ACTN4

Functionⁱ

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA.By similarity

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Calcium bindingⁱ	778 – 789	1PROSITE-ProRule annotationAdd BLAST		12
Calcium bindingⁱ	819 – 830	2PROSITE-ProRule annotationAdd BLAST		12

GO - Molecular functionⁱ

actin filament binding Source: Ensembl
calcium ion binding Source: InterPro
chromatin DNA binding Source: Ensembl
ion channel binding Source: Ensembl
ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
nucleoside binding Source: Ensembl
protein homodimerization activity Source: Ensembl
retinoic acid receptor binding Source: Ensembl
RNA binding Source: Ensembl
RNA polymerase II regulatory region sequence-specific DNA binding Source: Ensembl

Complete GO annotation...

GO - Biological processⁱ

actin filament bundle assembly Source: Ensembl
bicellular tight junction assembly Source: Ensembl
negative regulation of cellular component movement Source: Ensembl
negative regulation of substrate adhesion-dependent cell spreading Source: Ensembl
peroxisome proliferator activated receptor signaling pathway Source: UniProtKB
positive regulation of cell migration Source: Ensembl
positive regulation of NIK/NF-kappaB signaling Source: Ensembl
positive regulation of pinocytosis Source: Ensembl
protein localization to bicellular tight junction Source: Ensembl
protein transport Source: UniProtKB-KW
retinoic acid receptor signaling pathway Source: UniProtKB
vesicle transport along actin filament Source: Ensembl

Complete GO annotation...

Keywordsⁱ

Molecular function	Actin-binding
Biological process	Protein transport, Transport
Ligand	Calcium, Metal-binding

Enzyme and pathway databases

Reactomeⁱ	R-BTA-114608. Platelet degranulation.

Reactomeⁱ

R-BTA-114608. Platelet degranulation.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Alpha-actinin-4Curated Alternative name(s): Non-muscle alpha-actinin 4Curated
Gene namesⁱ	Name:ACTN4Curated
Organismⁱ	Bos taurus (Bovine)
Taxonomic identifierⁱ	9913 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos
Proteomesⁱ	UP000009136 Componentⁱ: Chromosome 18

Subcellular locationⁱ

Nucleus
By similarity
Manual assertion inferred from sequence similarity toⁱ
- UniProtKB:O43707 (ACTN4_HUMAN)
Cytoplasm
By similarity
Manual assertion inferred from sequence similarity toⁱ
- UniProtKB:O43707 (ACTN4_HUMAN)
Cell junction
By similarity
Manual assertion inferred from sequence similarity toⁱ
- UniProtKB:O43707 (ACTN4_HUMAN)

Note:

Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers.By similarity

GO - Cellular componentⁱ

brush border Source: Ensembl
cell-cell junction Source: Ensembl
cortical cytoskeleton Source: Ensembl
cytoplasm Source: UniProtKB
cytosol Source: Ensembl
extracellular exosome Source: Ensembl
extracellular space Source: Ensembl
focal adhesion Source: Ensembl
intracellular ribonucleoprotein complex Source: UniProtKB
nuclear body Source: Ensembl
nucleus Source: UniProtKB
perinuclear region of cytoplasm Source: Ensembl
protein complex Source: Ensembl
pseudopodium Source: Ensembl
stress fiber Source: Ensembl
Z disc Source: Ensembl

Complete GO annotation...

Keywords - Cellular componentⁱ

Cell junction, Cytoplasm, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000312775	1 – 911	Alpha-actinin-4Add BLAST		911

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	114	N6-acetyllysineBy similarity	1
Modified residueⁱ	249	PhosphothreonineBy similarity	1
Modified residueⁱ	592	N6-acetyllysineBy similarity	1
Modified residueⁱ	625	N6-acetyllysineBy similarity	1
Modified residueⁱ	779	N6-acetyllysineBy similarity	1
Modified residueⁱ	859	N6-acetyllysineBy similarity	1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

PaxDbⁱ	A5D7D1.
PeptideAtlas More...PeptideAtlasⁱ	A5D7D1.
PRIDEⁱ	A5D7D1.

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSBTAG00000011215.

Bgeeⁱ

ENSBTAG00000011215.

Interactionⁱ

Subunit structureⁱ

Homodimer; antiparallel. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Binds TRIM3 at the N-terminus. Interacts with BAIAP1 and PDLIM2. Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1. Interacts with MICALL2 (preferentially in opened conformation); stimulated by RAB13 activation. Interacts with PPARG and RARA. Binds to VCL; this interaction triggers VCL conformational changes (By similarity).By similarity

GO - Molecular functionⁱ

actin filament binding Source: Ensembl
ion channel binding Source: Ensembl
protein homodimerization activity Source: Ensembl
retinoic acid receptor binding Source: Ensembl

Complete GO annotation...

Protein-protein interaction databases

IntActⁱ	A5D7D1. 2 interactors.
STRINGⁱ	9913.ENSBTAP00000014894.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	A5D7D1.
SMRⁱ	A5D7D1.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	1 – 269	Actin-bindingAdd BLAST	269
Domainⁱ	50 – 154	CH 1PROSITE-ProRule annotationAdd BLAST	105
Domainⁱ	163 – 269	CH 2PROSITE-ProRule annotationAdd BLAST	107
Repeatⁱ	293 – 403	Spectrin 1Sequence analysisAdd BLAST	111
Repeatⁱ	413 – 518	Spectrin 2Sequence analysisAdd BLAST	106
Repeatⁱ	528 – 639	Spectrin 3Sequence analysisAdd BLAST	112
Repeatⁱ	649 – 752	Spectrin 4Sequence analysisAdd BLAST	104
Domainⁱ	765 – 800	EF-hand 1PROSITE-ProRule annotationAdd BLAST	36
Domainⁱ	806 – 841	EF-hand 2PROSITE-ProRule annotationAdd BLAST	36

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	12 – 26	Interaction with VCLBy similarityAdd BLAST	15
Regionⁱ	40 – 61	Interaction with VCLBy similarityAdd BLAST	22
Regionⁱ	108 – 126	Interaction with VCLBy similarityAdd BLAST	19
Regionⁱ	177 – 192	Polyphosphoinositide (PIP2)-bindingSequence analysisAdd BLAST	16
Regionⁱ	736 – 911	Mediates interaction with MICALL2By similarityAdd BLAST	176

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	84 – 88	LXXLL motifBy similarity		5

Domainⁱ

Contains one Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif that mediates interaction with nuclear receptors.By similarity

Sequence similaritiesⁱ

Belongs to the alpha-actinin family.Curated

Keywords - Domainⁱ

Repeat

Phylogenomic databases

eggNOGⁱ	KOG0035. Eukaryota. COG5069. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118813.
HOGENOMⁱ	HOG000263418.
HOVERGENⁱ	HBG050453.
InParanoidⁱ	A5D7D1.
KEGG Orthology (KO) More...KOⁱ	K05699.
OMAⁱ	QANIVGP.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G020R.
TreeFamⁱ	TF352676.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00014. CH. 2 hits. cd00051. EFh. 1 hit.
Gene3Dⁱ	1.10.418.10. 2 hits.
InterProⁱ	View protein in InterPro IPR001589. Actinin_actin-bd_CS. IPR001715. CH-domain. IPR011992. EF-hand-dom_pair. IPR014837. EF-hand_Ca_insen. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR018159. Spectrin/alpha-actinin. IPR002017. Spectrin_repeat.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00307. CH. 2 hits. PF08726. EFhand_Ca_insen. 1 hit. PF00435. Spectrin. 4 hits.
SMARTⁱ	View protein in SMART SM00033. CH. 2 hits. SM00054. EFh. 2 hits. SM00150. SPEC. 4 hits.
SUPFAMⁱ	SSF47473. SSF47473. 1 hit. SSF47576. SSF47576. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00019. ACTININ_1. 1 hit. PS00020. ACTININ_2. 1 hit. PS50021. CH. 2 hits. PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits.

Sequenceⁱ

Sequence statusⁱ: Complete.

