acceptorSequence Analysis
Binding sitei128 – 1281Substrate; via amide nitrogenBy similarity
Metal bindingi153 – 1531ZincBy similarity
Metal bindingi156 – 1561ZincBy similarity
Binding sitei181 – 1811SubstrateBy similarity
Metal bindingi185 – 1851ZincBy similarity
Metal bindingi188 – 1881ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338ATPCurated
Nucleotide bindingi58 – 603ATPBy similarity
Nucleotide bindingi97 – 1004ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. thymidine kinase activity Source: UniProtKB-EC
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein homotetramerization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_210299. Pyrimidine salvage reactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase, cytosolic (EC:2.7.1.21)
Gene namesi
Name:TK1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 19

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 238237Thymidine kinase, cytosolicPRO_0000322129Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei13 – 131PhosphoserineBy similarity
Modified residuei235 – 2351PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on Ser-13 in mitosis.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiA5D7R8.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000034599.

Structurei

3D structure databases

ProteinModelPortaliA5D7R8.
SMRiA5D7R8. Positions 18-191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni172 – 1765Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Phylogenomic databases

eggNOGiCOG1435.
GeneTreeiENSGT00390000011309.
HOGENOMiHOG000076390.
HOVERGENiHBG006215.
InParanoidiA5D7R8.
KOiK00857.
OMAiQILQCSP.
OrthoDBiEOG7MSMPX.
TreeFamiTF314839.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5D7R8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSCINLPNVL PGSPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQVAQYKC
60 70 80 90 100
LVIKYAKDTR YSSLFSTHDR NTMEALPACL LRDVIQDAQR VAVIGIDEGQ
110 120 130 140 150
FFPDIVEFCE NMANSGKTVI VAALDGTFQR KAFGTILNLV PLAESVVKLT
160 170 180 190 200
AVCMECFREA AYTKRLGVEK EVEVIGGADK YHSVCRLCYF KKASGQPAVL
210 220 230
DSEENKENCP MTLGKPAEAP GVRKLFATHQ IWQCSQAN
Length:238
Mass (Da):26,377
Last modified:June 12, 2007 - v1
Checksum:iB9D720679A18C452
GO

Sequence cautioni

The sequence ABS45010.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT030694 mRNA. Translation: ABS45010.1. Different initiation.
BC140658 mRNA. Translation: AAI40659.1.
RefSeqiNP_001091041.1. NM_001097572.1.
UniGeneiBt.964.

Genome annotation databases

EnsembliENSBTAT00000034713; ENSBTAP00000034599; ENSBTAG00000007121.
GeneIDi504652.
KEGGibta:504652.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT030694 mRNA. Translation: ABS45010.1 . Different initiation.
BC140658 mRNA. Translation: AAI40659.1 .
RefSeqi NP_001091041.1. NM_001097572.1.
UniGenei Bt.964.

3D structure databases

ProteinModelPortali A5D7R8.
SMRi A5D7R8. Positions 18-191.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000034599.

Proteomic databases

PRIDEi A5D7R8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000034713 ; ENSBTAP00000034599 ; ENSBTAG00000007121 .
GeneIDi 504652.
KEGGi bta:504652.

Organism-specific databases

CTDi 7083.

Phylogenomic databases

eggNOGi COG1435.
GeneTreei ENSGT00390000011309.
HOGENOMi HOG000076390.
HOVERGENi HBG006215.
InParanoidi A5D7R8.
KOi K00857.
OMAi QILQCSP.
OrthoDBi EOG7MSMPX.
TreeFami TF314839.

Enzyme and pathway databases

Reactomei REACT_210299. Pyrimidine salvage reactions.

Miscellaneous databases

NextBioi 20866774.

Family and domain databases

InterProi IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view ]
PANTHERi PTHR11441. PTHR11441. 1 hit.
Pfami PF00265. TK. 1 hit.
[Graphical view ]
PIRSFi PIRSF035805. TK_cell. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal liver.

Entry informationi

Entry nameiKITH_BOVIN
AccessioniPrimary (citable) accession number: A5D7R8
Secondary accession number(s): A7E3S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 12, 2007
Last modified: October 29, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3