A5CQX1 (A5CQX1_CLAM3) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Name:	treZ EMBL CAN01475.1
Ordered Locus Names:	CMM_1430

Organism

Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382) [Complete proteome] [HAMAP] EMBL CAN01475.1

Taxonomic identifier

443906 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 EMBL CAN01475.1 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	252	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	289	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	385	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	A5CQX1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 6C47BF54381F2BF3	FASTA	600	65,853
	10 20 30 40 50 60 MTDDRFDIWA PKARTLALSV GDERLPLSPV GDGWWTLDAD RAEALPSGDL DYGYLVDDAE 70 80 90 100 110 120 TPLPDPRSRR QPEGVHGRSR TYDPSSFAWT DQAWTGRQLA GAVIYEMHIG TFTPDGTLDS 130 140 150 160 170 180 AIDRLDHLVA LGVDLVEVLP VNGFNGTHNW GYDGVLWYTV QETYGGPEAY QRFVDACHAR 190 200 210 220 230 240 GLGVVQDVVY NHLGPSGNYL PVYGPYLHEA SANTWGSSLN LDGEDSGPVR EYIIDNALMW 250 260 270 280 290 300 LGDYHVDALR LDAVHALVDD TATHLLEELA VQVDVLSAHV GRPLTLIAES DLNDPKLITS 310 320 330 340 350 360 REAHGYGLDA QWSDDFHHAV HVALTGETTG YYEDFASLGA LAKVITRGFF HDGTWSSFRG 370 380 390 400 410 420 RVHGRPLDTE RIPAHRLVVA NQNHDQIGNR ATGDRLTATL DEGGLALAAV LTLTSPFTPM 430 440 450 460 470 480 LFMGEEWGAT TSWQFFTSHP EHDLGEATAK GRIAEFAKMG WDESVVPNPQ DLSTFQDSKL 490 500 510 520 530 540 DWSELYGTEA AESQHARLFS LYSELIRLRR AHPDLTDPRF AEVEVEVHEE ARLLVMDRGE 550 560 570 580 590 600 LSIVVNLSDE ERRVPVVGER PALLLATAPG VALGDDEVVL PARSAAILGP VADSAEALLA

References

[1]

"The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity." Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C. , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D. J. Bacteriol. 190:2138-2149(2008) [PubMed: 18192381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM711867 Genomic DNA. Translation: CAN01475.1.
RefSeq	YP_001222171.1. NC_009480.1.
3D structure databases
ProteinModelPortal	A5CQX1.
ModBase	Search...
Protein-protein interaction databases
STRING	A5CQX1.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5172960.
GenomeReviews	Gene locus CMM_1430 in contig AM711867_GR.
KEGG	cmi:CMM_1430.
PATRIC	21453633. VBIClaMic82482_1540.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG367595.
OMA	EGFVYQG.
ProtClustDB	CLSK2320911.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR022567. Maltooligo_trehalose_bac_C. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. PF11941. DUF3459. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A5CQX1_CLAM3

Accession

Primary (citable) accession number: A5CQX1

Entry history

Integrated into UniProtKB/TrEMBL:	June 12, 2007
Last sequence update:	June 12, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)