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A5CSW0 (THIM_CLAM3) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:CMM_2115
OrganismClavibacter michiganensis subsp. michiganensis (strain NCPPB 382) [Complete proteome] [HAMAP]
Taxonomic identifier443906 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter

Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 275275Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000383835

Sites

Binding site571Substrate; via amide nitrogen By similarity
Binding site1321ATP By similarity
Binding site1781ATP By similarity
Binding site2051Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A5CSW0 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 1570B23F0686569F

FASTA27527,500
        10         20         30         40         50         60 
MSAARPSAHE TTGTGTASAD LLLLLRERQP LVQCITNAVV TGFTANVLLA LGAAPAMTDV 

        70         80         90        100        110        120 
PTEAGPFARI ASGLLVNLGT PHAEQREAAV EAAHAAREAG TPWVLDPVAV GALPVRTRLA 

       130        140        150        160        170        180 
HELVALSPTV VRGNASEVIA LATGGAGGRG VDATDEVEAA LDAATLLART FGTVVAISGP 

       190        200        210        220        230        240 
VDHITDGHRV VRVHTGDALL TKVTGGGCAL GAVMAAFASL DPDPLRAAVA ATSVYTIAAE 

       250        260        270 
IAAEGASGPG SFAVGLLDAL DAVTPELVAQ RERLA 

« Hide

References

[1]"The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity."
Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C. expand/collapse author list , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D.
J. Bacteriol. 190:2138-2149(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCPPB 382.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM711867 Genomic DNA. Translation: CAN02184.1.
RefSeqYP_001222860.1. NC_009480.1.

3D structure databases

ProteinModelPortalA5CSW0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING443906.CMM_2115.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAN02184; CAN02184; CMM_2115.
GeneID5173647.
KEGGcmi:CMM_2115.
PATRIC21455067. VBIClaMic82482_2240.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMANACTQLA.

Enzyme and pathway databases

BioCycCMIC443906:GCI2-2182-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

Gene3D3.40.1190.20. 1 hit.
HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
SUPFAMSSF53613. SSF53613. 1 hit.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_CLAM3
AccessionPrimary (citable) accession number: A5CSW0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 12, 2007
Last modified: June 11, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways