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A5CY09 (SYL_VESOH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:COSY_0005
OrganismVesicomyosocius okutanii subsp. Calyptogena okutanii (strain HA) [Complete proteome] [HAMAP]
Taxonomic identifier412965 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriasulfur-oxidizing symbionts

Protein attributes

Sequence length815 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 815815Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009461

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif571 – 5755"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5741ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5CY09 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 98F196937EC5C85E

FASTA81595,149
        10         20         30         40         50         60 
MNIKYDVQKI EKQAQKYWKE KKSFEVIEDY SKEKYYCLSM FPYPSGRLHM GHVRNYSIGD 

        70         80         90        100        110        120 
VISRFQRMQG KNVMQPIGWD GFGLPAENAA LKNKESPAKW TYKNINYMKT QLNQLGFGYD 

       130        140        150        160        170        180 
WAREITTCHP KYYRWEQWLF IKLFKKNLIY KKKAIVNWDP VDQTVLANEQ VINGRGWRSN 

       190        200        210        220        230        240 
ALIEKKEISQ WFIRITNYAE ELLNDLDKLY GWPDPVKIMQ KNWIGKSIGL EITFSRRNSD 

       250        260        270        280        290        300 
PLIIYTTRPD TLMGVTYLAI SFEHPLALES GKNNYQVQSF IEKCKTIQTS EILNEIMDKK 

       310        320        330        340        350        360 
GIDSGFKCIH PITNNEVPIW ITNFVLMSYG TGAIMSVPAH DKRDFKFAKK YGIFIKQVIN 

       370        380        390        400        410        420 
KNESIDKGPI INKGKLFNSE EFSGMDFNQA YESIAKTLIE KNLGNKKINY RLRDWGISRQ 

       430        440        450        460        470        480 
RYWGCPIPIV NCKYCGSVTV KVKDLPVILP EKVKFYDVSS PIKKMPNFYQ TICPKCGSKA 

       490        500        510        520        530        540 
HRETDTFDTF FESSWYFARH TCNNNNNAML DKRVNYWLEV DQYIGGVEHS ILHLLYARFF 

       550        560        570        580        590        600 
NKLLRDEGLI KYDEPFKNLL TQGMVLKNGV KMSKSKGNTV DPTKMIKKYG ADTVRLFILF 

       610        620        630        640        650        660 
AAPPMQDLEW NNSGLEGAHR FIKKVYRLVS IYINDSKDYI VNHLNINFLN KTQKHIRRKI 

       670        680        690        700        710        720 
HQNLVKITDD INRRYTFNTA ISTLMESVDI INKFTKTDTQ SIALRSESIN IILLTLSPIT 

       730        740        750        760        770        780 
PHICHYLWLK LGNKKAIINE PWPKVDLKAL IESEVQIIIQ VDGKLRDKMM MMINTDKEIL 

       790        800        810 
ESEVLSNKNI IKFTKNKNII KIIIIHNKLI NIVTK 

« Hide

References

[1]"Reduced genome of the thioautotrophic intracellular symbiont in a deep-sea clam, Calyptogena okutanii."
Kuwahara H., Yoshida T., Takaki Y., Shimamura S., Nishi S., Harada M., Matsuyama K., Takishita K., Kawato M., Uematsu K., Fujiwara Y., Sato T., Kato C., Kitagawa M., Kato I., Maruyama T.
Curr. Biol. 17:881-886(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HA.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009247 Genomic DNA. Translation: BAF61144.1.
RefSeqYP_001218868.1. NC_009465.1.

3D structure databases

ProteinModelPortalA5CY09.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING412965.COSY_0005.

Proteomic databases

PRIDEA5CY09.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF61144; BAF61144; COSY_0005.
GeneID5171803.
KEGGvok:COSY_0005.
PATRIC32019815. VBICanVes128383_0005.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCVES412965:GHZZ-5-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_VESOH
AccessionPrimary (citable) accession number: A5CY09
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries