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A5CUV9

- MEND_CLAM3

UniProt

A5CUV9 - MEND_CLAM3

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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene
menD, CMM_2812
Organism
Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity.UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Magnesium or manganese By similarity.UniRule annotation
Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Menaquinone biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciCMIC443906:GCI2-2898-MONOMER.
UniPathwayiUPA00079; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (EC:2.2.1.9)
Short name:
SEPHCHC synthase
Alternative name(s):
Menaquinone biosynthesis protein MenD
Gene namesi
Name:menD
Ordered Locus Names:CMM_2812
OrganismiClavibacter michiganensis subsp. michiganensis (strain NCPPB 382)
Taxonomic identifieri443906 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter
ProteomesiUP000001564: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5885882-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotationPRO_0000341724Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi443906.CMM_2812.

Structurei

3D structure databases

ProteinModelPortaliA5CUV9.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1165.
HOGENOMiHOG000218359.
KOiK02551.
OMAiWRSAVCR.
OrthoDBiEOG6NWBQW.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

A5CUV9-1 [UniParc]FASTAAdd to Basket

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MTTTGSLPAQ PSSTSPRTGN PSTDRALAML LALVREGVTD VVLCPGSRSQ    50
ALALVAAELE RVDGVRLHVR IDERAAGFLA LGLGVESGRP APVITTSGTA 100
VANLHPAVLE GWHSGVPMLL LTGDRPAELR GIASNQTTRQ PGMFGDRVVV 150
VDVPAPEETD EDLSRDARLA RDAYRRARDE RTPVHVNVAF RDPLSVAVPD 200
LAEAVAEAHA AADAEAAAAP APAGPTAADV LDLPHGPRTL VVAGHAAGEA 250
AEELARAGGW PLAAEISSGS HFGPNLVVSF RELLARPGFG DRVERVIVFG 300
HPTLTREVPL LVGREDVEAI VVGSTGGEDY DPRHRVTAHP AAVRVVGEPA 350
DPAEARRWTG TWVQASRAIL DEASAAESAP LLPSGTTPAE RRDFARAELA 400
AVRADVTRRH LVRALWQATW PHDRLVLGAS RLIREADRAL PGKRVRVHAN 450
RGLAGIDGTI STGLGIALAS QAGSGSAAAG ITRVLVGDLT LLHDVGSLLI 500
GTGERVPRIQ VIVGNDGGGT IFDGLEVANT AAPAAIDRVM FTPQRVDLAS 550
LAKAYGWTHL RAATHGELEA ALTTASGAPL LIEVPLVR 588
Length:588
Mass (Da):61,363
Last modified:June 12, 2007 - v1
Checksum:i52D8F8F15A9CB078
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM711867 Genomic DNA. Translation: CAN02897.1.
RefSeqiYP_001223557.1. NC_009480.1.

Genome annotation databases

EnsemblBacteriaiCAN02897; CAN02897; CMM_2812.
GeneIDi5173302.
KEGGicmi:CMM_2812.
PATRICi21456487. VBIClaMic82482_2937.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM711867 Genomic DNA. Translation: CAN02897.1 .
RefSeqi YP_001223557.1. NC_009480.1.

3D structure databases

ProteinModelPortali A5CUV9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 443906.CMM_2812.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAN02897 ; CAN02897 ; CMM_2812 .
GeneIDi 5173302.
KEGGi cmi:CMM_2812.
PATRICi 21456487. VBIClaMic82482_2937.

Phylogenomic databases

eggNOGi COG1165.
HOGENOMi HOG000218359.
KOi K02551.
OMAi WRSAVCR.
OrthoDBi EOG6NWBQW.

Enzyme and pathway databases

UniPathwayi UPA00079 ; UER00164 .
BioCyci CMIC443906:GCI2-2898-MONOMER.

Family and domain databases

Gene3Di 3.40.50.970. 2 hits.
HAMAPi MF_01659. MenD.
InterProi IPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view ]
Pfami PF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004983. MenD. 1 hit.
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00173. menD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity."
    Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C.
    , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D.
    J. Bacteriol. 190:2138-2149(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCPPB 382.

Entry informationi

Entry nameiMEND_CLAM3
AccessioniPrimary (citable) accession number: A5CUV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: June 12, 2007
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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