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A5D416 (SYL_PELTS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PTH_0839
OrganismPelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI) [Complete proteome] [HAMAP]
Taxonomic identifier370438 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptococcaceaePelotomaculum

Protein attributes

Sequence length827 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 827827Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334789

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif583 – 5875"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5861ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5D416 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 963D6FAEEB49ADC3

FASTA82794,245
        10         20         30         40         50         60 
MKERYDFKEI EEKWQARWAA QDLYAVPDYS DRPKYYCLEM FPYPSGKLHM GHVRNYSIGD 

        70         80         90        100        110        120 
VVARFKTMQG YHVLHPMGWD AFGLPAENAA IKHGGVHPAE WTLDNIESMR AQLKQLGISY 

       130        140        150        160        170        180 
DWNREVATCH PGYYRWTQWL FLQLFHNGLA YKKKAAVNWC PGCATVLANE QVKDGGCERC 

       190        200        210        220        230        240 
KAPVEKRELE QWFFKITDYA ERLLKDLELL EGWPEKVKIM QENWIGRSEG AEIDFKVEGS 

       250        260        270        280        290        300 
DDIITVYTTR PDTVFGVTYM VLAPEHPLVE KLIAGSKQEA EIKEFIRKVR NLREIDRTST 

       310        320        330        340        350        360 
EAEKVGMPTG AHCINPLTGE KVPVLIANYV LMEYGTGCVM GVPAHDQRDF EFARKYGYPI 

       370        380        390        400        410        420 
RVVIQPPGVE LDPAAMEAAY EEEGFLVNSG PFSGMPNKEA IRAITRHLEE KGRGRFRVTY 

       430        440        450        460        470        480 
RLRDWLISRQ RYWGAPIPII YCDRCGTVPV PESDLPVLLP MDVEFKPTGQ SPLAECPEFV 

       490        500        510        520        530        540 
NAACPSCGGP GKRETDTMDT FMCSSWYYYR YTSPRDNDAP WDRNKVDYWL PVDQYIGGVE 

       550        560        570        580        590        600 
HAILHLLYSR FFTKVLYDLK LVSNLEPFSN LLTQGMVLKD GAKMSKSRGN VVSPEDIVAR 

       610        620        630        640        650        660 
YGADTARLFI LFAAPPERDL EWSDQGVEGC YRFLNRVWRL VMPLAGLLKE APAAVSGKLV 

       670        680        690        700        710        720 
GANREMRRVT HSTIKKVTED ISARFNFNTA VSAIMELVNA LYQFREIPES DRNPAVLREA 

       730        740        750        760        770        780 
VESLLLLLAP FAPHITEELW EATGHRGSIH LQPWPSYDPE AIAEDEITIV VQINGRVRER 

       790        800        810        820 
LLVPAGITPQ EMQDRVMKEP RVMRMVEGKK VAKVITVPGK LVNIVIK 

« Hide

References

[1]"The genome of Pelotomaculum thermopropionicum reveals niche-associated evolution in anaerobic microbiota."
Kosaka T., Kato S., Shimoyama T., Ishii S., Abe T., Watanabe K.
Genome Res. 18:442-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13744 / JCM 10971 / SI.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009389 Genomic DNA. Translation: BAF59020.1.
RefSeqYP_001211389.1. NC_009454.1.

3D structure databases

ProteinModelPortalA5D416.
SMRA5D416. Positions 3-762.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING370438.PTH_0839.

Proteomic databases

PRIDEA5D416.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF59020; BAF59020; PTH_0839.
GeneID5140461.
KEGGpth:PTH_0839.
PATRIC22908665. VBIPelThe8413_0946.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPTHE370438:GCGQ-865-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PELTS
AccessionPrimary (citable) accession number: A5D416
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries