UniProtKB - A5D7D1 (ACTN4_BOVIN)
(max 400 entries)x
Your basket is currently empty. i
Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)
Protein
Alpha-actinin-4
Gene
ACTN4
Organism
Bos taurus (Bovine)
Status
Functioni
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 778 – 789 | 1PROSITE-ProRule annotationAdd BLAST | 12 | |
Calcium bindingi | 819 – 830 | 2PROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- actin filament binding Source: Ensembl
- calcium ion binding Source: InterPro
- chromatin DNA binding Source: Ensembl
- ion channel binding Source: Ensembl
- ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
- nucleoside binding Source: Ensembl
- protein homodimerization activity Source: Ensembl
- retinoic acid receptor binding Source: Ensembl
- RNA polymerase II regulatory region sequence-specific DNA binding Source: Ensembl
GO - Biological processi
- actin filament bundle assembly Source: Ensembl
- bicellular tight junction assembly Source: Ensembl
- negative regulation of cellular component movement Source: Ensembl
- negative regulation of substrate adhesion-dependent cell spreading Source: Ensembl
- peroxisome proliferator activated receptor signaling pathway Source: UniProtKB
- positive regulation of cell migration Source: Ensembl
- positive regulation of NIK/NF-kappaB signaling Source: Ensembl
- positive regulation of pinocytosis Source: Ensembl
- protein localization to bicellular tight junction Source: Ensembl
- protein transport Source: UniProtKB-KW
- retinoic acid receptor signaling pathway Source: UniProtKB
- vesicle transport along actin filament Source: Ensembl
Keywordsi
Molecular function | Actin-binding |
Biological process | Protein transport, Transport |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-BTA-114608. Platelet degranulation. |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-actinin-4CuratedAlternative name(s): Non-muscle alpha-actinin 4Curated |
Gene namesi | Name:ACTN4Curated |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:25583. ACTN4. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000312775 | 1 – 911 | Alpha-actinin-4Add BLAST | 911 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 114 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 249 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 592 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 625 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 779 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 859 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | A5D7D1. |
PeptideAtlasi | A5D7D1. |
PRIDEi | A5D7D1. |
Interactioni
Subunit structurei
Homodimer; antiparallel. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Binds TRIM3 at the N-terminus. Interacts with BAIAP1 and PDLIM2. Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1. Interacts with MICALL2 (preferentially in opened conformation); stimulated by RAB13 activation. Interacts with PPARG and RARA. Binds to VCL; this interaction triggers VCL conformational changes (By similarity).By similarity
GO - Molecular functioni
- actin filament binding Source: Ensembl
- ion channel binding Source: Ensembl
- protein homodimerization activity Source: Ensembl
- retinoic acid receptor binding Source: Ensembl
Protein-protein interaction databases
IntActi | A5D7D1. 2 interactors. |
STRINGi | 9913.ENSBTAP00000014894. |
Structurei
3D structure databases
ProteinModelPortali | A5D7D1. |
SMRi | A5D7D1. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 50 – 154 | Calponin-homology (CH) 1PROSITE-ProRule annotationAdd BLAST | 105 | |
Domaini | 163 – 269 | Calponin-homology (CH) 2PROSITE-ProRule annotationAdd BLAST | 107 | |
Repeati | 293 – 403 | Spectrin 1Sequence analysisAdd BLAST | 111 | |
Repeati | 413 – 518 | Spectrin 2Sequence analysisAdd BLAST | 106 | |
Repeati | 528 – 639 | Spectrin 3Sequence analysisAdd BLAST | 112 | |
Repeati | 649 – 752 | Spectrin 4Sequence analysisAdd BLAST | 104 | |
Domaini | 765 – 800 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 806 – 841 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 269 | Actin-bindingAdd BLAST | 269 | |
