Reviewed, UniProtKB/Swiss-Prot A5CPZ4 (LEU3_CLAM3)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:	leuB
Ordered Locus Names:	CMM_1104

Organism

Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382) [Complete proteome] [HAMAP]

Taxonomic identifier

443906 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate By similarity.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	3-isopropylmalate dehydrogenase		PRO_1000063869
Regions
	Nucleotide binding	283 – 295	13	NAD By similarity
Sites
	Metal binding	223	1	Magnesium or manganese By similarity
	Metal binding	247	1	Magnesium or manganese By similarity
	Metal binding	251	1	Magnesium or manganese By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	108	1	Substrate By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	223	1	Substrate By similarity
	Site	139	1	Important for catalysis By similarity
	Site	190	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5CPZ4-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: E2BD12E685D06224	FASTA	355	37,179
	10 20 30 40 50 60 MPRTISLAVV PGDGIGPEVV HEALRVLREA VPADVSLDTT QYPFGAGHFL ETGEILTDSD 70 80 90 100 110 120 LAALAQHDAI LLGAVGGDPR DARLAGGIIE RGLLLKLRFA FDHYINLRPT TLLPGVTSPL 130 140 150 160 170 180 ASPGEVDFVV VREGTEGPYA GNGGVLRRGT EHEIATEVSV NTAHGVERTV RFAFDLASKR 190 200 210 220 230 240 ERKRVTLVHK TNVLTFAGSL WQRTVDRVAA EHPDVAVDYL HVDATMIFLV TDPSRFDVIV 250 260 270 280 290 300 SDNLFGDIIT DLAAAISGGI GLAASGNVNP TGAFPSMFEP VHGSAPDIAG QQKADPTAAI 310 320 330 340 350 LSVSLLLDHL GLPEAAARVT AAVSADLAAR AAGDPAPRST AEVGDAVIRA LSTNH

References

[1]

"The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity." Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M., Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C. , Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O., Bartels D. J. Bacteriol. 190:2138-2149(2008) [PubMed: 18192381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM711867 Genomic DNA. Translation: CAN01147.1.
RefSeq	YP_001221845.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5175261.
GenomeReviews	Gene locus CMM_1104 in contig AM711867_GR.
KEGG	cmi:CMM_1104.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01035. [Tree]
InterPro	IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_CLAM3

Accession

Primary (citable) accession number: A5CPZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 12, 2007
Last modified:	November 4, 2008
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)