Reviewed, UniProtKB/Swiss-Prot A5CTY4 (ISPDF_CLAM3)

Names and origin

Protein names

Recommended name:
    Bifunctional enzyme ispD/ispF
Including the following 2 domains:
    1- Recommended name:
            2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
              EC=2.7.7.60
        Alternative name(s):
            4-diphosphocytidyl-2C-methyl-D-erythritol synthase
            MEP cytidylyltransferase
              Short name=MCT
    2- Recommended name:
            2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
                Short name=MECPS
                Short name=MECDP-synthase
              EC=4.6.1.12

Gene names

Name:	ispDF
Ordered Locus Names:	CMM_2489

Organism

Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382) [Complete proteome] [HAMAP]

Taxonomic identifier

443906 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (ispD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (ispF) By similarity.
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520
Cofactor	Divalent metal cations By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Sequence similarities	In the N-terminal section; belongs to the ispD family. In the C-terminal section; belongs to the ispF family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding
Molecular function	Lyase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: HAMAP 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Bifunctional enzyme ispD/ispF HAMAP MF_01520		PRO_0000315552
Regions
	Region	1 – 257	257	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
	Region	258 – 410	153	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520
Sites
	Metal binding	264	1	Divalent metal cation By similarity
	Metal binding	266	1	Divalent metal cation By similarity
	Metal binding	298	1	Divalent metal cation By similarity
	Site	35	1	Transition state stabilizer By similarity
	Site	42	1	Transition state stabilizer By similarity
	Site	177	1	Positions MEP for the nucleophilic attack By similarity
	Site	230	1	Positions MEP for the nucleophilic attack By similarity
	Site	290	1	Transition state stabilizer By similarity
	Site	386	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5CTY4-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 987E4DA39C126EF3	FASTA	410	40,906
	10 20 30 40 50 60 MSHDPVVPSA PATDGGATDG PRLGVVVVAA GSGTRLGAGI PKALVEVGGV TLLARSLSSV 70 80 90 100 110 120 LGLAEEAHVV VVAPDTHLAE TSAVVDAVAG AARGSVAVVV GGATRQGSVR AGLAALVGSV 130 140 150 160 170 180 DTVLVHDAAR ALTPTDLFAA VARAVRAEGA GVVPGLPVTD TVKRVDPAGE CLGTVDRSDL 190 200 210 220 230 240 VGVQTPQGFP RAALDAAYAR AAAEHTDDAA LFQASGGRVR VIPGDALAFK VTTAWDLRRA 250 260 270 280 290 300 EELVARDAGA GSASSRLRSG IGTDVHAVDA SQPLWLAGLH WPGEAGLAGH SDGDAVSHAM 310 320 330 340 350 360 CDALLSAAGL GDIGGIFGTD DPELDGAHGE VFLRRTAELV RDAGYRIVNV AVQVMAVRPK 370 380 390 400 410 LSPRRAEAER ILSAAVGAPV SLAGTTTDGL GFTGRGDGVA AVATALVERL

References

[1]

"The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity." Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C. , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D. J. Bacteriol. 190:2138-2149(2008) [PubMed: 18192381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM711867 Genomic DNA. Translation: CAN02570.1.
RefSeq	YP_001223233.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5CTY4.
Genome annotation databases
GeneID	5174582.
GenomeReviews	Gene locus CMM_2489 in contig AM711867_GR.
KEGG	cmi:CMM_2489.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IVLIHDA.
Family and domain databases
HAMAP	MF_01520. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. IPR003526. MECDP_synthase_core. IPR020555. MECDP_synthase_CS. [Graphical view]
Pfam	PF01128. IspD. 1 hit. PF02542. YgbB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. ispD. 1 hit. TIGR00151. ispF. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. PS01350. ISPF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPDF_CLAM3

Accession

Primary (citable) accession number: A5CTY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)