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Protein

Alpha-actinin-4

Gene

ACTN4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi778 – 7891PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi819 – 8302PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processProtein transport, Transport
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-114608. Platelet degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-actinin-4Curated
Alternative name(s):
Non-muscle alpha-actinin 4Curated
Gene namesi
Name:ACTN4Curated
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 18

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003127751 – 911Alpha-actinin-4Add BLAST911

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei114N6-acetyllysineBy similarity1
Modified residuei249PhosphothreonineBy similarity1
Modified residuei592N6-acetyllysineBy similarity1
Modified residuei625N6-acetyllysineBy similarity1
Modified residuei779N6-acetyllysineBy similarity1
Modified residuei859N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiA5D7D1.
PeptideAtlasiA5D7D1.
PRIDEiA5D7D1.

Expressioni

Gene expression databases

BgeeiENSBTAG00000011215.

Interactioni

Subunit structurei

Homodimer; antiparallel. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Binds TRIM3 at the N-terminus. Interacts with BAIAP1 and PDLIM2. Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1. Interacts with MICALL2 (preferentially in opened conformation); stimulated by RAB13 activation. Interacts with PPARG and RARA. Binds to VCL; this interaction triggers VCL conformational changes (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiA5D7D1. 2 interactors.
STRINGi9913.ENSBTAP00000014894.

Structurei

3D structure databases

ProteinModelPortaliA5D7D1.
SMRiA5D7D1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 154Calponin-homology (CH) 1PROSITE-ProRule annotationAdd BLAST105
Domaini163 – 266Calponin-homology (CH) 2PROSITE-ProRule annotationAdd BLAST104
Repeati293 – 403Spectrin 1Sequence analysisAdd BLAST111
Repeati413 – 518Spectrin 2Sequence analysisAdd BLAST106
Repeati528 – 639Spectrin 3Sequence analysisAdd BLAST112
Repeati649 – 752Spectrin 4Sequence analysisAdd BLAST104
Domaini765 – 800EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini806 – 841EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 269Actin-bindingAdd BLAST269
Regioni12 – 26Interaction with VCLBy similarityAdd BLAST15
Regioni40 – 61Interaction with VCLBy similarityAdd BLAST22
Regioni108 – 126Interaction with VCLBy similarityAdd BLAST19
Regioni177 – 192Polyphosphoinositide (PIP2)-bindingSequence analysisAdd BLAST16
Regioni736 – 911Mediates interaction with MICALL2By similarityAdd BLAST176

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi84 – 88LXXLL motifBy similarity5

Domaini

Contains one Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif that mediates interaction with nuclear receptors.By similarity

Sequence similaritiesi

Belongs to the alpha-actinin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0035. Eukaryota.
COG5069. LUCA.
GeneTreeiENSGT00760000118813.
HOGENOMiHOG000263418.
HOVERGENiHBG050453.
InParanoidiA5D7D1.
KOiK05699.
OMAiQANIVGP.
OrthoDBiEOG091G020R.
TreeFamiTF352676.

Family and domain databases

CDDicd00014. CH. 2 hits.
cd00051. EFh. 1 hit.
Gene3Di1.10.418.10. 2 hits.
InterProiView protein in InterPro
IPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR014837. EF-hand_Ca_insen.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
PfamiView protein in Pfam
PF00307. CH. 2 hits.
PF08726. EFhand_Ca_insen. 1 hit.
PF00435. Spectrin. 4 hits.
SMARTiView protein in SMART
SM00033. CH. 2 hits.
SM00054. EFh. 2 hits.
SM00150. SPEC. 4 hits.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiView protein in PROSITE
PS00019. ACTININ_1. 1 hit.
PS00020. ACTININ_2. 1 hit.
PS50021. CH. 2 hits.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.

Sequencei

Sequence statusi: Complete.

A5D7D1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDYHAANQS YQYGPSSGSN GAGGGGTMGD YMAQEDDWDR DLLLDPAWEK
60 70 80 90 100
QQRKTFTAWC NSHLRKAGTQ IENIDEDFRD GLKLMLLLEV ISGERLPKPE
110 120 130 140 150
RGKMRVHKIN NVNKALDFIA SKGVKLVSIG AEEIVDGNAK MTLGMIWTII
160 170 180 190 200
LRFAIQDISV EETSAKEGLL LWCQRKTAPY KNVNVQNFHI SWKDGLAFNA
210 220 230 240 250
LIHRHRPELI EYDKLRKDDP VTNLNNAFEV AEKYLDIPKM LDAEDIVNTA
260 270 280 290 300
RPDEKAIMTY VSSFYHAFSG AQKAETAANR ICKVLAVNQE NEHLMEDYER
310 320 330 340 350
LASDLLEWIR RTIPWLEDRV PQKTIQEMQQ KLEDFRDYRR VHKPPKVQEK
360 370 380 390 400
CQLEINFNTL QTKLRLSNRP AFMPSEGKMV SDINNGWQHL EQAEKGYEEW
410 420 430 440 450
LLNEIRRLER LDHLAEKFRQ KASIHEAWTD GKEAMLKHRD YETATLSDIK
460 470 480 490 500
ALIRKHEAFE SDLAAHQDRV EQIAAIAQEL NELDYYDSHN VNTRCQKICD
510 520 530 540 550
QWDALGSLTH SRREALEKTE KQLETIDQLH LEYAKRAAPF NNWMESAMED
560 570 580 590 600
LQDMFIVHTI EEIEGLISAH DQFKSTLPDA DREREAILAI HKEAQRIAES
610 620 630 640 650
NHIKLSGSNP YTTVTPQIIN SKWEKVQQLV PKRDHALLEE QSKQQSNEHL
660 670 680 690 700
RRQFASQANI VGPWIQTKME EIGRISIEMN GTLEDQLSHL KQYERSIVDY
710 720 730 740 750
KPNLDLLEQQ HQLIQEALIF DNKHTNYTME HIRVGWEQLL TTIARTINEV
760 770 780 790 800
ENQILTRDAK GISQEQMQEF RASFNHFDKD HGGALGPEEF KACLISLGYD
810 820 830 840 850
VENDRQGDAE FNRIMSVVDP NHSGLVTFQA FIDFMSRETT DTDTADQVIA
860 870 880 890 900
SFKVLAGDKN FITAEELRRE LPPDQAEYCI ARMAPYQGPD AVPGALDYKS
910
FSTALYGESD L
Length:911
Mass (Da):104,928
Last modified:June 12, 2007 - v1
Checksum:i78BBD1E1F8F935D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC140512 mRNA. Translation: AAI40513.1.
RefSeqiNP_001091521.1. NM_001098052.1.
UniGeneiBt.13633.

Genome annotation databases

EnsembliENSBTAT00000014894; ENSBTAP00000014894; ENSBTAG00000011215.
GeneIDi522269.
KEGGibta:522269.

Similar proteinsi

Entry informationi

Entry nameiACTN4_BOVIN
AccessioniPrimary (citable) accession number: A5D7D1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: June 12, 2007
Last modified: September 27, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families