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Protein

M-phase inducer phosphatase 3

Gene

CDC25C

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activate its kinase activity (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei381 – 3811By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_288697. Activation of ATR in response to replication stress.
REACT_302171. Cyclin A/B1 associated events during G2/M transition.
REACT_322110. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_339589. Polo-like kinase mediated events.
REACT_350533. Cyclin B2 mediated events.
REACT_358115. RHO GTPases activate PKNs.

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase 3 (EC:3.1.3.48)
Alternative name(s):
Dual specificity phosphatase Cdc25C
Gene namesi
Name:CDC25C
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 7

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 477476M-phase inducer phosphatase 3PRO_0000365162Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei38 – 381PhosphoserineBy similarity
Modified residuei48 – 481Phosphothreonine; by CDK1By similarity
Modified residuei58 – 581PhosphoserineBy similarity
Modified residuei62 – 621PhosphoserineBy similarity
Modified residuei65 – 651PhosphoserineBy similarity
Modified residuei68 – 681Phosphothreonine; by CDK1By similarity
Modified residuei123 – 1231Phosphoserine; by CDK1By similarity
Modified residuei130 – 1301PhosphoserineBy similarity
Modified residuei131 – 1311PhosphothreonineBy similarity
Modified residuei169 – 1691Phosphoserine; by CDK1By similarity
Modified residuei192 – 1921Phosphoserine; by PLK3By similarity
Modified residuei199 – 1991Phosphoserine; by PLK3By similarity
Modified residuei218 – 2181Phosphoserine; by CDK1By similarity
Modified residuei220 – 2201Phosphoserine; by CHEK1, CHEK2, BRSK1 and MAPK14By similarity
Modified residuei476 – 4761PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by CHEK1 and MAPK14 at Ser-220. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-192 promotes nuclear translocation. Ser-199 is a minor phosphorylation site (By similarity). Phosphorylation by CDK1 occurs at G2 and G2-M transition and leads to increased activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Expressioni

Developmental stagei

Expressed predominantly in G2 phase.

Interactioni

Subunit structurei

Interacts with MAPK14 and 14-3-3 proteins. When phosphorylated on Ser-130 and/or Thr-131, interacts with PLK1.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006968.

Structurei

3D structure databases

ProteinModelPortaliA5D7P0.
SMRiA5D7P0. Positions 286-445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini325 – 432108RhodanesePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated
Contains 1 rhodanese domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5105.
GeneTreeiENSGT00390000018747.
HOGENOMiHOG000082672.
HOVERGENiHBG052501.
InParanoidiA5D7P0.
KOiK05867.
OMAiTVSLCDI.
OrthoDBiEOG7288R1.
TreeFamiTF101056.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF06617. M-inducer_phosp. 2 hits.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5D7P0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAEFFSSKR EEGSLASGPS FRSNQRKILN LLLERDASFS ISSDLPTTPV
60 70 80 90 100
EKKLFGDSAN LSILSGGTPK RCLDLSNLSS GEMSATQLTA SADLDETGHL
110 120 130 140 150
ESTGPEQVRL AGMNYRQHLI KCSPAQLFCS TPNALEHGRR KKDAICGSSA
160 170 180 190 200
NKENDNGNLV ENEMKHLGSP ITTVSKLHKN PELAEDQAEE ISDELMEFSL
210 220 230 240 250
EDQEKAKPPL NWSSLYRSSS LPDSLNSPSL KQVVKFKDST IPDKLKKKYC
260 270 280 290 300
SNQKELGKGL GLKKMVSLCD INMTQMLEED SNQGPLIGDF SKVCALPTVS
310 320 330 340 350
GKHQDLKYVN PETVAALLSG EFQGLIEKFY IIDCRYPYEY LGGHIQGALN
360 370 380 390 400
LHSQEELYNF FLKKPIVPWD NQKRIVIVFH CEFSSERGPR MCRSLREEDR
410 420 430 440 450
TLNQYPALYY PELYILKGGY RDFFPEYMEL CEPQSYCPMH HQDHKAELLR
460 470
CRNQSKAWEG ERQLQEQIAL LVKDVSP
Length:477
Mass (Da):53,794
Last modified:June 12, 2007 - v1
Checksum:iBC73134038B99915
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC140630 mRNA. Translation: AAI40631.1.
RefSeqiNP_001091465.1. NM_001097996.2.
XP_005209469.1. XM_005209412.2.
UniGeneiBt.43933.

Genome annotation databases

EnsembliENSBTAT00000006968; ENSBTAP00000006968; ENSBTAG00000005293.
GeneIDi507731.
KEGGibta:507731.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC140630 mRNA. Translation: AAI40631.1.
RefSeqiNP_001091465.1. NM_001097996.2.
XP_005209469.1. XM_005209412.2.
UniGeneiBt.43933.

3D structure databases

ProteinModelPortaliA5D7P0.
SMRiA5D7P0. Positions 286-445.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006968.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000006968; ENSBTAP00000006968; ENSBTAG00000005293.
GeneIDi507731.
KEGGibta:507731.

Organism-specific databases

CTDi995.

Phylogenomic databases

eggNOGiCOG5105.
GeneTreeiENSGT00390000018747.
HOGENOMiHOG000082672.
HOVERGENiHBG052501.
InParanoidiA5D7P0.
KOiK05867.
OMAiTVSLCDI.
OrthoDBiEOG7288R1.
TreeFamiTF101056.

Enzyme and pathway databases

ReactomeiREACT_288697. Activation of ATR in response to replication stress.
REACT_302171. Cyclin A/B1 associated events during G2/M transition.
REACT_322110. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_339589. Polo-like kinase mediated events.
REACT_350533. Cyclin B2 mediated events.
REACT_358115. RHO GTPases activate PKNs.

Miscellaneous databases

NextBioi20868200.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF06617. M-inducer_phosp. 2 hits.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Thymus.

Entry informationi

Entry nameiMPIP3_BOVIN
AccessioniPrimary (citable) accession number: A5D7P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: June 12, 2007
Last modified: May 27, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.