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A5CD86

- DNAJ_ORITB

UniProt

A5CD86 - DNAJ_ORITB

Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Orientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

    Cofactori

    Binds 2 zinc ions per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi151 – 1511Zinc 1UniRule annotation
    Metal bindingi154 – 1541Zinc 1UniRule annotation
    Metal bindingi168 – 1681Zinc 2UniRule annotation
    Metal bindingi171 – 1711Zinc 2UniRule annotation
    Metal bindingi190 – 1901Zinc 2UniRule annotation
    Metal bindingi193 – 1931Zinc 2UniRule annotation
    Metal bindingi204 – 2041Zinc 1UniRule annotation
    Metal bindingi207 – 2071Zinc 1UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri138 – 21679CR-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. nucleic acid binding Source: InterPro
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. protein folding Source: InterPro
    3. response to heat Source: InterPro

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    DNA replication, Stress response

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciOTSU357244:GCA5-723-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chaperone protein DnaJUniRule annotation
    Gene namesi
    Name:dnaJUniRule annotation
    Ordered Locus Names:OTBS_0703
    OrganismiOrientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi)
    Taxonomic identifieri357244 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeOrientia
    ProteomesiUP000001565: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 377377Chaperone protein DnaJPRO_1000085237Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi357244.OTBS_0703.

    Structurei

    3D structure databases

    ProteinModelPortaliA5CD86.
    SMRiA5CD86. Positions 5-75.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 7066JUniRule annotationAdd
    BLAST
    Repeati151 – 1588CXXCXGXG motif
    Repeati168 – 1758CXXCXGXG motif
    Repeati190 – 1978CXXCXGXG motif
    Repeati204 – 2118CXXCXGXG motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi77 – 12145Gly-richAdd
    BLAST

    Domaini

    The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

    Sequence similaritiesi

    Belongs to the DnaJ family.UniRule annotation
    Contains 1 CR-type zinc finger.UniRule annotation
    Contains 1 J domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri138 – 21679CR-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0484.
    HOGENOMiHOG000226717.
    KOiK03686.
    OMAiKIITKKC.
    OrthoDBiEOG6BPDKP.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    HAMAPiMF_01152. DnaJ.
    InterProiIPR012724. DnaJ.
    IPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    IPR001878. Znf_CCHC.
    [Graphical view]
    PfamiPF01556. CTDII. 1 hit.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view]
    PRINTSiPR00625. JDOMAIN.
    SMARTiSM00271. DnaJ. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
    PROSITEiPS50076. DNAJ_2. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5CD86-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDLDYYQVL GVSRTASQEE IKRAYRKLVL KYHPDHNPGD KNAEQKIKNI    50
    NEAYDILKDE KKRSAYDQLG HQAFKNSGGG NYQQHHGFTG GIDPNDIFEN 100
    IFGDFMGARR SSKTAFSKKA GANLKYDISL TLEEAFYGVT KIISFKTALT 150
    CDACAGKGSL DNNSTSSCPT CRGSGVTRSQ QGFFFFENTC QTCRGAGHVI 200
    KNPCTKCYGE GRYINTRNLE VKIPAGVKEG SRIKLTGEGE AGSRGGKTGD 250
    LYVCITLIPH NTFSVDGNDL HCQLDINCTT AALGGEVEVA DITGSKLKLK 300
    IPAGTQNNHK LKLSGKGMQI LHSDRCGNMI VHVNIKVPKS LTKSQRELMI 350
    KLDKELNEAS EEGFLSKVRN FWASGSE 377
    Length:377
    Mass (Da):41,344
    Last modified:June 12, 2007 - v1
    Checksum:i044DE87692EC697D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM494475 Genomic DNA. Translation: CAM79769.1.
    RefSeqiWP_011944615.1. NC_009488.1.
    YP_001248495.1. NC_009488.1.

    Genome annotation databases

    EnsemblBacteriaiCAM79769; CAM79769; OTBS_0703.
    GeneIDi5219289.
    KEGGiots:OTBS_0703.
    PATRICi22822686. VBIOriTsu83812_0764.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM494475 Genomic DNA. Translation: CAM79769.1 .
    RefSeqi WP_011944615.1. NC_009488.1.
    YP_001248495.1. NC_009488.1.

    3D structure databases

    ProteinModelPortali A5CD86.
    SMRi A5CD86. Positions 5-75.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 357244.OTBS_0703.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAM79769 ; CAM79769 ; OTBS_0703 .
    GeneIDi 5219289.
    KEGGi ots:OTBS_0703.
    PATRICi 22822686. VBIOriTsu83812_0764.

    Phylogenomic databases

    eggNOGi COG0484.
    HOGENOMi HOG000226717.
    KOi K03686.
    OMAi KIITKKC.
    OrthoDBi EOG6BPDKP.

    Enzyme and pathway databases

    BioCyci OTSU357244:GCA5-723-MONOMER.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    HAMAPi MF_01152. DnaJ.
    InterProi IPR012724. DnaJ.
    IPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    IPR001878. Znf_CCHC.
    [Graphical view ]
    Pfami PF01556. CTDII. 1 hit.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view ]
    PRINTSi PR00625. JDOMAIN.
    SMARTi SM00271. DnaJ. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    TIGRFAMsi TIGR02349. DnaJ_bact. 1 hit.
    PROSITEi PS50076. DNAJ_2. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Orientia tsutsugamushi genome reveals massive proliferation of conjugative type IV secretion system and host-cell interaction genes."
      Cho N.-H., Kim H.-R., Lee J.-H., Kim S.-Y., Kim J., Cha S., Kim S.-Y., Darby A.C., Fuxelius H.-H., Yin J., Kim J.H., Kim J., Lee S.J., Koh Y.-S., Jang W.-J., Park K.-H., Andersson S.G.E., Choi M.-S., Kim I.-S.
      Proc. Natl. Acad. Sci. U.S.A. 104:7981-7986(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Boryong.

    Entry informationi

    Entry nameiDNAJ_ORITB
    AccessioniPrimary (citable) accession number: A5CD86
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3