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A5CSL3

- DAPF_CLAM3

UniProt

A5CSL3 - DAPF_CLAM3

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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141SubstrateUniRule annotation
Binding sitei78 – 781SubstrateUniRule annotation
Active sitei87 – 871Proton donor/acceptorUniRule annotation
Binding sitei164 – 1641SubstrateUniRule annotation
Sitei166 – 1661Important for catalytic activityUniRule annotation
Binding sitei197 – 1971SubstrateUniRule annotation
Sitei221 – 2211Important for catalytic activityUniRule annotation
Active sitei230 – 2301Proton donor/acceptorUniRule annotation

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciCMIC443906:GCI2-2083-MONOMER.
UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:CMM_2021
OrganismiClavibacter michiganensis subsp. michiganensis (strain NCPPB 382)
Taxonomic identifieri443906 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter
ProteomesiUP000001564: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292Diaminopimelate epimerasePRO_1000011870Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi87 ↔ 230

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi443906.CMM_2021.

Structurei

3D structure databases

ProteinModelPortaliA5CSL3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 893Substrate bindingUniRule annotation
Regioni221 – 2222Substrate bindingUniRule annotation
Regioni231 – 2322Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0253.
HOGENOMiHOG000220467.
KOiK01778.
OMAiITIEMGT.
OrthoDBiEOG6ND0M5.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5CSL3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADLQFTKGQ GTGNDFVLFA DPAGEIDLTD TQVQALCDRH FGIGADGTIR
60 70 80 90 100
AVLSTRIPEG RAALEEDPDA EWFMDYRNVD GSPAEMCGNG IRVFTLFLIE
110 120 130 140 150
NGLIELPPGR TIPIGTRAGV RDVQRSGSGF QVDLGRWELA GGEPLVRAKD
160 170 180 190 200
LQVARPGLGI DVGNPHVVVA LSSEDELAEA DLAFVPQLDP EPAAGANVEL
210 220 230 240 250
VVPADPLVVD GVGHITMRVH ERGSGETLSC GTGAAAAALA TRHWAGAAAP
260 270 280 290
HQWRVQLPGG ALGVRMFPTE DGEHVGLSGP AELVFDGVVA LA
Length:292
Mass (Da):30,578
Last modified:June 12, 2007 - v1
Checksum:iB4B7E00A7FB0EBDF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM711867 Genomic DNA. Translation: CAN02084.1.
RefSeqiWP_012038708.1. NC_009480.1.
YP_001222763.1. NC_009480.1.

Genome annotation databases

EnsemblBacteriaiCAN02084; CAN02084; CMM_2021.
GeneIDi5174176.
KEGGicmi:CMM_2021.
PATRICi21454871. VBIClaMic82482_2141.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM711867 Genomic DNA. Translation: CAN02084.1 .
RefSeqi WP_012038708.1. NC_009480.1.
YP_001222763.1. NC_009480.1.

3D structure databases

ProteinModelPortali A5CSL3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 443906.CMM_2021.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAN02084 ; CAN02084 ; CMM_2021 .
GeneIDi 5174176.
KEGGi cmi:CMM_2021.
PATRICi 21454871. VBIClaMic82482_2141.

Phylogenomic databases

eggNOGi COG0253.
HOGENOMi HOG000220467.
KOi K01778.
OMAi ITIEMGT.
OrthoDBi EOG6ND0M5.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .
BioCyci CMIC443906:GCI2-2083-MONOMER.

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity."
    Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C.
    , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D.
    J. Bacteriol. 190:2138-2149(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCPPB 382.

Entry informationi

Entry nameiDAPF_CLAM3
AccessioniPrimary (citable) accession number: A5CSL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: October 29, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3