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A5D7P0

- MPIP3_BOVIN

UniProt

A5D7P0 - MPIP3_BOVIN

Protein

M-phase inducer phosphatase 3

Gene

CDC25C

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activate its kinase activity By similarity.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei381 – 3811By similarity

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. G2/M transition of mitotic cell cycle Source: Ensembl
    2. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_215433. Cyclin B2 mediated events.
    REACT_219578. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_220261. Polo-like kinase mediated events.
    REACT_224007. Cyclin A/B1 associated events during G2/M transition.
    REACT_227740. Activation of ATR in response to replication stress.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    M-phase inducer phosphatase 3 (EC:3.1.3.48)
    Alternative name(s):
    Dual specificity phosphatase Cdc25C
    Gene namesi
    Name:CDC25C
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 7

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 477476M-phase inducer phosphatase 3PRO_0000365162Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei38 – 381PhosphoserineBy similarity
    Modified residuei48 – 481Phosphothreonine; by CDK1By similarity
    Modified residuei58 – 581PhosphoserineBy similarity
    Modified residuei62 – 621PhosphoserineBy similarity
    Modified residuei65 – 651PhosphoserineBy similarity
    Modified residuei68 – 681Phosphothreonine; by CDK1By similarity
    Modified residuei123 – 1231Phosphoserine; by CDK1By similarity
    Modified residuei130 – 1301PhosphoserineBy similarity
    Modified residuei131 – 1311Phosphothreonine; by CDK1By similarity
    Modified residuei169 – 1691Phosphoserine; by CDK1By similarity
    Modified residuei192 – 1921Phosphoserine; by PLK3By similarity
    Modified residuei199 – 1991Phosphoserine; by PLK3By similarity
    Modified residuei218 – 2181Phosphoserine; by CDK1By similarity
    Modified residuei220 – 2201Phosphoserine; by CHEK1, CHEK2, BRSK1 and MAPK14By similarity
    Modified residuei476 – 4761PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by CHEK1 and MAPK14 at Ser-220. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-192 promotes nuclear translocation. Ser-199 is a minor phosphorylation site By similarity. Phosphorylation by CDK1 occurs at G2 and G2-M transition and leads to increased activity By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Expressioni

    Developmental stagei

    Expressed predominantly in G2 phase.

    Interactioni

    Subunit structurei

    Interacts with MAPK14 and 14-3-3 proteins. When phosphorylated on Ser-130 and/or Thr-131, interacts with PLK1.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000006968.

    Structurei

    3D structure databases

    ProteinModelPortaliA5D7P0.
    SMRiA5D7P0. Positions 286-445.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini325 – 432108RhodanesePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MPI phosphatase family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5105.
    GeneTreeiENSGT00390000018747.
    HOGENOMiHOG000082672.
    HOVERGENiHBG052501.
    InParanoidiA5D7P0.
    KOiK05867.
    OMAiTVSLCDI.
    OrthoDBiEOG7288R1.
    TreeFamiTF101056.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view]
    PfamiPF06617. M-inducer_phosp. 2 hits.
    PF00581. Rhodanese. 1 hit.
    [Graphical view]
    PRINTSiPR00716. MPIPHPHTASE.
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A5D7P0-1 [UniParc]FASTAAdd to Basket

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    MSAEFFSSKR EEGSLASGPS FRSNQRKILN LLLERDASFS ISSDLPTTPV    50
    EKKLFGDSAN LSILSGGTPK RCLDLSNLSS GEMSATQLTA SADLDETGHL 100
    ESTGPEQVRL AGMNYRQHLI KCSPAQLFCS TPNALEHGRR KKDAICGSSA 150
    NKENDNGNLV ENEMKHLGSP ITTVSKLHKN PELAEDQAEE ISDELMEFSL 200
    EDQEKAKPPL NWSSLYRSSS LPDSLNSPSL KQVVKFKDST IPDKLKKKYC 250
    SNQKELGKGL GLKKMVSLCD INMTQMLEED SNQGPLIGDF SKVCALPTVS 300
    GKHQDLKYVN PETVAALLSG EFQGLIEKFY IIDCRYPYEY LGGHIQGALN 350
    LHSQEELYNF FLKKPIVPWD NQKRIVIVFH CEFSSERGPR MCRSLREEDR 400
    TLNQYPALYY PELYILKGGY RDFFPEYMEL CEPQSYCPMH HQDHKAELLR 450
    CRNQSKAWEG ERQLQEQIAL LVKDVSP 477
    Length:477
    Mass (Da):53,794
    Last modified:June 12, 2007 - v1
    Checksum:iBC73134038B99915
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC140630 mRNA. Translation: AAI40631.1.
    RefSeqiNP_001091465.1. NM_001097996.2.
    XP_005209468.1. XM_005209411.1.
    XP_005209469.1. XM_005209412.1.
    XP_005209470.1. XM_005209413.1.
    XP_005209471.1. XM_005209414.1.
    XP_005209472.1. XM_005209415.1.
    XP_005209473.1. XM_005209416.1.
    XP_005209474.1. XM_005209417.1.
    UniGeneiBt.43933.

    Genome annotation databases

    EnsembliENSBTAT00000006968; ENSBTAP00000006968; ENSBTAG00000005293.
    GeneIDi507731.
    KEGGibta:507731.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC140630 mRNA. Translation: AAI40631.1 .
    RefSeqi NP_001091465.1. NM_001097996.2.
    XP_005209468.1. XM_005209411.1.
    XP_005209469.1. XM_005209412.1.
    XP_005209470.1. XM_005209413.1.
    XP_005209471.1. XM_005209414.1.
    XP_005209472.1. XM_005209415.1.
    XP_005209473.1. XM_005209416.1.
    XP_005209474.1. XM_005209417.1.
    UniGenei Bt.43933.

    3D structure databases

    ProteinModelPortali A5D7P0.
    SMRi A5D7P0. Positions 286-445.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000006968.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000006968 ; ENSBTAP00000006968 ; ENSBTAG00000005293 .
    GeneIDi 507731.
    KEGGi bta:507731.

    Organism-specific databases

    CTDi 995.

    Phylogenomic databases

    eggNOGi COG5105.
    GeneTreei ENSGT00390000018747.
    HOGENOMi HOG000082672.
    HOVERGENi HBG052501.
    InParanoidi A5D7P0.
    KOi K05867.
    OMAi TVSLCDI.
    OrthoDBi EOG7288R1.
    TreeFami TF101056.

    Enzyme and pathway databases

    Reactomei REACT_215433. Cyclin B2 mediated events.
    REACT_219578. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_220261. Polo-like kinase mediated events.
    REACT_224007. Cyclin A/B1 associated events during G2/M transition.
    REACT_227740. Activation of ATR in response to replication stress.

    Miscellaneous databases

    NextBioi 20868200.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    InterProi IPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view ]
    Pfami PF06617. M-inducer_phosp. 2 hits.
    PF00581. Rhodanese. 1 hit.
    [Graphical view ]
    PRINTSi PR00716. MPIPHPHTASE.
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Thymus.

    Entry informationi

    Entry nameiMPIP3_BOVIN
    AccessioniPrimary (citable) accession number: A5D7P0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 3, 2009
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3