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A5D7F8 (SH3R1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase SH3RF1

EC=6.3.2.-
Alternative name(s):
Plenty of SH3s
Short name=Protein POSH
SH3 domain-containing RING finger protein 1
Gene names
Name:SH3RF1
Synonyms:POSH
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length840 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9) By similarity.

Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes such as UBE2D1 or UBE2N and then transfers it to substrates. In the absence of an external substrate, it can catalyze self-ubiquitination. Stimulates ubiquitination of potassium channel KCNJ1, enhancing it's dynamin-dependent and clathrin-independent endocytosis By similarity.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with Rac; in a GTP-dependent manner. Interacts with MAP3K10 and MAP3K11. Interacts with MAPK8IP; this interaction leads to the PJAC complex (POSH-JIP or SH3RF1/MAPK8IP apoptotic complex) with a 1:1 ratio. Interacts with SIAH1. Interacts with HERP1. Probably part of a signaling complex that may contain SH3RF1, MAPK8IP, DLK1, MAP2K4, MAP2K7, MAPK8, MAPK9, AKT1 and AKT2 By similarity.

Subcellular location

Cytoplasmperinuclear region By similarity. Cell projectionlamellipodium By similarity. Golgi apparatustrans-Golgi network By similarity. Note: Colocalizes, with AKT2, in lamellipodia By similarity. Colocalizes, with HERP1, in trans-Golgi network By similarity.

Domain

The RING finger domain is responsible of ubiquitination and proteasomal degradation.

Post-translational modification

Phosphorylated at Ser-304 by AKT1 and AKT2. When phosphorylated, it has reduced ability to bind Rac By similarity.

Subject to ubiquitination and proteasomal degradation By similarity.

Sequence similarities

Belongs to the SH3RF family.

Contains 1 RING-type zinc finger.

Contains 4 SH3 domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 840840E3 ubiquitin-protein ligase SH3RF1
PRO_0000334150

Regions

Domain134 – 19360SH3 1
Domain196 – 25964SH3 2
Domain439 – 50062SH3 3
Domain781 – 84060SH3 4
Zinc finger12 – 5342RING-type
Region434 – 537104Interaction with AKT2 By similarity
Compositional bias338 – 3414Poly-Ala
Compositional bias368 – 45285Pro-rich
Compositional bias613 – 767155Pro-rich

Amino acid modifications

Modified residue3041Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
A5D7F8 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 3455AB763557285D

FASTA84087,592
        10         20         30         40         50         60 
MDESALLDLL ECPVCLERLD ASAKVLPCQH TFCKRCLLGI VGSRNELRCP ECRTLVGSGV 

        70         80         90        100        110        120 
EQLPSNILLV RLLDGIKQRP WKPGPVGGSG TNGTSALRAQ SSAVVTCSPK DGPSSQGGPQ 

       130        140        150        160        170        180 
PRAQAWSPPV RGIPQLPCAK ALYNYEGKEP GDLKFSKGDI IVLRRQVDEN WYHGEVGGVH 

       190        200        210        220        230        240 
GFFPTNFVQI IKPLPQPPPQ CKALYDFEVK DKEADKDCLP FAKDDVLTVI RRVDENWAEG 

       250        260        270        280        290        300 
MLADKIGIFP ISYVEFNSAA KQLIEWDQPP GPGVAAGEGA LATTPSSTTT KQPDGKKNTK 

       310        320        330        340        350        360 
KRHSFTSLSM ASKASQAAQQ RHSMEISPPV LISSSNPAAA ARIGELAGLS CSAPSQVHIS 

       370        380        390        400        410        420 
TTGLIVTPPP SSPVTTGPSF TFPAEAPYPA ALATLNPPLP PPPLQAATPT GTAVAAAAGM 

       430        440        450        460        470        480 
GPRPTAGPTD QTTHPRPQPR PSVYVAIYPY TPRKEDELEL RKGEMFLVFE RCQDGWFKGT 

       490        500        510        520        530        540 
SMHTSKIGVF PGNYVAPVTR AVTSASQGKV PMLTTGPASR GGVLANPPST GGPAQKPPGN 

       550        560        570        580        590        600 
GVAGGPGVPT AVVSAAHVQT SPQAKVLLHA SGQMTVNQAR SAARTVSAHS QERPTAAVTP 

       610        620        630        640        650        660 
IQVQSTPGQS HHPLVSPQPP APLGPPAHAA ASGLGRVGGP LACATAPASI PAASLEPEPS 

       670        680        690        700        710        720 
SRPATLLPGT PTSPDSGSAA RPDKDGKKEK KGLLKLLSGA STKRKPRGSP PASPTLDAEL 

       730        740        750        760        770        780 
GAELSCGPPG PPCACPGPCD GDTMAPGPQR RASSLDSAPV APPPRQPCSS LGPAASEVRP 

       790        800        810        820        830        840 
AVCERHRVVV SYPPQSEAEL ELKEGDIVFV HKKREDGWFK GTLQRNGKTG LFPGSFVENI 

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References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Hypothalamus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC140539 mRNA. Translation: AAI40540.1.
RefSeqNP_001091534.1. NM_001098065.1.
UniGeneBt.44357.

3D structure databases

ProteinModelPortalA5D7F8.
SMRA5D7F8. Positions 137-191.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000001976.

Proteomic databases

PRIDEA5D7F8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID528315.
KEGGbta:528315.

Organism-specific databases

CTD57630.

Phylogenomic databases

eggNOGNOG314495.
HOGENOMHOG000045564.
HOVERGENHBG069552.
InParanoidA5D7F8.
KOK12171.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR028502. POSH1.
IPR001452. SH3_domain.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERPTHR10661:SF8. PTHR10661:SF8. 1 hit.
PfamPF00097. zf-C3HC4. 1 hit.
[Graphical view]
PRINTSPR00452. SH3DOMAIN.
SMARTSM00184. RING. 1 hit.
SM00326. SH3. 4 hits.
[Graphical view]
SUPFAMSSF50044. SSF50044. 4 hits.
PROSITEPS50002. SH3. 4 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20874791.

Entry information

Entry nameSH3R1_BOVIN
AccessionPrimary (citable) accession number: A5D7F8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: February 19, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways