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A5D7F8

- SH3R1_BOVIN

UniProt

A5D7F8 - SH3R1_BOVIN

Protein

E3 ubiquitin-protein ligase SH3RF1

Gene

SH3RF1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9) By similarity.By similarity
    Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes such as UBE2D1 or UBE2N and then transfers it to substrates. In the absence of an external substrate, it can catalyze self-ubiquitination. Stimulates ubiquitination of potassium channel KCNJ1, enhancing it's dynamin-dependent and clathrin-independent endocytosis By similarity.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri12 – 5342RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. negative regulation of apoptotic process Source: Ensembl
    2. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
    3. negative regulation of extrinsic apoptotic signaling pathway Source: Ensembl
    4. protein ubiquitination Source: UniProtKB-UniPathway
    5. regulation of JNK cascade Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase SH3RF1 (EC:6.3.2.-)
    Alternative name(s):
    Plenty of SH3s
    Short name:
    Protein POSH
    SH3 domain-containing RING finger protein 1
    Gene namesi
    Name:SH3RF1
    Synonyms:POSH
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Cytoplasmperinuclear region By similarity. Cell projectionlamellipodium By similarity. Golgi apparatustrans-Golgi network By similarity
    Note: Colocalizes, with AKT2, in lamellipodia. Colocalizes, with HERP1, in trans-Golgi network.By similarity

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. Golgi apparatus Source: UniProtKB-SubCell
    3. lamellipodium Source: UniProtKB-SubCell
    4. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell projection, Cytoplasm, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 840840E3 ubiquitin-protein ligase SH3RF1PRO_0000334150Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei304 – 3041PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated at Ser-304 by AKT1 and AKT2. When phosphorylated, it has reduced ability to bind Rac By similarity.By similarity
    Subject to ubiquitination and proteasomal degradation.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiA5D7F8.

    Interactioni

    Subunit structurei

    Interacts with Rac; in a GTP-dependent manner. Interacts with MAP3K10 and MAP3K11. Interacts with MAPK8IP; this interaction leads to the PJAC complex (POSH-JIP or SH3RF1/MAPK8IP apoptotic complex) with a 1:1 ratio. Interacts with SIAH1. Interacts with HERP1. Probably part of a signaling complex that may contain SH3RF1, MAPK8IP, DLK1, MAP2K4, MAP2K7, MAPK8, MAPK9, AKT1 and AKT2 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000001976.

    Structurei

    3D structure databases

    ProteinModelPortaliA5D7F8.
    SMRiA5D7F8. Positions 137-191.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini134 – 19360SH3 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini196 – 25964SH3 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini439 – 50062SH3 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini781 – 84060SH3 4PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni434 – 537104Interaction with AKT2By similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi338 – 3414Poly-Ala
    Compositional biasi368 – 45285Pro-richAdd
    BLAST
    Compositional biasi613 – 767155Pro-richAdd
    BLAST

    Domaini

    The RING finger domain is responsible of ubiquitination and proteasomal degradation.

    Sequence similaritiesi

    Belongs to the SH3RF family.Curated
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
    Contains 4 SH3 domains.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri12 – 5342RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, SH3 domain, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG314495.
    HOGENOMiHOG000045564.
    HOVERGENiHBG069552.
    InParanoidiA5D7F8.
    KOiK12171.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR028502. POSH1.
    IPR001452. SH3_domain.
    IPR018957. Znf_C3HC4_RING-type.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view]
    PANTHERiPTHR10661:SF8. PTHR10661:SF8. 1 hit.
    PfamiPF14604. SH3_9. 4 hits.
    PF00097. zf-C3HC4. 1 hit.
    [Graphical view]
    PRINTSiPR00452. SH3DOMAIN.
    SMARTiSM00184. RING. 1 hit.
    SM00326. SH3. 4 hits.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 4 hits.
    PROSITEiPS50002. SH3. 4 hits.
    PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5D7F8-1 [UniParc]FASTAAdd to Basket

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    MDESALLDLL ECPVCLERLD ASAKVLPCQH TFCKRCLLGI VGSRNELRCP    50
    ECRTLVGSGV EQLPSNILLV RLLDGIKQRP WKPGPVGGSG TNGTSALRAQ 100
    SSAVVTCSPK DGPSSQGGPQ PRAQAWSPPV RGIPQLPCAK ALYNYEGKEP 150
    GDLKFSKGDI IVLRRQVDEN WYHGEVGGVH GFFPTNFVQI IKPLPQPPPQ 200
    CKALYDFEVK DKEADKDCLP FAKDDVLTVI RRVDENWAEG MLADKIGIFP 250
    ISYVEFNSAA KQLIEWDQPP GPGVAAGEGA LATTPSSTTT KQPDGKKNTK 300
    KRHSFTSLSM ASKASQAAQQ RHSMEISPPV LISSSNPAAA ARIGELAGLS 350
    CSAPSQVHIS TTGLIVTPPP SSPVTTGPSF TFPAEAPYPA ALATLNPPLP 400
    PPPLQAATPT GTAVAAAAGM GPRPTAGPTD QTTHPRPQPR PSVYVAIYPY 450
    TPRKEDELEL RKGEMFLVFE RCQDGWFKGT SMHTSKIGVF PGNYVAPVTR 500
    AVTSASQGKV PMLTTGPASR GGVLANPPST GGPAQKPPGN GVAGGPGVPT 550
    AVVSAAHVQT SPQAKVLLHA SGQMTVNQAR SAARTVSAHS QERPTAAVTP 600
    IQVQSTPGQS HHPLVSPQPP APLGPPAHAA ASGLGRVGGP LACATAPASI 650
    PAASLEPEPS SRPATLLPGT PTSPDSGSAA RPDKDGKKEK KGLLKLLSGA 700
    STKRKPRGSP PASPTLDAEL GAELSCGPPG PPCACPGPCD GDTMAPGPQR 750
    RASSLDSAPV APPPRQPCSS LGPAASEVRP AVCERHRVVV SYPPQSEAEL 800
    ELKEGDIVFV HKKREDGWFK GTLQRNGKTG LFPGSFVENI 840
    Length:840
    Mass (Da):87,592
    Last modified:June 12, 2007 - v1
    Checksum:i3455AB763557285D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC140539 mRNA. Translation: AAI40540.1.
    RefSeqiNP_001091534.1. NM_001098065.1.
    UniGeneiBt.44357.

    Genome annotation databases

    GeneIDi528315.
    KEGGibta:528315.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC140539 mRNA. Translation: AAI40540.1 .
    RefSeqi NP_001091534.1. NM_001098065.1.
    UniGenei Bt.44357.

    3D structure databases

    ProteinModelPortali A5D7F8.
    SMRi A5D7F8. Positions 137-191.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000001976.

    Proteomic databases

    PRIDEi A5D7F8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 528315.
    KEGGi bta:528315.

    Organism-specific databases

    CTDi 57630.

    Phylogenomic databases

    eggNOGi NOG314495.
    HOGENOMi HOG000045564.
    HOVERGENi HBG069552.
    InParanoidi A5D7F8.
    KOi K12171.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    NextBioi 20874791.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR028502. POSH1.
    IPR001452. SH3_domain.
    IPR018957. Znf_C3HC4_RING-type.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view ]
    PANTHERi PTHR10661:SF8. PTHR10661:SF8. 1 hit.
    Pfami PF14604. SH3_9. 4 hits.
    PF00097. zf-C3HC4. 1 hit.
    [Graphical view ]
    PRINTSi PR00452. SH3DOMAIN.
    SMARTi SM00184. RING. 1 hit.
    SM00326. SH3. 4 hits.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 4 hits.
    PROSITEi PS50002. SH3. 4 hits.
    PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Hypothalamus.

    Entry informationi

    Entry nameiSH3R1_BOVIN
    AccessioniPrimary (citable) accession number: A5D7F8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3