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A5D7F8

- SH3R1_BOVIN

UniProt

A5D7F8 - SH3R1_BOVIN

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Protein

E3 ubiquitin-protein ligase SH3RF1

Gene
SH3RF1, POSH
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9) By similarity.
Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes such as UBE2D1 or UBE2N and then transfers it to substrates. In the absence of an external substrate, it can catalyze self-ubiquitination. Stimulates ubiquitination of potassium channel KCNJ1, enhancing it's dynamin-dependent and clathrin-independent endocytosis By similarity.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri12 – 5342RING-typeAdd
BLAST

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. negative regulation of apoptotic process Source: Ensembl
  2. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  3. negative regulation of extrinsic apoptotic signaling pathway Source: Ensembl
  4. protein ubiquitination Source: UniProtKB-UniPathway
  5. regulation of JNK cascade Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase SH3RF1 (EC:6.3.2.-)
Alternative name(s):
Plenty of SH3s
Short name:
Protein POSH
SH3 domain-containing RING finger protein 1
Gene namesi
Name:SH3RF1
Synonyms:POSH
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Cytoplasmperinuclear region By similarity. Cell projectionlamellipodium By similarity. Golgi apparatustrans-Golgi network By similarity
Note: Colocalizes, with AKT2, in lamellipodia By similarity. Colocalizes, with HERP1, in trans-Golgi network By similarity.

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. Golgi apparatus Source: UniProtKB-SubCell
  3. lamellipodium Source: UniProtKB-SubCell
  4. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 840840E3 ubiquitin-protein ligase SH3RF1PRO_0000334150Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei304 – 3041Phosphoserine By similarity

Post-translational modificationi

Phosphorylated at Ser-304 by AKT1 and AKT2. When phosphorylated, it has reduced ability to bind Rac By similarity.
Subject to ubiquitination and proteasomal degradation By similarity.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiA5D7F8.

Interactioni

Subunit structurei

Interacts with Rac; in a GTP-dependent manner. Interacts with MAP3K10 and MAP3K11. Interacts with MAPK8IP; this interaction leads to the PJAC complex (POSH-JIP or SH3RF1/MAPK8IP apoptotic complex) with a 1:1 ratio. Interacts with SIAH1. Interacts with HERP1. Probably part of a signaling complex that may contain SH3RF1, MAPK8IP, DLK1, MAP2K4, MAP2K7, MAPK8, MAPK9, AKT1 and AKT2 By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000001976.

Structurei

3D structure databases

ProteinModelPortaliA5D7F8.
SMRiA5D7F8. Positions 137-191.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini134 – 19360SH3 1Add
BLAST
Domaini196 – 25964SH3 2Add
BLAST
Domaini439 – 50062SH3 3Add
BLAST
Domaini781 – 84060SH3 4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni434 – 537104Interaction with AKT2 By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi338 – 3414Poly-Ala
Compositional biasi368 – 45285Pro-richAdd
BLAST
Compositional biasi613 – 767155Pro-richAdd
BLAST

Domaini

The RING finger domain is responsible of ubiquitination and proteasomal degradation.

Sequence similaritiesi

Belongs to the SH3RF family.
Contains 4 SH3 domains.

Keywords - Domaini

Repeat, SH3 domain, Zinc-finger

Phylogenomic databases

eggNOGiNOG314495.
HOGENOMiHOG000045564.
HOVERGENiHBG069552.
InParanoidiA5D7F8.
KOiK12171.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR028502. POSH1.
IPR001452. SH3_domain.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR10661:SF8. PTHR10661:SF8. 1 hit.
PfamiPF14604. SH3_9. 4 hits.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00184. RING. 1 hit.
SM00326. SH3. 4 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 4 hits.
PROSITEiPS50002. SH3. 4 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5D7F8-1 [UniParc]FASTAAdd to Basket

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MDESALLDLL ECPVCLERLD ASAKVLPCQH TFCKRCLLGI VGSRNELRCP    50
ECRTLVGSGV EQLPSNILLV RLLDGIKQRP WKPGPVGGSG TNGTSALRAQ 100
SSAVVTCSPK DGPSSQGGPQ PRAQAWSPPV RGIPQLPCAK ALYNYEGKEP 150
GDLKFSKGDI IVLRRQVDEN WYHGEVGGVH GFFPTNFVQI IKPLPQPPPQ 200
CKALYDFEVK DKEADKDCLP FAKDDVLTVI RRVDENWAEG MLADKIGIFP 250
ISYVEFNSAA KQLIEWDQPP GPGVAAGEGA LATTPSSTTT KQPDGKKNTK 300
KRHSFTSLSM ASKASQAAQQ RHSMEISPPV LISSSNPAAA ARIGELAGLS 350
CSAPSQVHIS TTGLIVTPPP SSPVTTGPSF TFPAEAPYPA ALATLNPPLP 400
PPPLQAATPT GTAVAAAAGM GPRPTAGPTD QTTHPRPQPR PSVYVAIYPY 450
TPRKEDELEL RKGEMFLVFE RCQDGWFKGT SMHTSKIGVF PGNYVAPVTR 500
AVTSASQGKV PMLTTGPASR GGVLANPPST GGPAQKPPGN GVAGGPGVPT 550
AVVSAAHVQT SPQAKVLLHA SGQMTVNQAR SAARTVSAHS QERPTAAVTP 600
IQVQSTPGQS HHPLVSPQPP APLGPPAHAA ASGLGRVGGP LACATAPASI 650
PAASLEPEPS SRPATLLPGT PTSPDSGSAA RPDKDGKKEK KGLLKLLSGA 700
STKRKPRGSP PASPTLDAEL GAELSCGPPG PPCACPGPCD GDTMAPGPQR 750
RASSLDSAPV APPPRQPCSS LGPAASEVRP AVCERHRVVV SYPPQSEAEL 800
ELKEGDIVFV HKKREDGWFK GTLQRNGKTG LFPGSFVENI 840
Length:840
Mass (Da):87,592
Last modified:June 12, 2007 - v1
Checksum:i3455AB763557285D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC140539 mRNA. Translation: AAI40540.1.
RefSeqiNP_001091534.1. NM_001098065.1.
UniGeneiBt.44357.

Genome annotation databases

GeneIDi528315.
KEGGibta:528315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC140539 mRNA. Translation: AAI40540.1 .
RefSeqi NP_001091534.1. NM_001098065.1.
UniGenei Bt.44357.

3D structure databases

ProteinModelPortali A5D7F8.
SMRi A5D7F8. Positions 137-191.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000001976.

Proteomic databases

PRIDEi A5D7F8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 528315.
KEGGi bta:528315.

Organism-specific databases

CTDi 57630.

Phylogenomic databases

eggNOGi NOG314495.
HOGENOMi HOG000045564.
HOVERGENi HBG069552.
InParanoidi A5D7F8.
KOi K12171.

Enzyme and pathway databases

UniPathwayi UPA00143 .

Miscellaneous databases

NextBioi 20874791.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR028502. POSH1.
IPR001452. SH3_domain.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view ]
PANTHERi PTHR10661:SF8. PTHR10661:SF8. 1 hit.
Pfami PF14604. SH3_9. 4 hits.
PF00097. zf-C3HC4. 1 hit.
[Graphical view ]
PRINTSi PR00452. SH3DOMAIN.
SMARTi SM00184. RING. 1 hit.
SM00326. SH3. 4 hits.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 4 hits.
PROSITEi PS50002. SH3. 4 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.

Entry informationi

Entry nameiSH3R1_BOVIN
AccessioniPrimary (citable) accession number: A5D7F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: June 11, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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