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A5D6R3 (PLD3A_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3-A

EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase C-delta-3-A
Phospholipase C-delta-3-A
Short name=PLC-delta-3-A
Gene names
Name:plcd3a
ORF Names:zgc:158396
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length784 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca2+ from intracellular stores By similarity.

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactor

Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain By similarity.

Subcellular location

Membrane; Peripheral membrane protein By similarity. Cytoplasm By similarity.

Domain

The C2 domain is a Ca2+-dependent membrane-targeting module By similarity.

The PH domain mediates interaction with the surface membrane by binding to PIP2 By similarity.

Sequence similarities

Contains 1 C2 domain.

Contains 3 EF-hand domains.

Contains 1 PH domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7847841-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3-A
PRO_0000306823

Regions

Domain38 – 149112PH
Domain159 – 19436EF-hand 1
Domain195 – 23036EF-hand 2
Domain227 – 26236EF-hand 3
Domain313 – 458146PI-PLC X-box
Domain506 – 621116PI-PLC Y-box
Domain628 – 733106C2
Calcium binding172 – 183121 Potential
Calcium binding208 – 219122 Potential
Region48 – 7831Substrate binding By similarity

Sites

Active site3281 By similarity
Active site3731 By similarity
Metal binding3291Calcium 1; catalytic By similarity
Metal binding3581Calcium 1; catalytic By similarity
Metal binding3601Calcium 1; catalytic By similarity
Metal binding4071Calcium 1; catalytic By similarity
Metal binding6641Calcium 2; via carbonyl oxygen By similarity
Metal binding6661Calcium 2 By similarity
Metal binding6901Calcium 2 By similarity
Metal binding7191Calcium 3 By similarity
Metal binding7211Calcium 3 By similarity
Binding site4561Substrate By similarity
Binding site4581Substrate By similarity
Binding site5341Substrate By similarity
Binding site5611Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A5D6R3 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 4078823F379343C6

FASTA78489,381
        10         20         30         40         50         60 
MLGRKKNPET VQTESKSVES KTHDPLRRLG VLDDEDVLLM LQGSKMMKVR SQRWRKDRRL 

        70         80         90        100        110        120 
KLLEDCVTVW CESSKTSRKS NRQQTFSVTE VECVREGCQS ECLRRMTDLV PEKNCFTVVF 

       130        140        150        160        170        180 
RGGRKSLDLC CHTQEEAERW VRGIRTLKDR VSNMSQKEKL DHWIRGYLRR ADQNQDGKMS 

       190        200        210        220        230        240 
YDEVKHLLQL INIDLNEQYA RTLFKKCDRS CDGRLDHVEI EEFCREMMRR PELDAVFRHY 

       250        260        270        280        290        300 
SGNGCVLTTL ELRDFLGDQG EDASLVHAKS LIQTFELNDW AQKNLFMTQN GFTMYMLSKE 

       310        320        330        340        350        360 
NDVFNPDHTH VYQDMSKPLA HYYISSSHNT YLTKDQVTSA SSTEPYIRAL NQGCRCVELD 

       370        380        390        400        410        420 
CWDGDKGEPI IYHGHTLTSK VLFKEVIETI AQYAFKASPY PLILSLENHC SVEQQAIMAQ 

       430        440        450        460        470        480 
QLQSILGKKL LAKPLSDLPL KQLPSPEELK GRILLKGKKL NGLLGKTESW TSFTNSSDEE 

       490        500        510        520        530        540 
SVAGGNKKES KKDLARSAST KLSPELSDLV VYCQSVPFSG FETANQRPPS VITSFSENEA 

       550        560        570        580        590        600 
LKLIKDSGKL FVRNNSRQLS RIYPSAQRLQ SSNFDPQDMW NAGCQMVALN FQTPGEQMDL 

       610        620        630        640        650        660 
NQGRFLPNGR CGYVLKPEFL CDPKSDFDPE NTGGGPGHIP TQLTIRVISA QQLPKINTDK 

       670        680        690        700        710        720 
PNSIVDPQVW VEIHGVSIDK ARAKTQRIDN NGFNPRWDCT VSFQLQLPEL ALVRFMVEDH 

       730        740        750        760        770        780 
DHKSKNDFIG QFTLPFTSLR TGYRHVHLLK ADGSTLSPAT LFIHVKVVRR GVHIKTVSER 


IGKA 

« Hide

References

« Hide 'large scale' references
[1]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[2]"Duplication of phospholipase C-delta gene family in fish genomes."
Kim M.S., Seo J.S., Ahn S.J., Kim N.Y., Je J.E., Sung J.H., Lee H.H., Chung J.K.
Genomics 92:366-371(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE DUPLICATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC139849 mRNA. Translation: AAI39850.1.
RefSeqNP_001092893.1. NM_001099423.1.
UniGeneDr.50929.

3D structure databases

ProteinModelPortalA5D6R3.
SMRA5D6R3. Positions 219-768.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000093449.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000102674; ENSDARP00000093449; ENSDARG00000052957.
GeneID569040.
KEGGdre:569040.

Organism-specific databases

CTD569040.
ZFINZDB-GENE-070620-1. plcd3a.

Phylogenomic databases

eggNOGNOG149692.
GeneTreeENSGT00740000114979.
HOGENOMHOG000006871.
KOK05857.
OMADPQDMWN.
OrthoDBEOG7V49XT.
PhylomeDBA5D6R3.
TreeFamTF313216.

Gene expression databases

BgeeA5D6R3.

Family and domain databases

Gene3D1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR028406. PLC-delta3.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR001849. Pleckstrin_homology.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF33. PTHR10336:SF33. 1 hit.
PfamPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00169. PH. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00233. PH. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20889471.
PROA5D6R3.

Entry information

Entry namePLD3A_DANRE
AccessionPrimary (citable) accession number: A5D6R3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: June 12, 2007
Last modified: April 16, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families