Reviewed, UniProtKB/Swiss-Prot A5CSL3 (DAPF_CLAM3)

Names and origin

Protein names

Recommended name:
    Diaminopimelate epimerase
      Short name=DAP epimerase
    EC=5.1.1.7

Gene names

Name:	dapF
Ordered Locus Names:	CMM_2021

Organism

Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382) [Complete proteome] [HAMAP]

Taxonomic identifier

443906 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP MF_00197
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-diaminopimelate from LL-diaminopimelate: step 1/1. HAMAP MF_00197
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the diaminopimelate epimerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysine biosynthetic process via diaminopimelate Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diaminopimelate epimerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Diaminopimelate epimerase HAMAP MF_00197		PRO_1000011870
Sites
	Active site	87	1	By similarity
	Active site	230	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5CSL3-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: B4B7E00A7FB0EBDF	FASTA	292	30,578
	10 20 30 40 50 60 MADLQFTKGQ GTGNDFVLFA DPAGEIDLTD TQVQALCDRH FGIGADGTIR AVLSTRIPEG 70 80 90 100 110 120 RAALEEDPDA EWFMDYRNVD GSPAEMCGNG IRVFTLFLIE NGLIELPPGR TIPIGTRAGV 130 140 150 160 170 180 RDVQRSGSGF QVDLGRWELA GGEPLVRAKD LQVARPGLGI DVGNPHVVVA LSSEDELAEA 190 200 210 220 230 240 DLAFVPQLDP EPAAGANVEL VVPADPLVVD GVGHITMRVH ERGSGETLSC GTGAAAAALA 250 260 270 280 290 TRHWAGAAAP HQWRVQLPGG ALGVRMFPTE DGEHVGLSGP AELVFDGVVA LA

References

[1]

"The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity." Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C. , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D. J. Bacteriol. 190:2138-2149(2008) [PubMed: 18192381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM711867 Genomic DNA. Translation: CAN02084.1.
RefSeq	YP_001222763.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5174176.
GenomeReviews	Gene locus CMM_2021 in contig AM711867_GR.
KEGG	cmi:CMM_2021.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A5CSL3. PDAEWFM.
Family and domain databases
HAMAP	MF_00197. [Tree]
InterPro	IPR001653. DAP_epimerase. IPR018510. DAP_epimerase_CS. [Graphical view]
Pfam	PF01678. DAP_epimerase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00652. DapF. 1 hit.
PROSITE	PS01326. DAP_EPIMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPF_CLAM3

Accession

Primary (citable) accession number: A5CSL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	June 16, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)