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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei618 – 6181ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCMIC443906:GCI2-1594-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:CMM_1547
OrganismiClavibacter michiganensis subsp. michiganensis (strain NCPPB 382)
Taxonomic identifieri443906 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrobacteriaceaeClavibacter
ProteomesiUP000001564 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 851851Leucine--tRNA ligasePRO_0000334743Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi443906.CMM_1547.

Structurei

3D structure databases

ProteinModelPortaliA5CR89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi51 – 6111"HIGH" regionAdd
BLAST
Motifi615 – 6195"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiLHMGHAE.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5CR89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHETPDTPG ETYDFRAIEA EWSEVWEREQ PFRTPDASDS RPRKYILDMF
60 70 80 90 100
PYPSGDLHMG HAEAFALGDA VARYWRQQGF NVLHPIGWDS FGLPAENAAI
110 120 130 140 150
KRGVDPREWT YANIETQKQS MKRYGLSFDW ERELHTSDPE YYRWNQWLFL
160 170 180 190 200
KMHEKGLAYR KDSWVNWDPV DQTVLANEQV LPDGTSDRSG AVVVKKKLTQ
210 220 230 240 250
WYLRITDYAD RLVDDLNQLE GTWPAKVISM QRNWIGRSIG AEVDFVVEGR
260 270 280 290 300
DEPVTVFTTR PDTLHGATFM VVAPDSDLAA ELVEGASDEV RERFRGYLER
310 320 330 340 350
TQRLNEIERS TTDRPKTGIP LGRTAINPVN GERIPVWAAD YLLADYGTGA
360 370 380 390 400
VMAVPAHDQR DLDFARAFDL PVRVVVDTTQ PVTGAIRIIP EDGELPDLEE
410 420 430 440 450
VLPGRTGVAL PGEGRLINSG SLNGLSKQPA IKRVIEQLEA EGRGRAAKNY
460 470 480 490 500
RLRDWLISRQ RFWGTPIPIV YDAEGNEIRV PEDQLPVRLP DTEGLDLAPK
510 520 530 540 550
GKSPLAAATE WTNVPSPVDG SPATRDPDTM DTFMDSSWYW LRFLSPNDAT
560 570 580 590 600
KAFDPADADR WAPIDQYVGG VEHAILHLLY SRFITKVLFD LGYVTFTEPF
610 620 630 640 650
SALLNQGMVL SGGSKMSKSK GGVDLGSEMD RHGVDAIRLT MAFAGPPEDD
660 670 680 690 700
IDWEDVSPSG SAKFLARAWR LTGDITSAPE IEWKTGDEAL RRVTHRFLAE
710 720 730 740 750
APGMLEAFKF NVVIARTMEL VNAIRKTIDQ GPGGGDAAVR EATEVVAIAL
760 770 780 790 800
SLFAPYTAED MWRRLGREGS VAFAGWRKAE RNLLVQSTVT AVVQVDGKVR
810 820 830 840 850
DKLEVDAKIG ADELEALARE TAGVKRSTAG RTIDKVIVRA PKIVSITTTA

P
Length:851
Mass (Da):94,950
Last modified:June 12, 2007 - v1
Checksum:i42B1FC0B7736F1B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM711867 Genomic DNA. Translation: CAN01594.1.
RefSeqiWP_012038234.1. NC_009480.1.
YP_001222289.1. NC_009480.1.

Genome annotation databases

EnsemblBacteriaiCAN01594; CAN01594; CMM_1547.
KEGGicmi:CMM_1547.
PATRICi21453887. VBIClaMic82482_1662.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM711867 Genomic DNA. Translation: CAN01594.1.
RefSeqiWP_012038234.1. NC_009480.1.
YP_001222289.1. NC_009480.1.

3D structure databases

ProteinModelPortaliA5CR89.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi443906.CMM_1547.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAN01594; CAN01594; CMM_1547.
KEGGicmi:CMM_1547.
PATRICi21453887. VBIClaMic82482_1662.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiLHMGHAE.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCMIC443906:GCI2-1594-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity."
    Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C.
    , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D.
    J. Bacteriol. 190:2138-2149(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCPPB 382.

Entry informationi

Entry nameiSYL_CLAM3
AccessioniPrimary (citable) accession number: A5CR89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: June 24, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.