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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi105ZincUniRule annotation1
Metal bindingi107ZincUniRule annotation1
Metal bindingi132ZincUniRule annotation1
Metal bindingi134ZincUniRule annotation1
Binding sitei252ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:PTH_0293
OrganismiPelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI)
Taxonomic identifieri370438 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaePelotomaculum
Proteomesi
  • UP000006556 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003677321 – 480Glutamate--tRNA ligaseAdd BLAST480

Proteomic databases

PRIDEiA5D5L8

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi370438.PTH_0293

Structurei

3D structure databases

ProteinModelPortaliA5D5L8
SMRiA5D5L8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi8 – 18"HIGH" regionUniRule annotationAdd BLAST11
Motifi249 – 253"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK09698
OMAiAYYAFDT
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A5D5L8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRVRFAPSP TGPLHIGGAR SALFNWLFAR RYGGKFIVRI EDTDLERSSR
60 70 80 90 100
QSEENILAAL RWLGLDWDEG VDAGGPCEPY RQTERLHLYR HYAEILLQSG
110 120 130 140 150
AAYRCYCTEE ELAAEREALM EKGETPRYLG RCRDLTPQER ARLEAEGRKA
160 170 180 190 200
AVRFRVPRDR VIQVKDLVRG DVSFECNGMG DFIIMKSDGI PTYNFAVVVD
210 220 230 240 250
DHTMAISHVI RAEEHLPNTP RQILLYQALG WDTPEFAHVS LILGKDRSKM
260 270 280 290 300
SKRHGATAVE QYREKGYLPE AVVNFLALLG WSPGGENEVL SIEELKQQFS
310 320 330 340 350
LEKVSKSPAV FDLDKLNWLN GYYIRQSSLE RVAELAVPFL EKAGYIRAPL
360 370 380 390 400
SPEEFERVKM MVASVRKYLN CMEEVIDHVK IYFTDNFNNL DEDAREVMAG
410 420 430 440 450
QQVPAVLAAL KDKVASGGKL TGAAARAALK EVGKELGVKG QQIFMPVRVA
460 470 480
VSGNTHGPDL DQIMAILGKD EVVKRLSRWV
Length:480
Mass (Da):54,098
Last modified:June 12, 2007 - v1
Checksum:i89F0E27DD52F19CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009389 Genomic DNA Translation: BAF58474.1

Genome annotation databases

EnsemblBacteriaiBAF58474; BAF58474; PTH_0293
KEGGipth:PTH_0293

Similar proteinsi

Entry informationi

Entry nameiSYE_PELTS
AccessioniPrimary (citable) accession number: A5D5L8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 12, 2007
Last modified: May 23, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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