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A5CSN9

- PNP_CLAM3

UniProt

A5CSN9 - PNP_CLAM3

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi525 – 5251MagnesiumUniRule annotation
    Metal bindingi531 – 5311MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciCMIC443906:GCI2-2109-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:CMM_2047
    OrganismiClavibacter michiganensis subsp. michiganensis (strain NCPPB 382)
    Taxonomic identifieri443906 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter
    ProteomesiUP000001564: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 757757Polyribonucleotide nucleotidyltransferasePRO_0000329592Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi443906.CMM_2047.

    Structurei

    3D structure databases

    ProteinModelPortaliA5CSN9.
    SMRiA5CSN9. Positions 5-728.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini591 – 65060KHUniRule annotationAdd
    BLAST
    Domaini662 – 73473S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218326.
    KOiK00962.
    OMAiTIAGKQD.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR014069. pppGpp_PNP.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46915. SSF46915. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    TIGR02696. pppGpp_PNP. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5CSN9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGPEIKFAE AVLDNGKYGT RTVRFEAGRL AQQAQGAVAA YLDEDTMLLS    50
    ATSVGKHPKD NFDFFPLTID VEERSYAAGK IPGSFFRREG RPSTEAILVC 100
    RLIDRPLRPS FITGLRNEVQ VVITVLSIAP DEFYDSLAIN AASASSMLSG 150
    IPFSGPIAGV RLALIGDQWV VFPKHSQLKE AVFDITVAGR VVTDSEGNED 200
    VAIMMVEAEA TEGAWDLIQG GATKPDEAIV AQGLEAAKPF IQQLVAAQAS 250
    LAQQAAKPTV DYPVFLPYAQ ETYDAVSALA LDELGTVYQT ADKIERQDAD 300
    DALKTRTKEA VAAKVEAGEL PQSALTEFSA AYKSVTKTVV RGRILRDGVR 350
    MDGRGLADIR PLDAEVQVIP RVHGSAIFQR GETQILGVTT LNMLKMEQQI 400
    DSLSPITKKR YLHHYNFPPY STGETGRVGS PKRREIGHGF LAERALVPVL 450
    PSREDFPYAI RQVSEALGSN GSTSMGSVCA STLSLLNAGV PLRAPVAGIA 500
    MGLVSDTVDG QVRYAALTDI LGAEDALGDM DFKVAGTSEF VTAIQLDTKL 550
    DGIPTSVLDG ALKQAKEART AILGVLNQAI DGPDEMAPTA PRVISVNIPV 600
    DKIGELIGPK GKTINAIQDE TGADISIEED GTVYIGAVDG PSADAARAQV 650
    NAIANPTNPE VGESFLGTVV KIATFGAFVS LLPGKDGLLH ISEVRKLAGG 700
    KRVENVEDVL GVGQKILVEI TKIDDRGKLS LAPVLEETAD QEGRDAASHG 750
    SEAPAEG 757
    Length:757
    Mass (Da):80,380
    Last modified:June 12, 2007 - v1
    Checksum:iDB68A6F09C72C334
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM711867 Genomic DNA. Translation: CAN02110.1.
    RefSeqiYP_001222789.1. NC_009480.1.

    Genome annotation databases

    EnsemblBacteriaiCAN02110; CAN02110; CMM_2047.
    GeneIDi5175509.
    KEGGicmi:CMM_2047.
    PATRICi21454921. VBIClaMic82482_2166.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM711867 Genomic DNA. Translation: CAN02110.1 .
    RefSeqi YP_001222789.1. NC_009480.1.

    3D structure databases

    ProteinModelPortali A5CSN9.
    SMRi A5CSN9. Positions 5-728.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 443906.CMM_2047.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAN02110 ; CAN02110 ; CMM_2047 .
    GeneIDi 5175509.
    KEGGi cmi:CMM_2047.
    PATRICi 21454921. VBIClaMic82482_2166.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218326.
    KOi K00962.
    OMAi TIAGKQD.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci CMIC443906:GCI2-2109-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR014069. pppGpp_PNP.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46915. SSF46915. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    TIGR02696. pppGpp_PNP. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity."
      Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C.
      , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D.
      J. Bacteriol. 190:2138-2149(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCPPB 382.

    Entry informationi

    Entry nameiPNP_CLAM3
    AccessioniPrimary (citable) accession number: A5CSN9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3