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A5CUV9 (MEND_CLAM3) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Short name=SEPHCHC synthase
EC=2.2.1.9
Alternative name(s):
Menaquinone biosynthesis protein MenD
Gene names
Name:menD
Ordered Locus Names:CMM_2812
OrganismClavibacter michiganensis subsp. michiganensis (strain NCPPB 382) [Complete proteome] [HAMAP]
Taxonomic identifier443906 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter

Protein attributes

Sequence length588 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity. HAMAP-Rule MF_01659

Catalytic activity

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2. HAMAP-Rule MF_01659

Cofactor

Magnesium or manganese By similarity. HAMAP-Rule MF_01659

Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathway

Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 2/8. HAMAP-Rule MF_01659

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01659

Sequence similarities

Belongs to the TPP enzyme family. MenD subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5885882-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase HAMAP-Rule MF_01659
PRO_0000341724

Sequences

Sequence LengthMass (Da)Tools
A5CUV9 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 52D8F8F15A9CB078

FASTA58861,363
        10         20         30         40         50         60 
MTTTGSLPAQ PSSTSPRTGN PSTDRALAML LALVREGVTD VVLCPGSRSQ ALALVAAELE 

        70         80         90        100        110        120 
RVDGVRLHVR IDERAAGFLA LGLGVESGRP APVITTSGTA VANLHPAVLE GWHSGVPMLL 

       130        140        150        160        170        180 
LTGDRPAELR GIASNQTTRQ PGMFGDRVVV VDVPAPEETD EDLSRDARLA RDAYRRARDE 

       190        200        210        220        230        240 
RTPVHVNVAF RDPLSVAVPD LAEAVAEAHA AADAEAAAAP APAGPTAADV LDLPHGPRTL 

       250        260        270        280        290        300 
VVAGHAAGEA AEELARAGGW PLAAEISSGS HFGPNLVVSF RELLARPGFG DRVERVIVFG 

       310        320        330        340        350        360 
HPTLTREVPL LVGREDVEAI VVGSTGGEDY DPRHRVTAHP AAVRVVGEPA DPAEARRWTG 

       370        380        390        400        410        420 
TWVQASRAIL DEASAAESAP LLPSGTTPAE RRDFARAELA AVRADVTRRH LVRALWQATW 

       430        440        450        460        470        480 
PHDRLVLGAS RLIREADRAL PGKRVRVHAN RGLAGIDGTI STGLGIALAS QAGSGSAAAG 

       490        500        510        520        530        540 
ITRVLVGDLT LLHDVGSLLI GTGERVPRIQ VIVGNDGGGT IFDGLEVANT AAPAAIDRVM 

       550        560        570        580 
FTPQRVDLAS LAKAYGWTHL RAATHGELEA ALTTASGAPL LIEVPLVR 

« Hide

References

[1]"The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity."
Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C. expand/collapse author list , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D.
J. Bacteriol. 190:2138-2149(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCPPB 382.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM711867 Genomic DNA. Translation: CAN02897.1.
RefSeqYP_001223557.1. NC_009480.1.

3D structure databases

ProteinModelPortalA5CUV9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING443906.CMM_2812.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAN02897; CAN02897; CMM_2812.
GeneID5173302.
KEGGcmi:CMM_2812.
PATRIC21456487. VBIClaMic82482_2937.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1165.
HOGENOMHOG000218359.
KOK02551.
OMAWRSAVCR.
OrthoDBEOG6NWBQW.

Enzyme and pathway databases

BioCycCMIC443906:GCI2-2898-MONOMER.
UniPathwayUPA00079; UER00164.

Family and domain databases

Gene3D3.40.50.970. 2 hits.
HAMAPMF_01659. MenD.
InterProIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamPF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFPIRSF004983. MenD. 1 hit.
SUPFAMSSF52518. SSF52518. 2 hits.
TIGRFAMsTIGR00173. menD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMEND_CLAM3
AccessionPrimary (citable) accession number: A5CUV9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: June 12, 2007
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways