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Protein

Ketol-acid reductoisomerase (NADP(+))

Gene

ilvC

Organism
Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.UniRule annotation

Catalytic activityi

(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.UniRule annotation
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvB)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvB)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei48NADPUniRule annotation1
Binding sitei51NADPUniRule annotation1
Binding sitei53NADPUniRule annotation1
Active sitei108UniRule annotation1
Binding sitei134NADP; via amide nitrogenUniRule annotation1
Metal bindingi191Magnesium 1UniRule annotation1
Metal bindingi191Magnesium 2UniRule annotation1
Metal bindingi195Magnesium 1UniRule annotation1
Metal bindingi227Magnesium 2UniRule annotation1
Metal bindingi231Magnesium 2UniRule annotation1
Binding sitei252SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 28NADPUniRule annotation4
Nucleotide bindingi83 – 86NADPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
LigandMagnesium, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00047; UER00056
UPA00049; UER00060

Names & Taxonomyi

Protein namesi
Recommended name:
Ketol-acid reductoisomerase (NADP(+))UniRule annotation (EC:1.1.1.86UniRule annotation)
Short name:
KARIUniRule annotation
Alternative name(s):
Acetohydroxy-acid isomeroreductaseUniRule annotation
Short name:
AHIRUniRule annotation
Alpha-keto-beta-hydroxylacyl reductoisomeraseUniRule annotation
Ketol-acid reductoisomerase type 1UniRule annotation
Ketol-acid reductoisomerase type IUniRule annotation
Gene namesi
Name:ilvCUniRule annotation
Ordered Locus Names:CMM_1096
OrganismiClavibacter michiganensis subsp. michiganensis (strain NCPPB 382)
Taxonomic identifieri443906 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrobacteriaceaeClavibacter
Proteomesi
  • UP000001564 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000505011 – 341Ketol-acid reductoisomerase (NADP(+))Add BLAST341

Interactioni

Protein-protein interaction databases

STRINGi443906.CMM_1096

Structurei

3D structure databases

ProteinModelPortaliA5CPY6
SMRiA5CPY6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 182KARI N-terminal RossmannPROSITE-ProRule annotationAdd BLAST181
Domaini183 – 328KARI C-terminal knottedPROSITE-ProRule annotationAdd BLAST146

Sequence similaritiesi

Belongs to the ketol-acid reductoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6M Bacteria
COG0059 LUCA
HOGENOMiHOG000016230
KOiK00053
OMAiRAMFSWL

Family and domain databases

HAMAPiMF_00435 IlvC, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013023 KARI
IPR000506 KARI_C
IPR013116 KARI_N
IPR014359 KARI_prok
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR21371 PTHR21371, 1 hit
PfamiView protein in Pfam
PF01450 IlvC, 1 hit
PF07991 IlvN, 1 hit
PIRSFiPIRSF000116 IlvC_gammaproteo, 1 hit
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00465 ilvC, 1 hit
PROSITEiView protein in PROSITE
PS51851 KARI_C, 1 hit
PS51850 KARI_N, 1 hit

Sequencei

Sequence statusi: Complete.

A5CPY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDIVYDKDA DLSLIQGRKV AVIGYGSQGH AHALNLRDSG VEVVIGLKEG
60 70 80 90 100
STSRAKAEEQ GFTVKTPSDA SAWADVIVIL APDQHQRGLY ADSVRDNLTE
110 120 130 140 150
GKTLVFAHGF NIRFGYIEAP EGVDVVLVAP KGPGHTVRRE FEAGRGVPVI
160 170 180 190 200
VAVEVDASGK AWDLAWSYAK GIGGLRAGGI RTTFTEETET DLFGEQAVLC
210 220 230 240 250
GGTSQLVQYG FETLMEAGYQ PQIAYFEVLH ELKLIVDLMW EGGIAKQRWS
260 270 280 290 300
ISDTAEYGDY VSGPRVISPD VKENMKAVLA DIQSGAFADR FIKDQDAGAP
310 320 330 340
EFLELRKKGE EHPIESTGRE LRKLFAWNKA DDDYTDGSVA R
Length:341
Mass (Da):37,171
Last modified:June 12, 2007 - v1
Checksum:iAABD78C6E9F6740B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM711867 Genomic DNA Translation: CAN01139.1
RefSeqiWP_012037782.1, NC_009480.1

Genome annotation databases

EnsemblBacteriaiCAN01139; CAN01139; CMM_1096
KEGGicmi:CMM_1096

Similar proteinsi

Entry informationi

Entry nameiILVC_CLAM3
AccessioniPrimary (citable) accession number: A5CPY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: May 23, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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