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A5CX61

- SYI_VESOH

UniProt

A5CX61 - SYI_VESOH

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Vesicomyosocius okutanii subsp. Calyptogena okutanii (strain HA)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
    Binding sitei596 – 5961ATPUniRule annotation
    Metal bindingi918 – 9181ZincUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation
    Metal bindingi938 – 9381ZincUniRule annotation
    Metal bindingi941 – 9411ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCVES412965:GHZZ-321-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:COSY_0322
    OrganismiVesicomyosocius okutanii subsp. Calyptogena okutanii (strain HA)
    Taxonomic identifieri412965 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriasulfur-oxidizing symbionts
    ProteomesiUP000000247: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 955955Isoleucine--tRNA ligasePRO_1000022140Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi412965.COSY_0322.

    Structurei

    3D structure databases

    ProteinModelPortaliA5CX61.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi593 – 5975"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKKRIELM.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A5CX61-1 [UniParc]FASTAAdd to Basket

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    MSDYKSSLNL PSTQFSMKAN LANREGKFLK KWQNDRLYDQ IRKQNQGKPK    50
    FVLHDGPIYA NGDIHIGHAV NKVLKDIIVK SKSLSGFDAP YVPGWDCHGL 100
    PIELNVEKEY GKVGIKIDVN TFRYKCREYA DHQVMRQSQD FQRLGILSDW 150
    DNPYLTKDFK YEADVVRALG QVVKNGHVYK GHKPLHWCTE CGSALAEAEV 200
    EYKNKQSEAI DVKFRIIEDS VFNVKKPVSV VIWTTTPWTL PANEAVALHS 250
    ELNYVLVDIG SEYLLLSQSL VVNSISRYDI KVTIGERMFS SSELEGLKVQ 300
    HPFYDKQVPI ILGDHVTIDS GTGAVHIAPA HGQEDFIAGL KYNLPIDCPV 350
    DAKGVFFKEI LLLGGQFIFK ANASVIRILK ETNTLVKHES LTHSYPHCWR 400
    HKTPIIFRIT SQWFISMQQN GLRDIVNSEI QKVQWIPHWS KKRIELMVDN 450
    RPDWCISRQR FWGVPITLFV HKKTGELHPN TQMLFVCIAN RIEQEGIEAW 500
    FKSDTKDFIG DDVNDYDKIT DILDVWFDSG MSHFVVLKVR KELSNVADLY 550
    LEGSDQHRGW FQSSLISSVA INKKAPYKNV LTHGFVVDKD GKKMSKSLGN 600
    IISPQKIVNN VGADILRLWI ASTDYTGEMT VSDEILKRSA DSYRRIRNTM 650
    RFMLANMNGF TQKNLVDTKA MLDLDRWIVA KTQKIQEAII ENYDTYQFHY 700
    IVKSINNFCS NDLGGFYLDI IKDRQYTTQK DSPARRSAQT ALYHITQMMV 750
    RWLSPILSFT SEEIWQELAP NKKSIFLQEW YLQVDTIDNV VFDDGIIYGT 800
    MISSHISQEQ REGLEKSDDI FTSINIVRII SPTIRQAIEK LRKDKVLGAS 850
    LEAEVDIYCN LKVKEKLSKF GEELRFMFIT SDVRLHSFEE KPNNAIEVDS 900
    DVLQQVAIVV VKSEHSKCVR CWHHRKDVGS NNKYLELCCR CVENVDGDGE 950
    VRKFA 955
    Length:955
    Mass (Da):109,700
    Last modified:June 12, 2007 - v1
    Checksum:i5D73688DC5DC10A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009247 Genomic DNA. Translation: BAF61447.1.
    RefSeqiYP_001219171.1. NC_009465.1.

    Genome annotation databases

    EnsemblBacteriaiBAF61447; BAF61447; COSY_0322.
    GeneIDi5172097.
    KEGGivok:COSY_0322.
    PATRICi32020463. VBICanVes128383_0317.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009247 Genomic DNA. Translation: BAF61447.1 .
    RefSeqi YP_001219171.1. NC_009465.1.

    3D structure databases

    ProteinModelPortali A5CX61.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 412965.COSY_0322.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAF61447 ; BAF61447 ; COSY_0322 .
    GeneIDi 5172097.
    KEGGi vok:COSY_0322.
    PATRICi 32020463. VBICanVes128383_0317.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KKRIELM.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CVES412965:GHZZ-321-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Reduced genome of the thioautotrophic intracellular symbiont in a deep-sea clam, Calyptogena okutanii."
      Kuwahara H., Yoshida T., Takaki Y., Shimamura S., Nishi S., Harada M., Matsuyama K., Takishita K., Kawato M., Uematsu K., Fujiwara Y., Sato T., Kato C., Kitagawa M., Kato I., Maruyama T.
      Curr. Biol. 17:881-886(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HA.

    Entry informationi

    Entry nameiSYI_VESOH
    AccessioniPrimary (citable) accession number: A5CX61
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3