Regioni | 12 – 26 | Interaction with VCLBy similarityAdd BLAST | 15 | |
Regioni | 40 – 61 | Interaction with VCLBy similarityAdd BLAST | 22 | |
Regioni | 108 – 126 | Interaction with VCLBy similarityAdd BLAST | 19 | |
Regioni | 177 – 192 | Polyphosphoinositide (PIP2)-bindingSequence analysisAdd BLAST | 16 | |
Regioni | 736 – 911 | Mediates interaction with MICALL2By similarityAdd BLAST | 176 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 84 – 88 | LXXLL motifBy similarity | 5 |
Domaini
Contains one Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif that mediates interaction with nuclear receptors.By similarity
Sequence similaritiesi
Belongs to the alpha-actinin family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0035. Eukaryota. COG5069. LUCA. |
GeneTreei | ENSGT00760000118813. |
HOGENOMi | HOG000263418. |
HOVERGENi | HBG050453. |
InParanoidi | A5D7D1. |
KOi | K05699. |
OMAi | QANIVGP. |
OrthoDBi | EOG091G020R. |
TreeFami | TF352676. |
Family and domain databases
CDDi | cd00014. CH. 2 hits. cd00051. EFh. 1 hit. |
Gene3Di | 1.10.418.10. 2 hits. |
InterProi | View protein in InterPro IPR001589. Actinin_actin-bd_CS. IPR029637. ACTN4. IPR001715. CH-domain. IPR036872. CH_dom_sf. IPR011992. EF-hand-dom_pair. IPR014837. EF-hand_Ca_insen. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR018159. Spectrin/alpha-actinin. IPR002017. Spectrin_repeat. |
PANTHERi | PTHR43947:SF7. PTHR43947:SF7. 1 hit. |
Pfami | View protein in Pfam PF00307. CH. 2 hits. PF08726. EFhand_Ca_insen. 1 hit. PF00435. Spectrin. 4 hits. |
SMARTi | View protein in SMART SM00033. CH. 2 hits. SM00054. EFh. 2 hits. SM00150. SPEC. 4 hits. |
SUPFAMi | SSF47473. SSF47473. 1 hit. SSF47576. SSF47576. 1 hit. |
PROSITEi | View protein in PROSITE PS00019. ACTININ_1. 1 hit. PS00020. ACTININ_2. 1 hit. PS50021. CH. 2 hits. PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. |
i Sequence
Sequence statusi: Complete.
A5D7D1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVDYHAANQS YQYGPSSGSN GAGGGGTMGD YMAQEDDWDR DLLLDPAWEK
60 70 80 90 100
QQRKTFTAWC NSHLRKAGTQ IENIDEDFRD GLKLMLLLEV ISGERLPKPE
110 120 130 140 150
RGKMRVHKIN NVNKALDFIA SKGVKLVSIG AEEIVDGNAK MTLGMIWTII
160 170 180 190 200
LRFAIQDISV EETSAKEGLL LWCQRKTAPY KNVNVQNFHI SWKDGLAFNA
210 220 230 240 250
LIHRHRPELI EYDKLRKDDP VTNLNNAFEV AEKYLDIPKM LDAEDIVNTA
260 270 280 290 300
RPDEKAIMTY VSSFYHAFSG AQKAETAANR ICKVLAVNQE NEHLMEDYER
310 320 330 340 350
LASDLLEWIR RTIPWLEDRV PQKTIQEMQQ KLEDFRDYRR VHKPPKVQEK
360 370 380 390 400
CQLEINFNTL QTKLRLSNRP AFMPSEGKMV SDINNGWQHL EQAEKGYEEW
410 420 430 440 450
LLNEIRRLER LDHLAEKFRQ KASIHEAWTD GKEAMLKHRD YETATLSDIK
460 470 480 490 500
ALIRKHEAFE SDLAAHQDRV EQIAAIAQEL NELDYYDSHN VNTRCQKICD
510 520 530 540 550
QWDALGSLTH SRREALEKTE KQLETIDQLH LEYAKRAAPF NNWMESAMED
560 570 580 590 600
LQDMFIVHTI EEIEGLISAH DQFKSTLPDA DREREAILAI HKEAQRIAES
610 620 630 640 650
NHIKLSGSNP YTTVTPQIIN SKWEKVQQLV PKRDHALLEE QSKQQSNEHL
660 670 680 690 700
RRQFASQANI VGPWIQTKME EIGRISIEMN GTLEDQLSHL KQYERSIVDY
710 720 730 740 750
KPNLDLLEQQ HQLIQEALIF DNKHTNYTME HIRVGWEQLL TTIARTINEV
760 770 780 790 800
ENQILTRDAK GISQEQMQEF RASFNHFDKD HGGALGPEEF KACLISLGYD
810 820 830 840 850
VENDRQGDAE FNRIMSVVDP NHSGLVTFQA FIDFMSRETT DTDTADQVIA
860 870 880 890 900
SFKVLAGDKN FITAEELRRE LPPDQAEYCI ARMAPYQGPD AVPGALDYKS
910
FSTALYGESD L
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC140512 mRNA. Translation: AAI40513.1. |
RefSeqi | NP_001091521.1. NM_001098052.1. |
UniGenei | Bt.13633. |
Genome annotation databases
Ensembli | ENSBTAT00000014894; ENSBTAP00000014894; ENSBTAG00000011215. |
GeneIDi | 522269. |
KEGGi | bta:522269. |
Similar proteinsi
Entry informationi
Entry namei | ACTN4_BOVIN | |
Accessioni | A5D7D1Primary (citable) accession number: A5D7D1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 4, 2007 |
Last sequence update: | June 12, 2007 | |
Last modified: | March 28, 2018 | |
This is version 91 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |