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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45ZincUniRule annotation1
Binding sitei60Cysteinyl adenylateUniRule annotation1
Binding sitei239Cysteinyl adenylateUniRule annotation1
Metal bindingi243ZincUniRule annotation1
Metal bindingi268ZincUniRule annotation1
Binding sitei294Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseUniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligaseUniRule annotation
Alternative name(s):
Mycothiol ligaseUniRule annotation
Short name:
MSH ligaseUniRule annotation
Gene namesi
Name:mshCUniRule annotation
Ordered Locus Names:CMM_1694
OrganismiClavibacter michiganensis subsp. michiganensis (strain NCPPB 382)
Taxonomic identifieri443906 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrobacteriaceaeClavibacter
Proteomesi
  • UP000001564 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004004351 – 426L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseAdd BLAST426

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi443906.CMM_1694.

Structurei

3D structure databases

ProteinModelPortaliA5CRN7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni45 – 48Cysteinyl adenylate bindingUniRule annotation4
Regioni83 – 85Cysteinyl adenylate bindingUniRule annotation3
Regioni261 – 263Cysteinyl adenylate bindingUniRule annotation3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi47 – 57"HIGH" regionUniRule annotationAdd BLAST11
Motifi199 – 204"ERGGDP" regionUniRule annotation6
Motifi300 – 304"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiMARIYVC.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.

Sequencei

Sequence statusi: Complete.

A5CRN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAWPRPVVP AVAGEPPVPT LFDTSAGSVR PAECTGDGRV GLYVCGITPY
60 70 80 90 100
DATHIGHAST YLAFDTLQRV WLDRGYDVAY VQNVTDVDDP LLERATATGV
110 120 130 140 150
DWRDLAAEQV ELFRTDMEAL RILPPDSYVG VTEVVDEVAA AVAELVRRGT
160 170 180 190 200
AYPVATPDAA QPGARDLYFD VARAGADGPW ALGDESGYDR DTMAALSAER
210 220 230 240 250
GGDPERPGKR DPLDPLLWRA ERADEPAWDS AVGRGRPGWH IECAVIALRK
260 270 280 290 300
LDRPVTVQGG GSDLIFPHHE MSAGHAAALT GEDFACVYAH SGMVAYQGEK
310 320 330 340 350
MSKSLGNLVL VSRLRAAGVD PRAIRLALLA QHYRADWEWT DALLAESVAR
360 370 380 390 400
LAAWDAWAAD AASTDADAGE PGELVQLVRE RLADDLDTPG AILLLDLRVA
410 420
TGVPATPVEV AAVDALLGVA LGSPAA
Length:426
Mass (Da):45,369
Last modified:June 12, 2007 - v1
Checksum:i7D34D4BDC3D7C1D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM711867 Genomic DNA. Translation: CAN01747.1.
RefSeqiWP_012038382.1. NC_009480.1.

Genome annotation databases

EnsemblBacteriaiCAN01747; CAN01747; CMM_1694.
KEGGicmi:CMM_1694.
PATRICi21454193. VBIClaMic82482_1812.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM711867 Genomic DNA. Translation: CAN01747.1.
RefSeqiWP_012038382.1. NC_009480.1.

3D structure databases

ProteinModelPortaliA5CRN7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi443906.CMM_1694.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAN01747; CAN01747; CMM_1694.
KEGGicmi:CMM_1694.
PATRICi21454193. VBIClaMic82482_1812.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiMARIYVC.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHC_CLAM3
AccessioniPrimary (citable) accession number: A5CRN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: June 12, 2007
Last modified: November 2, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.