SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A5D6R3

- PLD3A_DANRE

UniProt

A5D6R3 - PLD3A_DANRE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3-A
Gene
plcd3a, zgc:158396
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca2+ from intracellular stores By similarity.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei328 – 3281 By similarity
Metal bindingi329 – 3291Calcium 1; catalytic By similarity
Metal bindingi358 – 3581Calcium 1; catalytic By similarity
Metal bindingi360 – 3601Calcium 1; catalytic By similarity
Active sitei373 – 3731 By similarity
Metal bindingi407 – 4071Calcium 1; catalytic By similarity
Binding sitei456 – 4561Substrate By similarity
Binding sitei458 – 4581Substrate By similarity
Binding sitei534 – 5341Substrate By similarity
Binding sitei561 – 5611Substrate By similarity
Metal bindingi664 – 6641Calcium 2; via carbonyl oxygen By similarity
Metal bindingi666 – 6661Calcium 2 By similarity
Metal bindingi690 – 6901Calcium 2 By similarity
Metal bindingi719 – 7191Calcium 3 By similarity
Metal bindingi721 – 7211Calcium 3 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi172 – 183121 Reviewed prediction
Add
BLAST
Calcium bindingi208 – 219122 Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. angiogenesis Source: InterPro
  2. intracellular signal transduction Source: InterPro
  3. lipid catabolic process Source: UniProtKB-KW
  4. regulation of cell proliferation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_202687. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3-A (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-delta-3-A
Phospholipase C-delta-3-A
Short name:
PLC-delta-3-A
Gene namesi
Name:plcd3a
ORF Names:zgc:158396
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 3

Organism-specific databases

ZFINiZDB-GENE-070620-1. plcd3a.

Subcellular locationi

Membrane; Peripheral membrane protein By similarity. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7847841-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3-A
PRO_0000306823Add
BLAST

Keywords - PTMi

Phosphoprotein

Expressioni

Gene expression databases

BgeeiA5D6R3.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000093449.

Structurei

3D structure databases

ProteinModelPortaliA5D6R3.
SMRiA5D6R3. Positions 219-768.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 149112PH
Add
BLAST
Domaini159 – 19436EF-hand 1
Add
BLAST
Domaini195 – 23036EF-hand 2
Add
BLAST
Domaini227 – 26236EF-hand 3
Add
BLAST
Domaini313 – 458146PI-PLC X-box
Add
BLAST
Domaini506 – 621116PI-PLC Y-box
Add
BLAST
Domaini628 – 733106C2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 7831Substrate binding By similarity
Add
BLAST

Domaini

The C2 domain is a Ca2+-dependent membrane-targeting module By similarity.
The PH domain mediates interaction with the surface membrane by binding to PIP2 By similarity.

Sequence similaritiesi

Contains 1 C2 domain.
Contains 3 EF-hand domains.
Contains 1 PH domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00740000114979.
HOGENOMiHOG000006871.
KOiK05857.
OrthoDBiEOG7V49XT.
PhylomeDBiA5D6R3.
TreeFamiTF313216.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR028406. PLC-delta3.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF33. PTHR10336:SF33. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00169. PH. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00233. PH. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5D6R3-1 [UniParc]FASTAAdd to Basket

« Hide

MLGRKKNPET VQTESKSVES KTHDPLRRLG VLDDEDVLLM LQGSKMMKVR    50
SQRWRKDRRL KLLEDCVTVW CESSKTSRKS NRQQTFSVTE VECVREGCQS 100
ECLRRMTDLV PEKNCFTVVF RGGRKSLDLC CHTQEEAERW VRGIRTLKDR 150
VSNMSQKEKL DHWIRGYLRR ADQNQDGKMS YDEVKHLLQL INIDLNEQYA 200
RTLFKKCDRS CDGRLDHVEI EEFCREMMRR PELDAVFRHY SGNGCVLTTL 250
ELRDFLGDQG EDASLVHAKS LIQTFELNDW AQKNLFMTQN GFTMYMLSKE 300
NDVFNPDHTH VYQDMSKPLA HYYISSSHNT YLTKDQVTSA SSTEPYIRAL 350
NQGCRCVELD CWDGDKGEPI IYHGHTLTSK VLFKEVIETI AQYAFKASPY 400
PLILSLENHC SVEQQAIMAQ QLQSILGKKL LAKPLSDLPL KQLPSPEELK 450
GRILLKGKKL NGLLGKTESW TSFTNSSDEE SVAGGNKKES KKDLARSAST 500
KLSPELSDLV VYCQSVPFSG FETANQRPPS VITSFSENEA LKLIKDSGKL 550
FVRNNSRQLS RIYPSAQRLQ SSNFDPQDMW NAGCQMVALN FQTPGEQMDL 600
NQGRFLPNGR CGYVLKPEFL CDPKSDFDPE NTGGGPGHIP TQLTIRVISA 650
QQLPKINTDK PNSIVDPQVW VEIHGVSIDK ARAKTQRIDN NGFNPRWDCT 700
VSFQLQLPEL ALVRFMVEDH DHKSKNDFIG QFTLPFTSLR TGYRHVHLLK 750
ADGSTLSPAT LFIHVKVVRR GVHIKTVSER IGKA 784
Length:784
Mass (Da):89,381
Last modified:June 12, 2007 - v1
Checksum:i4078823F379343C6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC139849 mRNA. Translation: AAI39850.1.
RefSeqiNP_001092893.1. NM_001099423.1.
UniGeneiDr.50929.

Genome annotation databases

EnsembliENSDART00000102674; ENSDARP00000093449; ENSDARG00000052957.
GeneIDi569040.
KEGGidre:569040.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC139849 mRNA. Translation: AAI39850.1 .
RefSeqi NP_001092893.1. NM_001099423.1.
UniGenei Dr.50929.

3D structure databases

ProteinModelPortali A5D6R3.
SMRi A5D6R3. Positions 219-768.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7955.ENSDARP00000093449.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000102674 ; ENSDARP00000093449 ; ENSDARG00000052957 .
GeneIDi 569040.
KEGGi dre:569040.

Organism-specific databases

CTDi 569040.
ZFINi ZDB-GENE-070620-1. plcd3a.

Phylogenomic databases

eggNOGi NOG149692.
GeneTreei ENSGT00740000114979.
HOGENOMi HOG000006871.
KOi K05857.
OrthoDBi EOG7V49XT.
PhylomeDBi A5D6R3.
TreeFami TF313216.

Enzyme and pathway databases

Reactomei REACT_202687. Synthesis of IP3 and IP4 in the cytosol.

Miscellaneous databases

NextBioi 20889471.
PROi A5D6R3.

Gene expression databases

Bgeei A5D6R3.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR028406. PLC-delta3.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
PTHR10336:SF33. PTHR10336:SF33. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00169. PH. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00233. PH. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  2. "Duplication of phospholipase C-delta gene family in fish genomes."
    Kim M.S., Seo J.S., Ahn S.J., Kim N.Y., Je J.E., Sung J.H., Lee H.H., Chung J.K.
    Genomics 92:366-371(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE DUPLICATION.

Entry informationi

Entry nameiPLD3A_DANRE
AccessioniPrimary (citable) accession number: A5D6R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: June 12, 2007
Last modified: September 3, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi