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A5CQ99

- LIPA_CLAM3

UniProt

A5CQ99 - LIPA_CLAM3

Protein

Lipoyl synthase

Gene

lipA

Organism
Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi57 – 571Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi83 – 831Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi87 – 871Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi90 – 901Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciCMIC443906:GCI2-1246-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:CMM_1208
    OrganismiClavibacter michiganensis subsp. michiganensis (strain NCPPB 382)
    Taxonomic identifieri443906 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter
    ProteomesiUP000001564: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 331331Lipoyl synthasePRO_0000325239Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi443906.CMM_1208.

    Structurei

    3D structure databases

    ProteinModelPortaliA5CQ99.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiEWLRRPI.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A5CQ99-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAATAPDGR RMLRLEVRNA ETPIERKPGW IKTTARMGPE YQALQQLVKT    50
    EDLHTVCQEA ACPNIYECWE DREATFLIGG SQCTRRCDFC QIDTGKPADY 100
    DTDEPRRVAD SVRRMGLRYA TVTGVARDDL PDEGAWLHAE TVRRIHADNP 150
    GTGVEILATD FSGNPDLLAE VFSSRPEVFA HNVETVPRIF KRIRPAFRYE 200
    RSLDVITQAR DAGLITKSNL ILGMGETRDE VSEALVDLHD AGCDIITVTQ 250
    YLRPSPRHLP VARWVRPEEF VEIKAEAEAI GFLGVLAGPL VRSSYRAGRL 300
    YAQSMRAKGR ELPEGLAHLA DPANGFAQAV G 331
    Length:331
    Mass (Da):36,800
    Last modified:June 12, 2007 - v1
    Checksum:i7C6EF3DE8174B07C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM711867 Genomic DNA. Translation: CAN01252.1.
    RefSeqiYP_001221949.1. NC_009480.1.

    Genome annotation databases

    EnsemblBacteriaiCAN01252; CAN01252; CMM_1208.
    GeneIDi5174720.
    KEGGicmi:CMM_1208.
    PATRICi21453177. VBIClaMic82482_1313.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM711867 Genomic DNA. Translation: CAN01252.1 .
    RefSeqi YP_001221949.1. NC_009480.1.

    3D structure databases

    ProteinModelPortali A5CQ99.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 443906.CMM_1208.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAN01252 ; CAN01252 ; CMM_1208 .
    GeneIDi 5174720.
    KEGGi cmi:CMM_1208.
    PATRICi 21453177. VBIClaMic82482_1313.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi EWLRRPI.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci CMIC443906:GCI2-1246-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity."
      Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C.
      , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D.
      J. Bacteriol. 190:2138-2149(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCPPB 382.

    Entry informationi

    Entry nameiLIPA_CLAM3
    AccessioniPrimary (citable) accession number: A5CQ99
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3