A5CS23 (GLPK_CLAM3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	CMM_1831

Organism

Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382) [Complete proteome] [HAMAP]

Taxonomic identifier

443906 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism By similarity. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Glycerol kinase HAMAP MF_00186		PRO_1000124187
Regions
	Nucleotide binding	414 – 418	5	ATP By similarity
Sites
	Binding site	13	1	Substrate By similarity
	Binding site	17	1	ATP By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	247	1	Substrate By similarity
	Binding site	269	1	ATP By similarity
	Binding site	313	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5CS23 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 406E5B6195561AEB	FASTA	505	55,485
	10 20 30 40 50 60 MSEKYIVAID QGTTSTRAIV FDHSGSIVST GQLEHEQIFP RAGWVEHDPM EIWRNTREVI 70 80 90 100 110 120 GQALSKADIT RHDVEAVGIT NQRETAVVWD RTTGKPVYNA IVWQDTRTQK IVDRLAADGG 130 140 150 160 170 180 VERFKPTVGL PLATYFSGTK IVWILENVDG AREKAEAGEL MFGTTDTWVL WNLTGGTDGG 190 200 210 220 230 240 VHVTDVTNAS RTLFMDLETL QWDDEILKAF DVPRSMLPEI KSSSEVYGQV ESSSLLREVP 250 260 270 280 290 300 IAGILGDQQA ATFGQAAFDQ GESKNTYGTG NFLIFNTGTD IIHSQNGLLT TLGYKLGDQE 310 320 330 340 350 360 PHYALEGSIA VTGSLVQWMR DNLGLVSSAA EIETLAATVE DNGGVYFVPA FSGLFAPYWR 370 380 390 400 410 420 SDARGALVGL TRYVNKGHIA RAALEATAFQ TREVLDAVNA DSGVDLTELK VDGGMIANNL 430 440 450 460 470 480 LMQFQADILG VPVVRPVVAE TTALGAAYAA GLAVGFWKDL DDLRQNWQED SRWTPDMDDA 490 500 ERERQLRLWK KAVTKTFDWV DDDVQ

References

[1]

"The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity." Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C. , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D. J. Bacteriol. 190:2138-2149(2008) [PubMed: 18192381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCPPB 382.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM711867 Genomic DNA. Translation: CAN01886.1.
RefSeq	YP_001222573.1. NC_009480.1.
3D structure databases
ProteinModelPortal	A5CS23.
ModBase	Search...
Protein-protein interaction databases
STRING	A5CS23.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5175907.
GenomeReviews	Gene locus CMM_1831 in contig AM711867_GR.
KEGG	cmi:CMM_1831.
PATRIC	21454483. VBIClaMic82482_1951.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0554.
HOGENOM	HBG511469.
OMA	ATYFSGT.
ProtClustDB	CLSK928226.
Enzyme and pathway databases
BioCyc	CMIC443906:CMM_1831-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_CLAM3

Accession

Primary (citable) accession number: A5CS23

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	June 12, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program