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A5CWW0

- BIOB_VESOH

UniProt

A5CWW0 - BIOB_VESOH

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Protein
Biotin synthase
Gene
bioB, COSY_0459
Organism
Vesicomyosocius okutanii subsp. Calyptogena okutanii (strain HA)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi58 – 581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi98 – 981Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi129 – 1291Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi189 – 1891Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi261 – 2611Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCVES412965:GHZZ-458-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:COSY_0459
OrganismiVesicomyosocius okutanii subsp. Calyptogena okutanii (strain HA)
Taxonomic identifieri412965 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriasulfur-oxidizing symbionts
ProteomesiUP000000247: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Biotin synthaseUniRule annotation
PRO_0000381694Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi412965.COSY_0459.

Structurei

3D structure databases

ProteinModelPortaliA5CWW0.
SMRiA5CWW0. Positions 5-315.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A5CWW0-1 [UniParc]FASTAAdd to Basket

« Hide

MTQQRHNWTL KEVEALFTLP FNDLLFQAHS IHRKNFDPNQ VQISSLLNIK    50
TGACPEDCLY CSQSSKYDTG LEREKLMEID LVLKQAKQAQ DNGATRFCMG 100
AAWRNPTDKS INKVIPMIQG VKDMGMETCV TLGMLTQKQA FVLKEVGLDY 150
YNHNIDTSKE NYSNIVTTRN FQDRLNTLES VQNANIRVCS GGILGLGEDQ 200
TDRASMLRSL SNLAIHPDSV PLNLLVPIPG TPFENIEPPT ESEFIRTIAV 250
ARIMMPKSVV RLSAGRTKMG DTMQALCFFA GANSIFYGEQ LLTTDNPNTD 300
SDQDLFVRLG INQKQANNLQ SV 322
Length:322
Mass (Da):36,014
Last modified:June 12, 2007 - v1
Checksum:iFC23F04EEB95BCF6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009247 Genomic DNA. Translation: BAF61578.1.
RefSeqiWP_011929848.1. NC_009465.1.
YP_001219302.1. NC_009465.1.

Genome annotation databases

EnsemblBacteriaiBAF61578; BAF61578; COSY_0459.
GeneIDi5172706.
KEGGivok:COSY_0459.
PATRICi32020757. VBICanVes128383_0458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009247 Genomic DNA. Translation: BAF61578.1 .
RefSeqi WP_011929848.1. NC_009465.1.
YP_001219302.1. NC_009465.1.

3D structure databases

ProteinModelPortali A5CWW0.
SMRi A5CWW0. Positions 5-315.
ModBasei Search...

Protein-protein interaction databases

STRINGi 412965.COSY_0459.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAF61578 ; BAF61578 ; COSY_0459 .
GeneIDi 5172706.
KEGGi vok:COSY_0459.
PATRICi 32020757. VBICanVes128383_0458.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci CVES412965:GHZZ-458-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Reduced genome of the thioautotrophic intracellular symbiont in a deep-sea clam, Calyptogena okutanii."
    Kuwahara H., Yoshida T., Takaki Y., Shimamura S., Nishi S., Harada M., Matsuyama K., Takishita K., Kawato M., Uematsu K., Fujiwara Y., Sato T., Kato C., Kitagawa M., Kato I., Maruyama T.
    Curr. Biol. 17:881-886(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HA.

Entry informationi

Entry nameiBIOB_VESOH
AccessioniPrimary (citable) accession number: A5CWW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 12, 2007
Last modified: September 3, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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