A5CS59 (RSMH_CLAM3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	CMM_1867

Organism

Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382) [Complete proteome] [HAMAP]

Taxonomic identifier

443906 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000386807
Regions
	Region	38 – 40	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	57	1	S-adenosyl-L-methionine By similarity
	Binding site	91	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	105	1	S-adenosyl-L-methionine By similarity
	Binding site	112	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5CS59 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 8FCC3D56FAA4455C	FASTA	318	34,752
	10 20 30 40 50 60 MALDDIHTPV LLERCLELLA PALQGEGAVL VDATLGMAGH SEAFLDALPG LRLVGLDRDP 70 80 90 100 110 120 DALAIAGERL ARFGDRVNLV HTVYDGIGRA LDGLGIGEVQ GVFFDLGVSS LQLDRVERGF 130 140 150 160 170 180 SYSQDAPLDM RMDGTAGLTA AQVVAEYDEL ELRRIFYDYG EEKLAPRYAS RIVQAREVEP 190 200 210 220 230 240 ITTSARLVEI IQQATPAAVQ RAGHPAKRVF QALRIEVNQE LSVLARAMPA AVDRLAVGGR 250 260 270 280 290 300 VVVESYQSLE DRIVKRELRA RSTSTAPVGL PVELPEHRPE LKLLVRGAEL ADQHEIAQNP 310 RAASVRLRAA ERARRRHA

References

[1]

"The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity." Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C. , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D. J. Bacteriol. 190:2138-2149(2008) [PubMed: 18192381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCPPB 382.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM711867 Genomic DNA. Translation: CAN01923.1.
RefSeq	YP_001222609.1. NC_009480.1.
3D structure databases
ProteinModelPortal	A5CS59.
ModBase	Search...
Protein-protein interaction databases
STRING	A5CS59.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5174097.
GenomeReviews	Gene locus CMM_1867 in contig AM711867_GR.
KEGG	cmi:CMM_1867.
PATRIC	21454559. VBIClaMic82482_1989.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0275.
HOGENOM	HBG302779.
OMA	NPATRAF.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	CMIC443906:CMM_1867-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_CLAM3

Accession

Primary (citable) accession number: A5CS59

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	June 12, 2007
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program