Reviewed, UniProtKB/Swiss-Prot A5CD02 (SYE1_ORITB)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	OTBS_0573

Organism

Orientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi) [Complete proteome] [HAMAP]

Taxonomic identifier

357244 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Orientia

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm By similarity HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000367725
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022
	Motif	242 – 246	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	245	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5CD02-1 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: 142483D2A096C22B	FASTA	478	53,883
	10 20 30 40 50 60 MTNIVTRFAP SPTGFLHIGG ARTALFNYLF ARHHKGKFLL RIEDTDAARS TEEYKISIID 70 80 90 100 110 120 SLKWLKINWD NDIFYQSANL QRHVNIALEL VKSGKAYYCF TSPEEIDLQR QLAITQKQSF 130 140 150 160 170 180 IFRSPWRNNI PSSLSLKNNN KAYVIRFKAP DYGTTIINDH VQGEVVFQNQ QIDDMILVRS 190 200 210 220 230 240 DGTPTYMLAV VVDDHDMGIT HIIRGDDHLT NAAKQIALYD ALGWAAPAMV HIPLIYGPDG 250 260 270 280 290 300 TKLSKRHGAI GVDAYQKMGY LPEALCNYLL RLGWSYQDEE IISHERAIKL FDIDGLGGKS 310 320 330 340 350 360 AARLDFDKML YLNGYYIRST DNSILAKLVI EILSKQYILS NESCNLIQKG MSGLKSRANL 370 380 390 400 410 420 LTDLAENAKI YVLESQLTFI NEALNIIQKT PSMLITEVID IINNLQELNC ESVKQALTEF 430 440 450 460 470 AKTKKMKLGQ LMDPIRALLT GNTKSPSIFE VIPILGKIHT IKRLAGIKAI KSNNQTLV

References

[1]

"The Orientia tsutsugamushi genome reveals massive proliferation of conjugative type IV secretion system and host-cell interaction genes." Cho N.-H., Kim H.-R., Lee J.-H., Kim S.-Y., Kim J., Cha S., Kim S.-Y., Darby A.C., Fuxelius H.-H., Yin J., Kim J.H., Kim J., Lee S.J., Koh Y.-S., Jang W.-J., Park K.-H., Andersson S.G.E., Choi M.-S., Kim I.-S. Proc. Natl. Acad. Sci. U.S.A. 104:7981-7986(2007) [PubMed: 17483455] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM494475 Genomic DNA. Translation: CAM79639.1. Different initiation.
RefSeq	YP_001248424.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5CD02.
Genome annotation databases
GeneID	5218910.
GenomeReviews	Gene locus OTBS_0573 in contig AM494475_GR.
KEGG	ots:OTBS_0573.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_ORITB

Accession

Primary (citable) accession number: A5CD02

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	January 19, 2010
This is version 21 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)