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A5CR89 (SYL_CLAM3) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CMM_1547
OrganismClavibacter michiganensis subsp. michiganensis (strain NCPPB 382) [Complete proteome] [HAMAP]
Taxonomic identifier443906 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter

Protein attributes

Sequence length851 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

regulation of translational fidelity

Inferred from electronic annotation. Source: GOC

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 851851Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334743

Regions

Motif51 – 6111"HIGH" region HAMAP-Rule MF_00049
Motif615 – 6195"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6181ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5CR89 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 42B1FC0B7736F1B6

FASTA85194,950
        10         20         30         40         50         60 
MAHETPDTPG ETYDFRAIEA EWSEVWEREQ PFRTPDASDS RPRKYILDMF PYPSGDLHMG 

        70         80         90        100        110        120 
HAEAFALGDA VARYWRQQGF NVLHPIGWDS FGLPAENAAI KRGVDPREWT YANIETQKQS 

       130        140        150        160        170        180 
MKRYGLSFDW ERELHTSDPE YYRWNQWLFL KMHEKGLAYR KDSWVNWDPV DQTVLANEQV 

       190        200        210        220        230        240 
LPDGTSDRSG AVVVKKKLTQ WYLRITDYAD RLVDDLNQLE GTWPAKVISM QRNWIGRSIG 

       250        260        270        280        290        300 
AEVDFVVEGR DEPVTVFTTR PDTLHGATFM VVAPDSDLAA ELVEGASDEV RERFRGYLER 

       310        320        330        340        350        360 
TQRLNEIERS TTDRPKTGIP LGRTAINPVN GERIPVWAAD YLLADYGTGA VMAVPAHDQR 

       370        380        390        400        410        420 
DLDFARAFDL PVRVVVDTTQ PVTGAIRIIP EDGELPDLEE VLPGRTGVAL PGEGRLINSG 

       430        440        450        460        470        480 
SLNGLSKQPA IKRVIEQLEA EGRGRAAKNY RLRDWLISRQ RFWGTPIPIV YDAEGNEIRV 

       490        500        510        520        530        540 
PEDQLPVRLP DTEGLDLAPK GKSPLAAATE WTNVPSPVDG SPATRDPDTM DTFMDSSWYW 

       550        560        570        580        590        600 
LRFLSPNDAT KAFDPADADR WAPIDQYVGG VEHAILHLLY SRFITKVLFD LGYVTFTEPF 

       610        620        630        640        650        660 
SALLNQGMVL SGGSKMSKSK GGVDLGSEMD RHGVDAIRLT MAFAGPPEDD IDWEDVSPSG 

       670        680        690        700        710        720 
SAKFLARAWR LTGDITSAPE IEWKTGDEAL RRVTHRFLAE APGMLEAFKF NVVIARTMEL 

       730        740        750        760        770        780 
VNAIRKTIDQ GPGGGDAAVR EATEVVAIAL SLFAPYTAED MWRRLGREGS VAFAGWRKAE 

       790        800        810        820        830        840 
RNLLVQSTVT AVVQVDGKVR DKLEVDAKIG ADELEALARE TAGVKRSTAG RTIDKVIVRA 

       850 
PKIVSITTTA P 

« Hide

References

[1]"The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity."
Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C. expand/collapse author list , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D.
J. Bacteriol. 190:2138-2149(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCPPB 382.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM711867 Genomic DNA. Translation: CAN01594.1.
RefSeqYP_001222289.1. NC_009480.1.

3D structure databases

ProteinModelPortalA5CR89.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING443906.CMM_1547.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAN01594; CAN01594; CMM_1547.
GeneID5174696.
KEGGcmi:CMM_1547.
PATRIC21453887. VBIClaMic82482_1662.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMALHMGHAE.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCMIC443906:GCI2-1594-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CLAM3
AccessionPrimary (citable) accession number: A5CR89
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries