A5CRX9 (PYRB_CLAM3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	CMM_1786

Organism

Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382) [Complete proteome] [HAMAP]

Taxonomic identifier

443906 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_1000000006

Sequences

	Sequence		Length	Mass (Da)
	A5CRX9 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: AD68D4A65BA11ACF	FASTA	318	33,998
	10 20 30 40 50 60 MRHLLSTRDL SRDEAVHILD VAEDMADVGT REIKKTPALR GRTVVNLFFE DSTRTRISFE 70 80 90 100 110 120 AAAKRLSADV INFSAKGSSV SKGESLKDTA QTLQAMGADG VVVRHPSSGA PHTLAGSGWI 130 140 150 160 170 180 DAGIVNAGDG THEHPTQALL DAFTIRRRLH GTAARGKGLD GTRVVIVGDV LHSRVARSNA 190 200 210 220 230 240 WLLTTLGAEV TLVAPPTLVP VGVGSWPVTV RYDLDAALAE GKPDAVMMLR IQAERMRAAF 250 260 270 280 290 300 FPNPCEYARI WGLDDARLAL LGPDTIVMHP GPMNRGLEIS AAAADSERST VREQVANGVS 310 VRMAVLYLLL SGDGKADR

References

[1]

"The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity." Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C. , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D. J. Bacteriol. 190:2138-2149(2008) [PubMed: 18192381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCPPB 382.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM711867 Genomic DNA. Translation: CAN01842.1.
RefSeq	YP_001222529.1. NC_009480.1.
3D structure databases
ProteinModelPortal	A5CRX9.
ModBase	Search...
Protein-protein interaction databases
STRING	A5CRX9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5173363.
GenomeReviews	Gene locus CMM_1786 in contig AM711867_GR.
KEGG	cmi:CMM_1786.
PATRIC	21454385. VBIClaMic82482_1906.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0540.
HOGENOM	HBG579429.
OMA	LQRERMS.
ProtClustDB	PRK00856.
Enzyme and pathway databases
BioCyc	CMIC443906:CMM_1786-MONOMER.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K00609.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_CLAM3

Accession

Primary (citable) accession number: A5CRX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program