Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A5CD86 (DNAJ_ORITB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chaperone protein DnaJ
Gene names
Name:dnaJ
Ordered Locus Names:OTBS_0703
OrganismOrientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi) [Complete proteome] [HAMAP]
Taxonomic identifier357244 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeOrientia

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins By similarity. HAMAP-Rule MF_01152

Cofactor

Binds 2 zinc ions per monomer By similarity. HAMAP-Rule MF_01152

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01152

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01152.

Domain

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity By similarity. HAMAP-Rule MF_01152

Sequence similarities

Belongs to the DnaJ family.

Contains 1 CR-type zinc finger.

Contains 1 J domain.

Ontologies

Keywords
   Biological processDNA replication
Stress response
   Cellular componentCytoplasm
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionChaperone
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

protein folding

Inferred from electronic annotation. Source: InterPro

response to heat

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: InterPro

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 377377Chaperone protein DnaJ HAMAP-Rule MF_01152
PRO_1000085237

Regions

Domain5 – 7066J
Repeat151 – 1588CXXCXGXG motif HAMAP-Rule MF_01152
Repeat168 – 1758CXXCXGXG motif HAMAP-Rule MF_01152
Repeat190 – 1978CXXCXGXG motif HAMAP-Rule MF_01152
Repeat204 – 2118CXXCXGXG motif HAMAP-Rule MF_01152
Zinc finger138 – 21679CR-type HAMAP-Rule MF_01152
Compositional bias77 – 12145Gly-rich HAMAP-Rule MF_01152

Sites

Metal binding1511Zinc 1 By similarity
Metal binding1541Zinc 1 By similarity
Metal binding1681Zinc 2 By similarity
Metal binding1711Zinc 2 By similarity
Metal binding1901Zinc 2 By similarity
Metal binding1931Zinc 2 By similarity
Metal binding2041Zinc 1 By similarity
Metal binding2071Zinc 1 By similarity

Sequences

Sequence LengthMass (Da)Tools
A5CD86 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 044DE87692EC697D

FASTA37741,344
        10         20         30         40         50         60 
MSDLDYYQVL GVSRTASQEE IKRAYRKLVL KYHPDHNPGD KNAEQKIKNI NEAYDILKDE 

        70         80         90        100        110        120 
KKRSAYDQLG HQAFKNSGGG NYQQHHGFTG GIDPNDIFEN IFGDFMGARR SSKTAFSKKA 

       130        140        150        160        170        180 
GANLKYDISL TLEEAFYGVT KIISFKTALT CDACAGKGSL DNNSTSSCPT CRGSGVTRSQ 

       190        200        210        220        230        240 
QGFFFFENTC QTCRGAGHVI KNPCTKCYGE GRYINTRNLE VKIPAGVKEG SRIKLTGEGE 

       250        260        270        280        290        300 
AGSRGGKTGD LYVCITLIPH NTFSVDGNDL HCQLDINCTT AALGGEVEVA DITGSKLKLK 

       310        320        330        340        350        360 
IPAGTQNNHK LKLSGKGMQI LHSDRCGNMI VHVNIKVPKS LTKSQRELMI KLDKELNEAS 

       370 
EEGFLSKVRN FWASGSE 

« Hide

References

[1]"The Orientia tsutsugamushi genome reveals massive proliferation of conjugative type IV secretion system and host-cell interaction genes."
Cho N.-H., Kim H.-R., Lee J.-H., Kim S.-Y., Kim J., Cha S., Kim S.-Y., Darby A.C., Fuxelius H.-H., Yin J., Kim J.H., Kim J., Lee S.J., Koh Y.-S., Jang W.-J., Park K.-H., Andersson S.G.E., Choi M.-S., Kim I.-S.
Proc. Natl. Acad. Sci. U.S.A. 104:7981-7986(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Boryong.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM494475 Genomic DNA. Translation: CAM79769.1.
RefSeqYP_001248495.1. NC_009488.1.

3D structure databases

ProteinModelPortalA5CD86.
SMRA5CD86. Positions 5-75.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING357244.OTBS_0703.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAM79769; CAM79769; OTBS_0703.
GeneID5219289.
KEGGots:OTBS_0703.
PATRIC22822686. VBIOriTsu83812_0764.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0484.
HOGENOMHOG000226717.
KOK03686.
OMAENKGAFG.
OrthoDBEOG6BPDKP.
ProtClustDBCLSK2325730.

Enzyme and pathway databases

BioCycOTSU357244:GCA5-723-MONOMER.

Family and domain databases

Gene3D1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPMF_01152. DnaJ.
InterProIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR001878. Znf_CCHC.
[Graphical view]
PfamPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSPR00625. JDOMAIN.
SMARTSM00271. DnaJ. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsTIGR02349. DnaJ_bact. 1 hit.
PROSITEPS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNAJ_ORITB
AccessionPrimary (citable) accession number: A5CD86
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families