A5CTL3 (ISPE_CLAM3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	CMM_2367

Organism

Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382) [Complete proteome] [HAMAP]

Taxonomic identifier

443906 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335706
Regions
	Nucleotide binding	104 – 114	11	ATP Potential
Sites
	Active site	18	1	By similarity
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5CTL3 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: D19E52C36CA51124	FASTA	312	32,061
	10 20 30 40 50 60 MTSAATTSDV VHARAPGKIN VSLTVGALQE DGYHDVATAY QAVGLYEDVW ATKADGFSVE 70 80 90 100 110 120 FGGSIDTSHL TTGADNLAVR AARLLARSTG YRGGVHLRIE KNVPIAGGMG GGSADAAATL 130 140 150 160 170 180 LACDTLWGTE RTRDQLLALG AELGADVPFA LAGGTAIGTG RGDRLSPALA KGTFQWVLAI 190 200 210 220 230 240 AEFGVSTPDV YGELDKHRER HAQDIFPAQQ IPQVDSGVLQ ALRAGDPHML AEVLHNDLQA 250 260 270 280 290 300 PALHLAPGLG EVLQLGEENG ALAGIVSGSG PTVAFLAADL DSALELQIAL SAARLTVIRA 310 TGPVHGARII TG

References

[1]

"The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity." Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C. , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D. J. Bacteriol. 190:2138-2149(2008) [PubMed: 18192381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCPPB 382.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM711867 Genomic DNA. Translation: CAN02442.1.
RefSeq	YP_001223112.1. NC_009480.1.
3D structure databases
ProteinModelPortal	A5CTL3.
ModBase	Search...
Protein-protein interaction databases
STRING	A5CTL3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5175414.
GenomeReviews	Gene locus CMM_2367 in contig AM711867_GR.
KEGG	cmi:CMM_2367.
PATRIC	21455545. VBIClaMic82482_2477.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	KINLHLG.
ProtClustDB	PRK03188.
Enzyme and pathway databases
BioCyc	CMIC443906:CMM_2367-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_CLAM3

Accession

Primary (citable) accession number: A5CTL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 12, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program