A5D7D1-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MVDYHAANQS YQYGPSSGSN GAGGGGTMGD YMAQEDDWDR DLLLDPAWEK 
        60         70         80         90        100
QQRKTFTAWC NSHLRKAGTQ IENIDEDFRD GLKLMLLLEV ISGERLPKPE 
       110        120        130        140        150
RGKMRVHKIN NVNKALDFIA SKGVKLVSIG AEEIVDGNAK MTLGMIWTII 
       160        170        180        190        200
LRFAIQDISV EETSAKEGLL LWCQRKTAPY KNVNVQNFHI SWKDGLAFNA 
       210        220        230        240        250
LIHRHRPELI EYDKLRKDDP VTNLNNAFEV AEKYLDIPKM LDAEDIVNTA 
       260        270        280        290        300
RPDEKAIMTY VSSFYHAFSG AQKAETAANR ICKVLAVNQE NEHLMEDYER 
       310        320        330        340        350
LASDLLEWIR RTIPWLEDRV PQKTIQEMQQ KLEDFRDYRR VHKPPKVQEK 
       360        370        380        390        400
CQLEINFNTL QTKLRLSNRP AFMPSEGKMV SDINNGWQHL EQAEKGYEEW 
       410        420        430        440        450
LLNEIRRLER LDHLAEKFRQ KASIHEAWTD GKEAMLKHRD YETATLSDIK 
       460        470        480        490        500
ALIRKHEAFE SDLAAHQDRV EQIAAIAQEL NELDYYDSHN VNTRCQKICD 
       510        520        530        540        550
QWDALGSLTH SRREALEKTE KQLETIDQLH LEYAKRAAPF NNWMESAMED 
       560        570        580        590        600
LQDMFIVHTI EEIEGLISAH DQFKSTLPDA DREREAILAI HKEAQRIAES 
       610        620        630        640        650
NHIKLSGSNP YTTVTPQIIN SKWEKVQQLV PKRDHALLEE QSKQQSNEHL 
       660        670        680        690        700
RRQFASQANI VGPWIQTKME EIGRISIEMN GTLEDQLSHL KQYERSIVDY 
       710        720        730        740        750
KPNLDLLEQQ HQLIQEALIF DNKHTNYTME HIRVGWEQLL TTIARTINEV 
       760        770        780        790        800
ENQILTRDAK GISQEQMQEF RASFNHFDKD HGGALGPEEF KACLISLGYD 
       810        820        830        840        850
VENDRQGDAE FNRIMSVVDP NHSGLVTFQA FIDFMSRETT DTDTADQVIA 
       860        870        880        890        900
SFKVLAGDKN FITAEELRRE LPPDQAEYCI ARMAPYQGPD AVPGALDYKS 
       910 
FSTALYGESD L

Length:911

Mass (Da):104,928

Last modified:June 12, 2007 - v1

Checksum:ⁱ78BBD1E1F8F935D6

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BC140512 mRNA. Translation: AAI40513.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_001091521.1. NM_001098052.1.
UniGeneⁱ	Bt.13633.

Genome annotation databases

Ensemblⁱ	ENSBTAT00000014894; ENSBTAP00000014894; ENSBTAG00000011215.
GeneIDⁱ	522269.
KEGGⁱ	bta:522269.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BC140512 mRNA. Translation: AAI40513.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_001091521.1. NM_001098052.1.
UniGeneⁱ	Bt.13633.

3D structure databases

ProteinModelPortalⁱ	A5D7D1.
SMRⁱ	A5D7D1.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	A5D7D1. 2 interactors.
STRINGⁱ	9913.ENSBTAP00000014894.

Proteomic databases

PaxDbⁱ	A5D7D1.
PeptideAtlas More...PeptideAtlasⁱ	A5D7D1.
PRIDEⁱ	A5D7D1.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSBTAT00000014894; ENSBTAP00000014894; ENSBTAG00000011215.
GeneIDⁱ	522269.
KEGGⁱ	bta:522269.

Organism-specific databases

CTDⁱ	81.

CTDⁱ

81.

Phylogenomic databases

eggNOGⁱ	KOG0035. Eukaryota. COG5069. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118813.
HOGENOMⁱ	HOG000263418.
HOVERGENⁱ	HBG050453.
InParanoidⁱ	A5D7D1.
KEGG Orthology (KO) More...KOⁱ	K05699.
OMAⁱ	QANIVGP.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G020R.
TreeFamⁱ	TF352676.

Enzyme and pathway databases

Reactomeⁱ	R-BTA-114608. Platelet degranulation.

Reactomeⁱ

R-BTA-114608. Platelet degranulation.

Gene expression databases

Bgeeⁱ	ENSBTAG00000011215.

Bgeeⁱ

ENSBTAG00000011215.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00014. CH. 2 hits. cd00051. EFh. 1 hit.
Gene3Dⁱ	1.10.418.10. 2 hits.
InterProⁱ	View protein in InterPro IPR001589. Actinin_actin-bd_CS. IPR001715. CH-domain. IPR011992. EF-hand-dom_pair. IPR014837. EF-hand_Ca_insen. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR018159. Spectrin/alpha-actinin. IPR002017. Spectrin_repeat.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00307. CH. 2 hits. PF08726. EFhand_Ca_insen. 1 hit. PF00435. Spectrin. 4 hits.
SMARTⁱ	View protein in SMART SM00033. CH. 2 hits. SM00054. EFh. 2 hits. SM00150. SPEC. 4 hits.
SUPFAMⁱ	SSF47473. SSF47473. 1 hit. SSF47576. SSF47576. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00019. ACTININ_1. 1 hit. PS00020. ACTININ_2. 1 hit. PS50021. CH. 2 hits. PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ACTN4_BOVIN
Accessionⁱ	A5D7D1Primary (citable) accession number: A5D7D1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
	Last sequence update:	June 12, 2007
	Last modified:	June 7, 2017
	This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - A5D7D1 (ACTN4_BOVIN)

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Alpha-actinin-4

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