A5CQ46 (KHSE_CLAM3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	CMM_1155

Organism

Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382) [Complete proteome] [HAMAP]

Taxonomic identifier

443906 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Homoserine kinase HAMAP MF_00384		PRO_1000080118
Regions
	Nucleotide binding	96 – 106	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A5CQ46 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: B5A8BB4FB7DD7B97	FASTA	316	32,631
	10 20 30 40 50 60 MRSALATGRR VQVRVPATSA NLGPGFDTLG LALALYDDLT VTVRDAPGAT VDVRGVGAGE 70 80 90 100 110 120 VPTDETNLVV TAIAHTFAAF DQPMPGLDLV AENRIPHGRG LGSSGAAIVS GIMAAQGLLA 130 140 150 160 170 180 GTVEIDADAL LRLATEMEGH PDNVAPALFG GLTIAWVDGQ GPQHKKLAVH RGVSPLVLVP 190 200 210 220 230 240 VATMSTALAR SLQPESVPHE DAIFNVSRSA LLIAALIQSP ELLLAATEDR LHQDYRAAAM 250 260 270 280 290 300 PETNELVHLL RERGYAAVVS GAGPSLLVLG SDPGQRLTAA ELVAERSTNP WTALMLAVDV 310 KGSTVQVVDE GSAPAA

References

[1]

"The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity." Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C. , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D. J. Bacteriol. 190:2138-2149(2008) [PubMed: 18192381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCPPB 382.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM711867 Genomic DNA. Translation: CAN01199.1.
RefSeq	YP_001221896.1. NC_009480.1.
3D structure databases
ProteinModelPortal	A5CQ46.
ModBase	Search...
Protein-protein interaction databases
STRING	A5CQ46.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5174857.
GenomeReviews	Gene locus CMM_1155 in contig AM711867_GR.
KEGG	cmi:CMM_1155.
PATRIC	21453063. VBIClaMic82482_1256.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0083.
HOGENOM	HBG646290.
OMA	DRLHQPY.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	CMIC443906:CMM_1155-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_CLAM3

Accession

Primary (citable) accession number: A5CQ46

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 12, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program