Skip Header

Contribute Send feedback
Read comments (?) or add your own

A5D3G6 (TGT_PELTS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase
EC=2.4.2.29
Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase
Gene names
Name:tgt
Ordered Locus Names:PTH_1032
OrganismPelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI) [Complete proteome] [HAMAP]
Taxonomic identifier370438 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Pelotomaculum ›

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_00168

Catalytic activity

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_00168

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168

Cofactor

Binds 1 zinc ion per subunit By similarity.

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
tRNA processing
   LigandMetal-binding
Zinc
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processqueuosine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

queuine tRNA-ribosyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Queuine tRNA-ribosyltransferase HAMAP-Rule MF_00168
PRO_1000077013

Sites

Active site931Nucleophile By similarity
Metal binding3041Zinc By similarity
Metal binding3061Zinc By similarity
Metal binding3091Zinc By similarity
Metal binding3351Zinc By similarity
Binding site941Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A5D3G6 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: C13776522FEDE239

FASTA37041,883
        10         20         30         40         50         60 
MAVSFTITYK DESTGARLGL LYTPHGTVET PVFMPVGTQA TVKTMTPEEV RDIGGRMILS 

        70         80         90        100        110        120 
NTYHLYLRPG HELIREAGGL HRFMHWDGPI LTDSGGFQVF SLGPLRKVSE EGVAFRSHID 

       130        140        150        160        170        180 
GSEHFFSPEK AMEVQMALGS DIAMAFDECA PYPCSREYAL AATERTTRWA RRCRTAHDRE 

       190        200        210        220        230        240 
DQGLFGIVQG GTFKDLRERS ARELVELDFP GYAIGGLSVG EPKQLMYEVL DYTVPLLPEE 

       250        260        270        280        290        300 
KPRYLMGVGS PDCLVEGVLR GIDMFDCVLP TRIARNGTAL TRQGRLVVRN AEYARDFSPL 

       310        320        330        340        350        360 
EPDCDCYACR NYTRAYIRHL IKANEILGIR LTTIHNLYFI MKLMEEIRAA VRQGRMLQFR 

       370 
DDFLRKYQGD

« Hide

References

[1]"The genome of Pelotomaculum thermopropionicum reveals niche-associated evolution in anaerobic microbiota."
Kosaka T., Kato S., Shimoyama T., Ishii S., Abe T., Watanabe K.
Genome Res. 18:442-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13744 / JCM 10971 / SI.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP009389 Genomic DNA. Translation: BAF59213.1.
RefSeqYP_001211582.1. NC_009454.1.

3D structure databases

ProteinModelPortalA5D3G6.
SMRA5D3G6. Positions 3-367.
ModBaseSearch...

Protein-protein interaction databases

STRING370438.PTH_1032.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF59213; BAF59213; PTH_1032.
GeneID5138389.
KEGGpth:PTH_1032.
PATRIC22909123. VBIPelThe8413_1159.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
HOGENOMHOG000223473.
KOK00773.
OMAMSPERSI.
ProtClustDBPRK00112.

Enzyme and pathway databases

UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTGT_PELTS
AccessionPrimary (citable) accession number: A5D3G6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents