Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei586 – 5861ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPTHE370438:GCGQ-865-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:PTH_0839
OrganismiPelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI)
Taxonomic identifieri370438 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaePelotomaculum
ProteomesiUP000006556: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 827827Leucine--tRNA ligasePRO_0000334789Add
BLAST

Proteomic databases

PRIDEiA5D416.

Interactioni

Protein-protein interaction databases

STRINGi370438.PTH_0839.

Structurei

3D structure databases

ProteinModelPortaliA5D416.
SMRiA5D416. Positions 3-762.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi583 – 5875"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5D416-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKERYDFKEI EEKWQARWAA QDLYAVPDYS DRPKYYCLEM FPYPSGKLHM
60 70 80 90 100
GHVRNYSIGD VVARFKTMQG YHVLHPMGWD AFGLPAENAA IKHGGVHPAE
110 120 130 140 150
WTLDNIESMR AQLKQLGISY DWNREVATCH PGYYRWTQWL FLQLFHNGLA
160 170 180 190 200
YKKKAAVNWC PGCATVLANE QVKDGGCERC KAPVEKRELE QWFFKITDYA
210 220 230 240 250
ERLLKDLELL EGWPEKVKIM QENWIGRSEG AEIDFKVEGS DDIITVYTTR
260 270 280 290 300
PDTVFGVTYM VLAPEHPLVE KLIAGSKQEA EIKEFIRKVR NLREIDRTST
310 320 330 340 350
EAEKVGMPTG AHCINPLTGE KVPVLIANYV LMEYGTGCVM GVPAHDQRDF
360 370 380 390 400
EFARKYGYPI RVVIQPPGVE LDPAAMEAAY EEEGFLVNSG PFSGMPNKEA
410 420 430 440 450
IRAITRHLEE KGRGRFRVTY RLRDWLISRQ RYWGAPIPII YCDRCGTVPV
460 470 480 490 500
PESDLPVLLP MDVEFKPTGQ SPLAECPEFV NAACPSCGGP GKRETDTMDT
510 520 530 540 550
FMCSSWYYYR YTSPRDNDAP WDRNKVDYWL PVDQYIGGVE HAILHLLYSR
560 570 580 590 600
FFTKVLYDLK LVSNLEPFSN LLTQGMVLKD GAKMSKSRGN VVSPEDIVAR
610 620 630 640 650
YGADTARLFI LFAAPPERDL EWSDQGVEGC YRFLNRVWRL VMPLAGLLKE
660 670 680 690 700
APAAVSGKLV GANREMRRVT HSTIKKVTED ISARFNFNTA VSAIMELVNA
710 720 730 740 750
LYQFREIPES DRNPAVLREA VESLLLLLAP FAPHITEELW EATGHRGSIH
760 770 780 790 800
LQPWPSYDPE AIAEDEITIV VQINGRVRER LLVPAGITPQ EMQDRVMKEP
810 820
RVMRMVEGKK VAKVITVPGK LVNIVIK
Length:827
Mass (Da):94,245
Last modified:June 12, 2007 - v1
Checksum:i963D6FAEEB49ADC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009389 Genomic DNA. Translation: BAF59020.1.
RefSeqiYP_001211389.1. NC_009454.1.

Genome annotation databases

EnsemblBacteriaiBAF59020; BAF59020; PTH_0839.
GeneIDi5140461.
KEGGipth:PTH_0839.
PATRICi22908665. VBIPelThe8413_0946.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009389 Genomic DNA. Translation: BAF59020.1.
RefSeqiYP_001211389.1. NC_009454.1.

3D structure databases

ProteinModelPortaliA5D416.
SMRiA5D416. Positions 3-762.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi370438.PTH_0839.

Proteomic databases

PRIDEiA5D416.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF59020; BAF59020; PTH_0839.
GeneIDi5140461.
KEGGipth:PTH_0839.
PATRICi22908665. VBIPelThe8413_0946.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciPTHE370438:GCGQ-865-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Pelotomaculum thermopropionicum reveals niche-associated evolution in anaerobic microbiota."
    Kosaka T., Kato S., Shimoyama T., Ishii S., Abe T., Watanabe K.
    Genome Res. 18:442-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13744 / JCM 10971 / SI.

Entry informationi

Entry nameiSYL_PELTS
AccessioniPrimary (citable) accession number: A5D416
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: February 4, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.