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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Orientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi151Zinc 1UniRule annotation1
Metal bindingi154Zinc 1UniRule annotation1
Metal bindingi168Zinc 2UniRule annotation1
Metal bindingi171Zinc 2UniRule annotation1
Metal bindingi190Zinc 2UniRule annotation1
Metal bindingi193Zinc 2UniRule annotation1
Metal bindingi204Zinc 1UniRule annotation1
Metal bindingi207Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri138 – 216CR-typeUniRule annotationAdd BLAST79

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processDNA replication, Stress response
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciOTSU357244:G1GJM-735-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:OTBS_0703
OrganismiOrientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi)
Taxonomic identifieri357244 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeOrientia
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000852371 – 377Chaperone protein DnaJAdd BLAST377

Proteomic databases

PRIDEiA5CD86

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi357244.OTBS_0703

Structurei

3D structure databases

ProteinModelPortaliA5CD86
SMRiA5CD86
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 70JUniRule annotationAdd BLAST66
Repeati151 – 158CXXCXGXG motif8
Repeati168 – 175CXXCXGXG motif8
Repeati190 – 197CXXCXGXG motif8
Repeati204 – 211CXXCXGXG motif8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi77 – 121Gly-richAdd BLAST45

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri138 – 216CR-typeUniRule annotationAdd BLAST79

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG4105BZ5 Bacteria
COG0484 LUCA
HOGENOMiHOG000226717
KOiK03686
OMAiDMGGFAD
OrthoDBiPOG091H00PT

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
HAMAPiMF_01152 DnaJ, 1 hit
InterProiView protein in InterPro
IPR012724 DnaJ
IPR002939 DnaJ_C
IPR001623 DnaJ_domain
IPR008971 HSP40/DnaJ_pept-bd
IPR001305 HSP_DnaJ_Cys-rich_dom
IPR036410 HSP_DnaJ_Cys-rich_dom_sf
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF01556 DnaJ_C, 1 hit
PF00684 DnaJ_CXXCXGXG, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF49493 SSF49493, 3 hits
SSF57938 SSF57938, 1 hit
TIGRFAMsiTIGR02349 DnaJ_bact, 1 hit
PROSITEiView protein in PROSITE
PS50076 DNAJ_2, 1 hit
PS51188 ZF_CR, 1 hit

Sequencei

Sequence statusi: Complete.

A5CD86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLDYYQVL GVSRTASQEE IKRAYRKLVL KYHPDHNPGD KNAEQKIKNI
60 70 80 90 100
NEAYDILKDE KKRSAYDQLG HQAFKNSGGG NYQQHHGFTG GIDPNDIFEN
110 120 130 140 150
IFGDFMGARR SSKTAFSKKA GANLKYDISL TLEEAFYGVT KIISFKTALT
160 170 180 190 200
CDACAGKGSL DNNSTSSCPT CRGSGVTRSQ QGFFFFENTC QTCRGAGHVI
210 220 230 240 250
KNPCTKCYGE GRYINTRNLE VKIPAGVKEG SRIKLTGEGE AGSRGGKTGD
260 270 280 290 300
LYVCITLIPH NTFSVDGNDL HCQLDINCTT AALGGEVEVA DITGSKLKLK
310 320 330 340 350
IPAGTQNNHK LKLSGKGMQI LHSDRCGNMI VHVNIKVPKS LTKSQRELMI
360 370
KLDKELNEAS EEGFLSKVRN FWASGSE
Length:377
Mass (Da):41,344
Last modified:June 12, 2007 - v1
Checksum:i044DE87692EC697D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM494475 Genomic DNA Translation: CAM79769.1
RefSeqiWP_011944615.1, NC_009488.1

Genome annotation databases

EnsemblBacteriaiCAM79769; CAM79769; OTBS_0703
GeneIDi29642156
KEGGiots:OTBS_0703

Similar proteinsi

Entry informationi

Entry nameiDNAJ_ORITB
AccessioniPrimary (citable) accession number: A5CD86
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: May 23, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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