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A5D6R3

- PLD3A_DANRE

UniProt

A5D6R3 - PLD3A_DANRE

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3-A

Gene

plcd3a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca2+ from intracellular stores By similarity.By similarity

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei328 – 3281PROSITE-ProRule annotation
    Metal bindingi329 – 3291Calcium 1; catalyticBy similarity
    Metal bindingi358 – 3581Calcium 1; catalyticBy similarity
    Metal bindingi360 – 3601Calcium 1; catalyticBy similarity
    Active sitei373 – 3731PROSITE-ProRule annotation
    Metal bindingi407 – 4071Calcium 1; catalyticBy similarity
    Binding sitei456 – 4561SubstrateBy similarity
    Binding sitei458 – 4581SubstrateBy similarity
    Binding sitei534 – 5341SubstrateBy similarity
    Binding sitei561 – 5611SubstrateBy similarity
    Metal bindingi664 – 6641Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi666 – 6661Calcium 2By similarity
    Metal bindingi690 – 6901Calcium 2By similarity
    Metal bindingi719 – 7191Calcium 3By similarity
    Metal bindingi721 – 7211Calcium 3By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi172 – 183121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi208 – 219122PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. angiogenesis Source: InterPro
    2. intracellular signal transduction Source: InterPro
    3. lipid catabolic process Source: UniProtKB-KW
    4. regulation of cell proliferation Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_202687. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3-A (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C-delta-3-A
    Phospholipase C-delta-3-A
    Short name:
    PLC-delta-3-A
    Gene namesi
    Name:plcd3a
    ORF Names:zgc:158396
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 3

    Organism-specific databases

    ZFINiZDB-GENE-070620-1. plcd3a.

    Subcellular locationi

    Membrane By similarity; Peripheral membrane protein By similarity. Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7847841-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3-APRO_0000306823Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Gene expression databases

    BgeeiA5D6R3.

    Interactioni

    Protein-protein interaction databases

    STRINGi7955.ENSDARP00000093449.

    Structurei

    3D structure databases

    ProteinModelPortaliA5D6R3.
    SMRiA5D6R3. Positions 219-768.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 149112PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini159 – 19436EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini195 – 23036EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini227 – 26236EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini313 – 458146PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini506 – 621116PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini628 – 733106C2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni48 – 7831Substrate bindingBy similarityAdd
    BLAST

    Domaini

    The C2 domain is a Ca2+-dependent membrane-targeting module.By similarity
    The PH domain mediates interaction with the surface membrane by binding to PIP2.By similarity

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 3 EF-hand domains.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG149692.
    GeneTreeiENSGT00740000114979.
    HOGENOMiHOG000006871.
    KOiK05857.
    OrthoDBiEOG7V49XT.
    PhylomeDBiA5D6R3.
    TreeFamiTF313216.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR028406. PLC-delta3.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF33. PTHR10336:SF33. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00169. PH. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00054. EFh. 2 hits.
    SM00233. PH. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS50003. PH_DOMAIN. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5D6R3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLGRKKNPET VQTESKSVES KTHDPLRRLG VLDDEDVLLM LQGSKMMKVR    50
    SQRWRKDRRL KLLEDCVTVW CESSKTSRKS NRQQTFSVTE VECVREGCQS 100
    ECLRRMTDLV PEKNCFTVVF RGGRKSLDLC CHTQEEAERW VRGIRTLKDR 150
    VSNMSQKEKL DHWIRGYLRR ADQNQDGKMS YDEVKHLLQL INIDLNEQYA 200
    RTLFKKCDRS CDGRLDHVEI EEFCREMMRR PELDAVFRHY SGNGCVLTTL 250
    ELRDFLGDQG EDASLVHAKS LIQTFELNDW AQKNLFMTQN GFTMYMLSKE 300
    NDVFNPDHTH VYQDMSKPLA HYYISSSHNT YLTKDQVTSA SSTEPYIRAL 350
    NQGCRCVELD CWDGDKGEPI IYHGHTLTSK VLFKEVIETI AQYAFKASPY 400
    PLILSLENHC SVEQQAIMAQ QLQSILGKKL LAKPLSDLPL KQLPSPEELK 450
    GRILLKGKKL NGLLGKTESW TSFTNSSDEE SVAGGNKKES KKDLARSAST 500
    KLSPELSDLV VYCQSVPFSG FETANQRPPS VITSFSENEA LKLIKDSGKL 550
    FVRNNSRQLS RIYPSAQRLQ SSNFDPQDMW NAGCQMVALN FQTPGEQMDL 600
    NQGRFLPNGR CGYVLKPEFL CDPKSDFDPE NTGGGPGHIP TQLTIRVISA 650
    QQLPKINTDK PNSIVDPQVW VEIHGVSIDK ARAKTQRIDN NGFNPRWDCT 700
    VSFQLQLPEL ALVRFMVEDH DHKSKNDFIG QFTLPFTSLR TGYRHVHLLK 750
    ADGSTLSPAT LFIHVKVVRR GVHIKTVSER IGKA 784
    Length:784
    Mass (Da):89,381
    Last modified:June 12, 2007 - v1
    Checksum:i4078823F379343C6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC139849 mRNA. Translation: AAI39850.1.
    RefSeqiNP_001092893.1. NM_001099423.1.
    UniGeneiDr.50929.

    Genome annotation databases

    EnsembliENSDART00000102674; ENSDARP00000093449; ENSDARG00000052957.
    GeneIDi569040.
    KEGGidre:569040.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC139849 mRNA. Translation: AAI39850.1 .
    RefSeqi NP_001092893.1. NM_001099423.1.
    UniGenei Dr.50929.

    3D structure databases

    ProteinModelPortali A5D6R3.
    SMRi A5D6R3. Positions 219-768.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7955.ENSDARP00000093449.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000102674 ; ENSDARP00000093449 ; ENSDARG00000052957 .
    GeneIDi 569040.
    KEGGi dre:569040.

    Organism-specific databases

    CTDi 569040.
    ZFINi ZDB-GENE-070620-1. plcd3a.

    Phylogenomic databases

    eggNOGi NOG149692.
    GeneTreei ENSGT00740000114979.
    HOGENOMi HOG000006871.
    KOi K05857.
    OrthoDBi EOG7V49XT.
    PhylomeDBi A5D6R3.
    TreeFami TF313216.

    Enzyme and pathway databases

    Reactomei REACT_202687. Synthesis of IP3 and IP4 in the cytosol.

    Miscellaneous databases

    NextBioi 20889471.
    PROi A5D6R3.

    Gene expression databases

    Bgeei A5D6R3.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR028406. PLC-delta3.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF33. PTHR10336:SF33. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00169. PH. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00054. EFh. 2 hits.
    SM00233. PH. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS50003. PH_DOMAIN. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    2. "Duplication of phospholipase C-delta gene family in fish genomes."
      Kim M.S., Seo J.S., Ahn S.J., Kim N.Y., Je J.E., Sung J.H., Lee H.H., Chung J.K.
      Genomics 92:366-371(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE DUPLICATION.

    Entry informationi

    Entry nameiPLD3A_DANRE
    AccessioniPrimary (citable) accession number: A5D6R3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3