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Protein

E3 ubiquitin-protein ligase SH3RF1

Gene

SH3RF1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9) (By similarity).By similarity
Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes such as UBE2D1 or UBE2N and then transfers it to substrates. In the absence of an external substrate, it can catalyze self-ubiquitination. Stimulates ubiquitination of potassium channel KCNJ1, enhancing it's dynamin-dependent and clathrin-independent endocytosis (By similarity).By similarity

Pathway:iprotein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri12 – 5342RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase SH3RF1 (EC:6.3.2.-)
Alternative name(s):
Plenty of SH3s
Short name:
Protein POSH
SH3 domain-containing RING finger protein 1
Gene namesi
Name:SH3RF1
Synonyms:POSH
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 840840E3 ubiquitin-protein ligase SH3RF1PRO_0000334150Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei304 – 3041PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated at Ser-304 by AKT1 and AKT2. When phosphorylated, it has reduced ability to bind Rac (By similarity).By similarity
Subject to ubiquitination and proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiA5D7F8.

Expressioni

Gene expression databases

ExpressionAtlasiA5D7F8. baseline.

Interactioni

Subunit structurei

Interacts with Rac; in a GTP-dependent manner. Interacts with MAP3K10 and MAP3K11. Interacts with MAPK8IP; this interaction leads to the PJAC complex (POSH-JIP or SH3RF1/MAPK8IP apoptotic complex) with a 1:1 ratio. Interacts with SIAH1. Interacts with HERP1. Probably part of a signaling complex that may contain SH3RF1, MAPK8IP, DLK1, MAP2K4, MAP2K7, MAPK8, MAPK9, AKT1 and AKT2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000001976.

Structurei

3D structure databases

ProteinModelPortaliA5D7F8.
SMRiA5D7F8. Positions 137-191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini134 – 19360SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini196 – 25964SH3 2PROSITE-ProRule annotationAdd
BLAST
Domaini439 – 50062SH3 3PROSITE-ProRule annotationAdd
BLAST
Domaini781 – 84060SH3 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni434 – 537104Interaction with AKT2By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi338 – 3414Poly-Ala
Compositional biasi368 – 45285Pro-richAdd
BLAST
Compositional biasi613 – 767155Pro-richAdd
BLAST

Domaini

The RING finger domain is responsible of ubiquitination and proteasomal degradation.

Sequence similaritiesi

Belongs to the SH3RF family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 4 SH3 domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri12 – 5342RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, SH3 domain, Zinc-finger

Phylogenomic databases

eggNOGiNOG314495.
HOGENOMiHOG000045564.
HOVERGENiHBG069552.
InParanoidiA5D7F8.
KOiK12171.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR028502. POSH1.
IPR001452. SH3_domain.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR10661:SF8. PTHR10661:SF8. 1 hit.
PfamiPF14604. SH3_9. 4 hits.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00184. RING. 1 hit.
SM00326. SH3. 4 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 4 hits.
PROSITEiPS50002. SH3. 4 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5D7F8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDESALLDLL ECPVCLERLD ASAKVLPCQH TFCKRCLLGI VGSRNELRCP
60 70 80 90 100
ECRTLVGSGV EQLPSNILLV RLLDGIKQRP WKPGPVGGSG TNGTSALRAQ
110 120 130 140 150
SSAVVTCSPK DGPSSQGGPQ PRAQAWSPPV RGIPQLPCAK ALYNYEGKEP
160 170 180 190 200
GDLKFSKGDI IVLRRQVDEN WYHGEVGGVH GFFPTNFVQI IKPLPQPPPQ
210 220 230 240 250
CKALYDFEVK DKEADKDCLP FAKDDVLTVI RRVDENWAEG MLADKIGIFP
260 270 280 290 300
ISYVEFNSAA KQLIEWDQPP GPGVAAGEGA LATTPSSTTT KQPDGKKNTK
310 320 330 340 350
KRHSFTSLSM ASKASQAAQQ RHSMEISPPV LISSSNPAAA ARIGELAGLS
360 370 380 390 400
CSAPSQVHIS TTGLIVTPPP SSPVTTGPSF TFPAEAPYPA ALATLNPPLP
410 420 430 440 450
PPPLQAATPT GTAVAAAAGM GPRPTAGPTD QTTHPRPQPR PSVYVAIYPY
460 470 480 490 500
TPRKEDELEL RKGEMFLVFE RCQDGWFKGT SMHTSKIGVF PGNYVAPVTR
510 520 530 540 550
AVTSASQGKV PMLTTGPASR GGVLANPPST GGPAQKPPGN GVAGGPGVPT
560 570 580 590 600
AVVSAAHVQT SPQAKVLLHA SGQMTVNQAR SAARTVSAHS QERPTAAVTP
610 620 630 640 650
IQVQSTPGQS HHPLVSPQPP APLGPPAHAA ASGLGRVGGP LACATAPASI
660 670 680 690 700
PAASLEPEPS SRPATLLPGT PTSPDSGSAA RPDKDGKKEK KGLLKLLSGA
710 720 730 740 750
STKRKPRGSP PASPTLDAEL GAELSCGPPG PPCACPGPCD GDTMAPGPQR
760 770 780 790 800
RASSLDSAPV APPPRQPCSS LGPAASEVRP AVCERHRVVV SYPPQSEAEL
810 820 830 840
ELKEGDIVFV HKKREDGWFK GTLQRNGKTG LFPGSFVENI
Length:840
Mass (Da):87,592
Last modified:June 12, 2007 - v1
Checksum:i3455AB763557285D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC140539 mRNA. Translation: AAI40540.1.
RefSeqiNP_001091534.1. NM_001098065.1.
UniGeneiBt.44357.

Genome annotation databases

GeneIDi528315.
KEGGibta:528315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC140539 mRNA. Translation: AAI40540.1.
RefSeqiNP_001091534.1. NM_001098065.1.
UniGeneiBt.44357.

3D structure databases

ProteinModelPortaliA5D7F8.
SMRiA5D7F8. Positions 137-191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000001976.

Proteomic databases

PRIDEiA5D7F8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi528315.
KEGGibta:528315.

Organism-specific databases

CTDi57630.

Phylogenomic databases

eggNOGiNOG314495.
HOGENOMiHOG000045564.
HOVERGENiHBG069552.
InParanoidiA5D7F8.
KOiK12171.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

NextBioi20874791.

Gene expression databases

ExpressionAtlasiA5D7F8. baseline.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR028502. POSH1.
IPR001452. SH3_domain.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR10661:SF8. PTHR10661:SF8. 1 hit.
PfamiPF14604. SH3_9. 4 hits.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00184. RING. 1 hit.
SM00326. SH3. 4 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 4 hits.
PROSITEiPS50002. SH3. 4 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.

Entry informationi

Entry nameiSH3R1_BOVIN
AccessioniPrimary (citable) accession number: A5D7F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: March 4, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.